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Characterization and in situ localization of a salt-induced tomato peroxidase mRNA

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Abstract

NaCl treatment of tomato plants in hydroponic culture at concentrations as low as 50 mM resulted in enhanced accumulation of transcripts of TPX1, a full-length cDNA clone that we had isolated from a library of NaCl-treated tomato plants using a peroxidase-specific oligonucleotide probe. Although the overall amino acid sequence identity of TPX1 to other peroxidase genes was less than 45%, there was a very high degree of identity in all of the conserved domains. The deduced amino acid sequence included the presence of a N-terminal signal peptide but not the C-terminal extension present in peroxidases targeted to the vacuole. The mature protein has a theoretical pI value of 7.5. Transcripts that hybridized to TPX1 were detected only in the roots with higher levels of mRNA in epidermal and subepidermal cell layers. Isoelectric focusing of root extracts showed two major bands of peroxidase activity at pI 5.9 and 6.2. Both activities increased with salt treatment. Southern analysis indicated the presence of only a single TPX1 gene in tomato.

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular, Universidad de Málaga, 29071, Málaga, Spain

    Miguel A. Botella & Victoriano Valpuesta

  2. Departamento de Biología Vegetal, Universidad de Málaga, 29071, Málaga, Spain

    Miguel A. Quesada & Fernando Pliego

  3. Center for Plant Environmental Stress Physiology, Purdue University, 1165 Horticulture Building, 47907-1165, West Lafayette, In, USA

    Andrzej K. Kononowicz, Ray A. Bressan & Paul M. Hasegawa

  4. Department of Plant Cytology & Cytochemistry, University of Lodz, 90-237, Lodz, Poland

    Andrzej K. Kononowicz

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  1. Miguel A. Botella
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  2. Miguel A. Quesada
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  3. Andrzej K. Kononowicz
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  4. Ray A. Bressan
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  5. Fernando Pliego
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  6. Paul M. Hasegawa
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  7. Victoriano Valpuesta
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Botella, M.A., Quesada, M.A., Kononowicz, A.K. et al. Characterization and in situ localization of a salt-induced tomato peroxidase mRNA. Plant Mol Biol 25, 105–114 (1994). https://doi.org/10.1007/BF00024202

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  • DOI: https://doi.org/10.1007/BF00024202

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