Abstract
A highly transcribed region in Oenothera mitochondria codes for a reading frame (orf206) which shows high homology to the Marchantia encoded mitochondrial open reading frame orf277 and is also conserved in the mitochondrial genomes of Arabidopsis thaliana and Daucus carota. Transcripts of orf206 are modified by cytidine to uridine changes in 46 positions by RNA editing, affecting 30% of all cytidines and 15% of the total encoded amino acids. This ORF is contranscribed with an upstream reading frame and with the downstream rps14 gene. The orf206 deduced protein shows high similarity to polypeptides which are proposed to be part of an ABC-type heme transporter involved in cytochrome c biogenesis in Bradyrhizobium and Rhodobacter.
Similar content being viewed by others
References
Ames GF-L: Bacterial periplasmic transport systems: structure, mechanism, and evolution. Annu Rev Biochem 55: 397–425 (1986).
Beckman DL, Kranz RG: Cytochrome c biogenesis in a photosynthetic bacterium requires a periplasmic thioredoxin-like protein. Proc Natl Acad Sci USA 90: 2179–2183 (1993).
Beckman DL, Trawick DR, Kranz RG: Bacterial cytochrome c biogenesis. Genes Devel 6: 268–283 (1992).
Bibb MJ, VanEtten RA, Wright CT, Walberg MW, Clayton DA: Sequence and gene organization of mouse mitochondrial DNA. Cell 26: 167–180 (1981).
Covello PS, Gray MW: RNA editing in plant mitochondria. Nature 341: 662–666 (1989).
Gonzalez DH, Bonnard G, Grienenberger J-M: A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria. Curr Genet 24: 248–255 (1993).
Gray MW: The evolutionary origins of organelles. Trends Genet 5: 294–299 (1989).
Gray MW: The endosymbiont hypothesis revisited. Int Rev Cytol 141: 233–357 (1992).
Gualberto JM, Lamattina L, Bonnard G, Weil J-H, Grienenberger J-M: RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature 341: 660–662 (1989).
Hiesel R, Wissinger B, Schuster W, Brennicke A: RNA editing in plant mitochondria. Science 246: 1632–1634 (1989).
Hyde SC, Emsley P, Hartshorn MJ, Mimmack MM, Gileadi U, Pearce SR, Gallagher MP, Gill DR, Hubbard RE, Higgins CF: Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resitence and bacterial transport. Nature 346: 362–365 (1990).
Kyte J, Doolittle RF: A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132 (1982).
Lipman DJ, Pearson WR: Rapid and sensitive similarity searches. Science 227: 1435–1441 (1985).
Michaelis G, Vahrenholz C, Pratje E: Mitochondrial DNA of Chlamydomonas reinhardtii: the gene for apocytochrome b and the complete functional map of the 15.8 kb DNA. Mol Gen Genet 223: 211–216 (1990).
Nicholson DW, Neupert W: Import of cytochrome c into mitochondria: reduction of heme, mediated by NADH and flavin nucleotides, is obligatory for its covalent linkage to apocytochrome c. Proc Natl Acad Sci USA 86: 4340–4344 (1989).
Nicholson DW, Stuart RA, Neupert W: Biogenesis of cytochrome c1. J Biol Chem 264: 10156–10168 (1989).
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K: Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. J Mol Biol 223: 1–7 (1992).
Pearson WR, Lipman DJ: Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85: 2444–2448 (1988).
Ramseier TM, Winteler HV, Hennecke H: Discovery and sequence analysis of bacterial genes involved in the biogenesis of c-type cytochromes. J Biol Chem 266: 7793–7803 (1991).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulson A: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Schuster W, Combettes B, Flieger K, Brennicke A: A plant mitochondrial gene encodes a protein involved in cytochrome c biogenesis. Mol Gen Genet 239: 49–57 (1993).
Schuster W, Hiesel R, Isaac PG, Leaver CJ, Brennicke A: Transcript termini of messenger RNAs in higher plant mitochondria. Nucl Acids Res 14: 5943–5954 (1986).
Schuster W, Hiesel R, Wissinger B, Schobel W, Brennicke A: Isolation and analysis of plant mitochondria and their genomes. In: Shaw CH (ed) Plant Molecular Biology, pp. 79–1020. IRL Press, Oxford/Washington DC (1989).
Schuster W, Unseld M, Wissinger B, Brennicke A: Ribosomal protein S14 transcripts are edited in Oenothera mitochondria. Nucl Acids Res 18: 229–233 (1990).
Ward BL, Anderson RS, Bendich AJ: The mitochondrial genome is large and variable in a family of plants (Cu-curbitaceae). Cell 25: 793–803 (1981).
Wissinger B, Schuster W, Brennicke A: Trans-splicing in Oenothera mitochondria: nad1 mRNAs are edited in exon and trans splicing group II intron sequences. Cell 65: 473–482 (1991).
Wissinger B, Brennicke A, Schuster W: Regenerating good sense: RNA editing and trans-splicing in plant mitochondria. Trends Genet 8: 322–328 (1992).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schuster, W. The highly edited orf206 in Oenothera mitochondria may encode a component of a heme transporter involved in cytochrome c biogenesis. Plant Mol Biol 25, 33–42 (1994). https://doi.org/10.1007/BF00024196
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00024196