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Effects of 1-aminocyclopropane-1-carboxylic acid and oxygen concentrations on in vivo and in vitro activity of ACC oxidase of sunflower hypocotyl segments

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Abstract

In vivo ethylene production by hypocotyl segments of sunflower seedlings and in vitro activity of 1-aminocyclopropane-1-carboxylic acid oxidase (formerly ethylene-forming enzyme) extacted from the same tissues increase with increasing concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC) and oxygen. ACC oxidase activity follows Michaelis-Menten kinetics. The apparent Km values of the enzyme towards ACC, estimated in vivo and in vitro, are respectively 219 μM and 20.6 μM. Both Km values towards O2 are similar, ca 10.6–11.4%. A decrease in concentration in one of the substrates (ACC or O2) results in an increase in in vivo apparent Km of ACC oxidase for the other substrate. On the contrary, Km values of the enzyme towards ACC or O2 estimated in vitro are not dependent upon the concentration of the other substrate (ACC or O2).

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

EFE:

ethylene-forming enzyme

MACC:

malonylate 1-aminocyclopropane-1-carboxylic acid

SD:

standard deviation

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Authors and Affiliations

  1. Physiologie Végétale Appliquée, Université Pierre et Marie Curie, Tour 53, 1 er étage, 4 place Jussieu, 75252, Paris cedex 05, France

    C. Bailly, D. Dumet, F. Corbineau & D. Côme

  2. Institute of Botany, University of Warsaw, Krakowskie Przedmiescie 26/28, 00-927, Warszawa, Poland

    R. Bogatek

Authors
  1. C. Bailly
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  2. R. Bogatek
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  3. D. Dumet
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  4. F. Corbineau
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  5. D. Côme
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Bailly, C., Bogatek, R., Dumet, D. et al. Effects of 1-aminocyclopropane-1-carboxylic acid and oxygen concentrations on in vivo and in vitro activity of ACC oxidase of sunflower hypocotyl segments. Plant Growth Regul 17, 133–139 (1995). https://doi.org/10.1007/BF00024173

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  • DOI: https://doi.org/10.1007/BF00024173

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