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The current status of physiology and biochemistry of brassinosteroids

A review

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Abstract

Brassinolide, first isolated from pollen of rape as a plant growth promoting substance, has been found to be widely distributed in the plant kingdom. Over thirty endogenous analogues, called collectively brassinosteroids, have been isolated and identified. As a new class of plant hormones, brassinosteroids show not only growth promoting activity but also other physiological effects on the growth and development of plants and draw attention as promising chemicals for practical application in agriculture. This review describes the current status of the studies on the natural occurrence, analysis, physiological actions, metabolism and biosynthesis of brassinosteroids.

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Abbreviations

ABA:

abscisis acid

BR:

brassinosteroid

GA:

gibberellin

GC-MS:

combined gas chromatography-mass spectrometry

GC-SIM:

combined gas chromatography-selected ion monitoring

HPLC:

high performance liquid chromatography

IAA:

indole-3-acetic acid

References

  1. Abe H (1991) Rice-lamina inclination, endogenous levels in plant tissues and accumulation during pollen development of brassinosteroids. In: Cutler HG, Yokota T and Adam G, eds, Brassinosteroids, pp 200–207. American Chemical Society, Washington, DC

    Google Scholar 

  2. Abe H, Morishita T, Uchiyama M, Marumo S, Munakata K, Takatsuto S and Ikekawa N (1982) Identification of brassinolide-like substances in Chinese cabbage. Agric Biol Chem 46: 2609–2611

    Google Scholar 

  3. Abe H, Morishita T, Uchiyama M, Takatsuto S and Ikekawa N (1984) A new brassinolide-related steroid in the leaves of Thea sinensis. Agric Biol Chem 48: 2171–2172

    Google Scholar 

  4. Abe H, Morishita T, Uchiyama M, Takatsuto S, Ikekawa N, Ikeda M, Sassa T, Kitsuwa T and Marumo S (1983) Occurrence of three new brassinosteroids: brassinone, (24S)-24-ethylbrassinone and 28-norbrassinolide, in higher plants. Experientia 39: 351–353

    Google Scholar 

  5. Abe H, Nakamura K, Morishita T, Uchiyama M, Takatsuto S and Ikekawa N (1984) Endogenous brassinosteroids of the rice plant: Castasterone and dolichosterone. Agric Biol Chem 48: 1103–1104

    Google Scholar 

  6. Arima M, Yokota T and Takahashi N (1984) Identification and quantification of brassinolide-related steroids in the insect gall and healthy tissues of the chestnut plant. Phytochemistry 23: 1587–1591

    Article  Google Scholar 

  7. Arteca RN and Bachman JM (1987) Light inhibition of brassinosteroid-induced ethylene production. J Plant Physiol 129: 13–18

    Google Scholar 

  8. Arteca RN, Bachman JM and Mandava NB (1988) Effects of indole-3-acetic acid and brassinosteroid on ethylene biosynthesis in etiolated mung bean hypocotyl segments. J Plant Physiol 133: 430–435

    Google Scholar 

  9. Arteca RN, Bachman JM, Tsai D-S and Mandava NB (1988) Fusicoccin, an inhibitor of brassinosteroid-induced ethylene production. Physiol Plant 74: 631–634

    Google Scholar 

  10. Baba J, Yokota T and Takahashi N (1983) Brassinoliderelated new bioactive steroids from Dolichos lablab seed. Agric Biol Chem 47: 659–661

    Google Scholar 

  11. Bellincampi D and Morpurgo G (1988) Stimulation of growth in Daucus carota L. cell cultures by brassinosteroid. Plant Science 54: 153–156

    Article  Google Scholar 

  12. Cerana R, Bonetti A, Marre MT, Romani G, Lado P and Marre E (1983) Effects of a brassinosteroid on growth and electrogenic proton extrusion in Azuki bean epicotyls. Physiol Plant 59: 23–27

    Google Scholar 

  13. Clouse SD and Zurek D (1991) Molecular analysis of brassinolide action in plant growth and development. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids. pp 122–140, American Chemical Society, Washington, DC

    Google Scholar 

  14. Cohen JD and Meudt WJ (1983) Investigations on the mechanism of the brassinosteroid response. I. Indole-3-acetic acid metabolism and transport. Plant Physiol 72: 691–694

    Google Scholar 

  15. Cutler HG, Yokota T and Adam G, eds. (1991) Brassinosteroids. American Chemical Society, Washington, DC (Each article in the book is cited occasionally as the reference.)

