Summary
A spring wheat genotype which produces somatic embryos in vitro, after short and long-term culture, was tested for its ability to sexually transmit this embryogenic trait. Reciprocal crosses were performed between a embryogenic line and a nonembryogenic variety.
Immature embryos were cultured on Murashige and Skoog medium plus 2 mg/l 2,4-dichlorophenoxyacetic acid, gelled with 5.5 g/l agarose. Somatic embryogenesis was not expressed in the F1's. In contrast, from several hundred immature embryos of the F2 generation of one cross, 10.7% and 1.6% expressed somatic embryogenesis in short and long-term cultures respectively. These percentages of embryogenic: non-embryogenic fits a model of a few complementary genes. The embryogenic capacity of the F2 genotypes depends on the presence of recessive alleles at these gene loci. The long-term wheat somatic embryogenesis capacity requires a more complex mechanism than the short-term one.
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Abbreviations
- CS:
-
Chinese Spring
- Aq:
-
Aquila
- E:
-
Embryogenic
- NE:
-
Nonembryogenic
- SC:
-
Subculture
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De Buyser, J., Marcotte, J.L. & Henry, Y. Genetic analysis of in vitro wheat somatic embryogenesis. Euphytica 63, 265–270 (1992). https://doi.org/10.1007/BF00024553
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DOI: https://doi.org/10.1007/BF00024553