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Plant Growth Regulation

, Volume 17, Issue 2, pp 157–165 | Cite as

Polyamines, floral induction and floral development of strawberry (Fragaria ananassa Duch.)

  • Elisabeth Tarenghi
  • Josette Martin-Tanguy
Article

Abstract

In the short-day plant, strawberry (Fragaria ananassa Duch.), polyamines (putrescine, spermidine and spermine), conjugated spermidine (water-insoluble compounds) and bound amines (putrescine, spermidine, phenylethylamine, 3-hydroxy, 4-methoxyphenylethylamine) accumulated in the shoot tips during floral induction and before floral emergence. Different associations of free amines and conjugated amines were observed during floral induction, as compared with the reproductive phase. During the whole period of floral development, phenylethylamine (an aromatic amine) was the predominant amine, representing 80 to 90% of the total free amine pool. Phenylethylamine conjugates (water-insoluble compounds) were the predominant amides observed prior to fertilization. These substances decreased drastically after fertilization. In vegetative shoot tips from plants grown continously under long days, free polyamines (putrescine, spermidine) and bound polyamines (putrescine, spermidine) were low and no change was observed. Free amines (spermine and phenylethylamine), bound aromatic amines (phenylethylamine, 3-hydroxy, 4-methoxyphenylethylamine), conjugated spermidine and phenylethylamine did not appear. Male-sterile flowers were distinguished by their lack of conjugated spermidine and phenylethyalamine and by a decrease in free phenylethylamine. In normal and sterile strawberry plants α-DL-difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase (ODC), caused inhibition of flowering and free and polyamine conjugates. When putrescine was added, polyamine titers and flowering were restored. A similar treatment with α-DL-difluoromethylarginine (DFMA), a specific, irreversible inhibitor of arginine decarboxylase (ADC), did not affect flowering and polyamine titers. These results suggest that ornithine decarboxylase (ODC) and polyamines are involved in regulating floral initiation in strawberry. The relationship between polyamines, aromatic amines, conjugates, floral initiation and male sterility is discussed.

Key words

aromatic amines bound amines conjugated amines floral development floral induction male sterility polyamines strawberry (Fragaria ananassa Duch.) 

Abbreviations

ADC

arginine decarboxylase

ODC

ornithine decarboxylase

DFMA

α-DL-difluoromethylarginine

DFMO

α-DL-difluoromethylornithine

Put

putrescine

Spd

spermidine

Spm

spermine

Phen

phenylethylamine

3H4M Phen

3-hydroxy, 4-methoxyphenylethylamine

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Elisabeth Tarenghi
    • 1
  • Josette Martin-Tanguy
    • 1
  1. 1.INRA, Station de Génétique et d'Amélioration des PlantesDijon C'dexFrance

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