    Google Scholar 

  16. Eun J-S, Kuraishi S Sakurai N (1989) Changes in levels of auxin and abscisic acid and the evolution of ethylene in squash hypocotyls after treatment with brassinolide. Plant Cell Physiol 30: 807–810

    Google Scholar 

  17. Gamoh K, Okamoto N, Takatsuto S and Tejima I (1990) Determination of traces of natural brassinosteroids as dansylaminophenylboronates by liquid chromatography with fluorimetric detection. Anal Chim Acta 228: 101–105

    Article  Google Scholar 

  18. Gamoh K, Omote K, Okamoto N and Takatsuto S (1989) High-performance liquid chromatography of brassinosteroids in plants with derivatization using 9-phenanthreneboronic acid. J Chromatogr 469: 424–428

    Article  Google Scholar 

  19. Gamoh K, Sawamoto H, Takatsuto S, Watabe Y and Arimoto H (1990) Ferroceneboronic acid as a derivatization reagent for the determination of brassinosteroids by high-performance liquid chromatography with electrochemical detection. J Chromatogr 515: 227–231

    Article  Google Scholar 

  20. Gamoh K and Takatsuto S (1989) A boronic acid derivative as a highly sensitive fluorescence derivatization reagent for brassinosteroids in liquid chromatography. Anal Chim Acta 222: 201–204

    Article  Google Scholar 

  21. Gartz J, Adam G and Vorbrodt H-M (1990) Growth-promoting effect of a brassinosteroid in mycelial cultures of the fungus Psilocybe cubensis. Naturwissenschaften 77: 388–389

    Google Scholar 

  22. Grove MD, Spencer GF, Rohwedder WK, Mandava NB, Worley JF, WarthenJr JD, Steffens GL, Flippen-Anderson JL and CookJr JC (1979) Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature 281: 216–217.

    Google Scholar 

  23. He R-Y, Wang G-J and Wang X-S (1991) Effects of brassinolide on growth and chilling resistance of maize seedlings. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 220–230, American Chemical Society, Washington, DC

    Google Scholar 

  24. Ikeda M, Takatsuto S, Sassa T, Ikekawa N and Nukina M (1983) Identification of brassinolide and its analogues in chestnut gall tissue. Agric Biol Chem 47: 655–657

    Google Scholar 

  25. Ikekawa N, Nishiyama F and Fujimoto Y (1988) Identification of 24-epibrassinolide in bee pollen of the broad bean, Vicia faba L. Chem Pharm Bull 36: 405–407

    Google Scholar 

  26. Ikekawa N, Takatsuto S, Kitsuwa T, Saito H, Morishita T and Abe H (1984) Analysis of natural brassinosteroids by gas chromatography and gas chromatography-mass spectrometry. J Chromatogr 290: 289–302

    Article  Google Scholar 

  27. Iwahori S, Tominaga S and Higuchi S (1990) Retardation of abscission of citrus leaf and fruitlet explants by brassinolide. Plant Growth Regul 9: 119–125

    Google Scholar 

  28. Iwasaki T and Shibaoka H (1991) Brassinosteroids act as regulators of tracheary-element differentiation in isolated Zinnia mesophyll cells. Plant Cell Physiol 32: 1007–1014

    Google Scholar 

  29. Kalinich JF, Mandava NB and Todhunter JA (1986) Relationship of nucleic acid metabolism to brassinolide-induced responses in beans. J Plant Physiol 125: 345–353

    Google Scholar 

  30. Kamuro Y and Inada K (1991) The effect of brassinolide on the light-induced growth inhibition in mung bean epicotyl. Plant Growth Regul 10: 37–43

    Google Scholar 

  31. Katsumi M (1985) Interaction of a brassinosteroid with IAA and GA3 in the elongation of cucumber hypocotyl sections. Plant Cell Physiol 26: 615–625

    Google Scholar 

  32. Katsumi M (1991) Physiological modes of brassinolide action in cucumber hypocotyl growth. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 246–254, American Chemical Society, Washington, DC

    Google Scholar 

  33. Kim S-K (1991) Natural occurrences of brassinosteroids. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 26–35. American Chemical Society, Washington, DC

    Google Scholar 

  34. Kim S-K, Abe H, Little CHA and Pharis RP (1990) Identification of two brassinosteroids from the cambial region Scots pine (Pinus silverstris) by gas chromatography-mass spectrometry, after detection using a dwarf rice lamina inclination bioassay. Plant Physiol 94: 1709–1713

    Google Scholar 

  35. Kim S-K, Akihisa T, Tamura T, Matsumoto T, Yokota T and Takahashi N. (1988) 24-Methylene-25-methylchole-sterol in Phaseolus vulgaris seed: Structural relation to brassinosteroids. Phytochemistry 27: 629–631

    Article  Google Scholar 

  36. Kim S-K, Yokota T and Takahashi N (1987) 25-Methyldolichosterone, a new brassinosteroid with a tertiary butyl group from immature seed of Phaseolus vulgaris. Agric Biol Chem 51: 2303–2305

    Google Scholar 

  37. Kulaeva ON, Burkhanova EA, Fedina AB, Khokhlova VA, Bokebayeva GA, Vorbrodt HM and Adam G (1991) Effect of brassinosteroids on protein synthesis and plant-cell ultrastructure under stress conditions. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 141–155, American Chemical Society, Washington, DC

    Google Scholar 

  38. Lehmann M, Vorbrodt H-M, Adam G and Koolman J (1988) Antiecdysteroid activity of brassinosteroids. Experientia 44: 355–356

    Google Scholar 

  39. Mandava NB (1988) Plant growth-promoting brassinosteroids. Ann Rev Plant Physiol Plant Mol Biol 39: 23–52

    Article  Google Scholar 

  40. Meudt WJ (1987) Investigations on the mechanism of the brassinosteroid response. VI. Effect of brassinolide on gravitropism of bean hypocotyls. Plant Physiol 83: 195–198

    Google Scholar 

  41. Morishita T, Abe H, Uchiyama M, Murumo S, Takatsuto S and Ikekawa N (1983) Evidence for plant growth promoting brassinosteroids in leaves of Thea sinensis. Phytochemistry 22: 1051–1053

    Article  Google Scholar 

  42. Park K-H, Saimoto H, Nakagawa S, Sakurai A, Yokota T, Takahashi N and Syõno K (1989) Occurrence of brassinolide and castasterone in crown gall cells of Catharanthus roseus. Agric Biol Chem 53: 805–811

    Google Scholar 

  43. Park K-H, Yokota T, Sakurai A and Takahashi N (1987) Occurrence of castasterone, brassinolide and methyl 4-chloroindole-3-acetate in immature Vicia faba seeds. Agric Biol Chem 51: 3081–3086

    Google Scholar 

  44. Plattner RD, Taylor SL and Grove MD (1986) Detection of brassinolide and castasterone in Alnus glutinosa (European alder) pollen by mass spectrometry/mass spectrometry. J Nat Prod 49: 540–545

    Google Scholar 

  45. Roddick JG and Guan M (1991) Brassinosteroids and root development. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 231–245, American Chemical Society, Washington, DC

    Google Scholar 

  46. Roddick JG and Ikekawa N (1992) Modification of root and shoot development in monocotyledon and dicotyledon seedlings by 24-epibrassinolide. J Plant Physiol 140: 70–74

    Google Scholar 

  47. Roth PS, Bach TJ and Thompson MJ (1989) Brassinosteroids: Potent inhibitors of growth of transformed tobacco callus cultures. Plant Science 59: 63–70

    Article  Google Scholar 

  48. Sakurai A, Fujioka S and Saimoto H (1991) Production of brassinosteroids in plant-cell cultures. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 97–106, American Chemical Society, Washington, DC

    Google Scholar 

  49. Sala C and Sala F (1985) Effect of brassinosteroid on cell division and enlargement in cultured carrot (Daucus carota L.) cells. Plant Cell Reports 4: 144–147

    Google Scholar 

  50. Sasse JM (1990) Brassinolide-induced elongation and auxin. Physiol Plant 80: 401–408

    Article  Google Scholar 

  51. Sasse JM (1991) The case for brassinosteroids as endogenous plant hormones. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 158–166, American Chemical Society, Washington, DC

    Google Scholar 

  52. Sasse JM, Yokota T, Taylor PE, Griffiths PG, Porter QN and Cameron DW (1992) Brassinolide-induced elongation. In: Karssen CM, van Loon LC and Vreugdenhil D eds. Progress in plant growth regulation. pp 319–325, Dordrecht, Boston, London, Kluwer Academic Publishers

    Google Scholar 

  53. Sathiyamoorthy P and Nakamura S (1990) In vitro root induction by 24-epibrassinolide on hypocotyl segments of soybean [Glycine max (L.) Merr.]. Plant Growth Regul 9: 73–76

    Google Scholar 

  54. Schilling G, Schiller C and Otto S (1991) Influence of brassinosteroids on organ relations and enzyme activities of sugar-beet plants. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 208–219, American Chemical Society, Washington, DC

    Google Scholar 

  55. Schlagnhaufer CD and Arteca RD (1991) The uptake and metabolism of brassinosteroid by tomato (Lycopersicon esculentum) plants. J Plant Physiol 138: 191–194

    Google Scholar 

  56. Schmidt J, Yokota T, Adam G and Takahashi N (1991) Castasterone and brassinolide in Raphanus sativus seeds. Phytochemistry 30: 364–365

    Article  Google Scholar 

  57. Schneider JA, Yoshihara K, Nakanishi K and Kato N (1983) Typhasterol (2-deoxycastasterone): A new palnt growth regulator from cat-tail pollen. Tetrahedron Letters 24: 3859–3860

    Article  Google Scholar 

  58. Suzuki Y, Yamaguchi I and Takahashi N (1985) Identification of castasterone and brassinone from immature seeds of Pharbitis purpurea. Agric Biol Chem 49: 49–54

    Google Scholar 

  59. Suzuki Y, Yamaguchi I, Yokota T and Takahashi N (1986) Identification of castasterone, typhasterol and teasterone from the pollen of Zea mays. Agric Biol Chem 50: 3133–3138

    Google Scholar 

  60. Takatsuto S (1991) Microanalysis of naturally occurring braassinosteroids. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids, pp 107–120. American Chemical Society, Washington, DC

    Google Scholar 

  61. Takatsuto S, Abe H and Gamoh K (1990) Evidence for brassinosteroids in strobilus of Equisetum arvense L. Agric Biol Chem 54: 1057–1059

    Google Scholar 

  62. Takatsuto S and Ikekawa N (1986) Synthesis of [26,28-2H6]brassinolide, [26,28-2H6]castasterone, [26,28-2H6]typhasterol, and [26,28-2H6]teasterone. Chem Pharm Bull 34: 1415–1418

    Google Scholar 

  63. Takatsuto S, Omote K, Gamoh K and Ishibashi M (1990) Identification of brassinolide and castasterone in buckwheat (Fagopyrum esculentum Moench) pollen. Agric Biol Chem 54: 757–762

    Google Scholar 

  64. Takatsuto S, Ying B, Morisaki M and Ikekawa N (1982) Microanalysis of brassinolide and its analogues by gas chromatography and gas chromatography-mass spectrometry. J Chromatogr 239: 233–241

    Article  Google Scholar 

  65. Takatsuto S, Yokota T, Omote K, Gamoh K and Takahashi N (1989) Identification of brassinolide, castasterone and norcastasterone (brassinone) in sunflower (Helianthus annuus L.) pollen. Agric Biol Chem 53: 2177–2180

    Google Scholar 

  66. Takematsu T, Takeuchi Y and Koguchi M (1985) New plant growth regulators. Brassinolide analogues: Their biological effects and application to agriculture and biomass production. Shokucho 18: 2–15 (in Japanese)

    Google Scholar 

  67. Takeno K and Pharis RP (1982) Brassinosteroid-induced bending of the leaf lamina of dwarf rice seedlings: An auxin-mediated phenomenon. Plant Cell Physiol 23: 1275–1281

    Google Scholar 

  68. Wada K, Kondo H and Marumo S (1985) A simple bioassay for brassinosteroids: A wheat leaf-unrolling test. Agric Biol Chem 49: 2249–2251

    Google Scholar 

  69. Wada K, Marumo S, Ikekawa N, Morisaki M and Mori K (1981) Brassinolide and homobrassinolide promotion of lamina inclination of rice seedlings. Plant Cell Physiol 22: 323–325

    Google Scholar 

  70. Yokota T, Arima M and Takahashi N (1982) Castasterone, a new phytosterol with plant-hormone potency, from chestnut insect gall. Tetrahedron Letters 23: 1275–1278

    Article  Google Scholar 

  71. Yokota T, Arima M, Takahashi N and Crozier A (1985) Steroidal plant growth regulators, castasterone and typhasterol (2-deoxycastasterone) from the shoots of Sitka spruce (Pices sitchensis). Phytochemistry 24: 1333–1335

    Article  Google Scholar 

  72. Yokota T, Arima M, Takahashi N, Takatsuto S, Ikekawa N and Takematsu T (1983) 2-Deoxycastasterone, a new brassinolide-related bioactive steroid from Pinus pollen. Agric Biol Chem 47: 2419–2420

    Google Scholar 

  73. Yokota T, Baba J, Koba S and Takahashi N (1984) Purification and separation of eight steroidal plant-growth regulators from Dolichos lablab seed. Agric Biol Chem 48: 2529–2534

    Google Scholar 

  74. Yokota T, Baba J and Takahashi N (1982) A new steroidal lactone with plant growth-regulatory activity from Dolichos lablab seed. Tetrahedron Letters 23: 4965–4966

    Article  Google Scholar 

  75. Yokota T, Baba J and Takahashi N (1983) Brassinoliderelated bioactive sterols in Dolichos lablab: Brassinolide, castasterone and a new analog, homodolicholide. Agric Biol Chem 47: 1409–1411

    Google Scholar 

  76. Yokota T, Higuchi K, Kosaka Y and Takahashi N (1992) Transport and metabolism of brassinosteroids in rice. In: Karssen CM, van Loon LC and Vreugdenhil D eds. Progress in plant growth regulation. pp 298–305, Dordrecht, Boston, London, Kluwer Academic Publishers

    Google Scholar 

  77. Yokota T, Kim S-K, Fukui Y, Takahashi N, Takeuchi Y and Takematsu T (1987) Brassinosteroids and sterols from a green alga, Hydrodictyon reticulatum: Configuration at C-24. Phytochemistry 26: 503–506

    Article  Google Scholar 

  78. Yokota T, Kim S-K, Kosaka Y, Ogino Y and Takahashi N (1987) Conjugation of brassinosteroids. In: Schreiber K, Schutte HE, Sembdner G eds. Conjugated plant hormone, structure, metabolism and function, pp 288-296. VER Deutscher Verlag der Wissenschaften

  79. Yokota T, Koba S, Kim S-K, Takatsuto S, Ikekawa N, Sakakibara M, Okada K, Mori K and Takahashi N (1987) Diverse structural variations of the brassinosteroids in Phaseolus vulgaris seed. Agric Biol Chem 51: 1625–1631

    Google Scholar 

  80. Yokota T, Morita M and Takahashi N (1983) 6-Deoxocastasterone and 6-deoxodolichosterone: Putative precursors for brassinolide-related steroids from Phaseolus vulgaris. Agric Biol Chem 47: 2149–2151

    Google Scholar 

  81. Yokota T, Nakamura N, Takahashi N, Nonaka M, Sekimoto H, Oshio H and Takatsuto S (1990) Inconsistency between growth and endogenous levels of gibberellins, brassinosteroids, and sterols in Pisum sativum treated with uniconazole antipodes. In: Takahashi N, Phinney BO and MacMillan J eds. Gibberellins. pp 339–349, Springer, New York

    Google Scholar 

  82. Yokota T, Ogino Y, Suzuki H, Takahashi N, Saimoto H, Fujioka S and Sakurai A (1991) Metabolism and biosynthesis of brassinosteroids. In: Cutler HG, Yokota T and Adam G eds. Brassinosteroids. pp 86–96, American Chemical Society, Washington, DC

    Google Scholar 

  83. Yokota T, Watanabe S, Ogino Y, Yamaguchi I and Takahashi N (1990) Radioimmunoassay for brassinosteroids and its use for comparative analysis of brassinosteroids in stems and seeds of Phaseolus vulgaris. J Plant Growth Regul 9: 151–159

    Google Scholar 

  84. Yokota T, Ogino Y, Takahashi N, Saimoto H, Fujioka S and Sakurai A (1990) Brassinolide is biosynthesized from castasterone in Catharanthus roseus crown gall cells. Agric Biol Chem 54: 1107–1108

    Google Scholar 

  85. Yopp JH, Mandava NB and Sasse JM (1981) Brassinolide, a growth-promoting steroidal lactone. I. Activity in selected auxin bioassays. Physiol Plant 53: 445–452

    Google Scholar 

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Sakurai, A., Fujioka, S. The current status of physiology and biochemistry of brassinosteroids. Plant Growth Regul 13, 147–159 (1993). https://doi.org/10.1007/BF00024257

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