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Effect of 2-chloroethyltrimethyl ammonium chloride on tuberization and endogenous GA3 in roots of potato cuttings

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Abstract

Cuttings of potato shoots treated with the plant growth retardant 2-chloroethyltrimethyl ammonium chloride (CCC) form tubers earlier and have less biologically-active gibberellin (GA)-like substances in the roots than control cuttings. The major GA-like substance in roots of potato cuttings was identified as GA3 by gas-chromatography-mass spectrometry (GC-MS). The content of GA3 in roots of control cuttings, estimated by GC-MS-selected ion monitoring (SIM) using [17, 17-2H]GA3 as a quantitative internal standard, was 38.8 ng per g fresh weight (fw), and in roots of CCC-treated cuttings, in which tuberization was promoted, was 0.6 ng per g fw. Gibberellin A1, GA8 and GA20 were also indicated as minor components of roots from both control and CCC-treated cuttings. The comparatively high GA3 content in roots of control cuttings might be the ‘root factor’ responsible for delaying tuberization in potato.

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Abbreviations

CCC:

2-chloroethyltrimethyl ammonium chloride

dw:

dry weight

EtOAc:

ethyl acetate

GA:

gibberellin

GC-MS-SIM:

gas chromatography-mass spectrometry-selected ion monitoring

HPLC:

high performance liquid chromatography

IAA:

indole-3-acetic acid

KRI:

Kovats' retention index

MeOH:

methanol

MeTMSi:

methyl ester trimethylsilyl ether

NAA:

naphthalene acetic acid

SD:

short day(s)

2,4-D:

2,4-dichlorophenoxy acetic acid

References

  1. Dyson PW (1965) Effect of gibberellic acid and (2-chloroethyl)trimethylammonium chloride on potato growth and development. J Sci Food Agric 16: 542–549

    PubMed  Google Scholar 

  2. Ewing EE (1987) The role of hormones in potato (Solanum tuberosum L.) tuberization. In: Davies PJ (ed) Plant Hormones and Their Role in Plant Growth and Development, pp 515–538

  3. Ewing EE and Wareing PF (1978) Shoot, stolon, and tuber formation on potato (Solanum tuberosum L.) cuttings in response to photoperiods. Plant Physiol 61: 348–353

    Google Scholar 

  4. Goleniowski M, de Bottini GA and Tizio R (1980) The effect of CCC [(2-chloroethyl)trimethylammonium chloride] and its interaction with gibberellic acid on quali and quantitative evolution of gibberellin-like substances in potato plants (Solanum tuberosum L.) grown under unfavorable conditions for tuberization. Phyton 38: 13–22

    Google Scholar 

  5. Gregory L (1956) Some factors for tuberization in the potato plant. Amer J Bot 43: 281–288

    Google Scholar 

  6. Guiñazú M, Abdala G and Tizio R (1988) Effect of free and conjugated gibberellins on roots of potato cuttings treated with CCC [(2-chloroethyl)trimethylammonium chloride] in relation to tuber formation. J Plant Physiol 132: 725–730

    Google Scholar 

  7. Harmey MA, Croosley MP and Clinch PEM (1966) The effect of growth regulators on tuberization of cultured stem pieces of Solanum tuberosum. Eur Potato J 9: 146–151

    Google Scholar 

  8. Harvey BMR, Crothers SH, Evans NE and Selby C (1991) The use of growth retardants to improve microtuber formation by potato (Solanum tuberosum). Plant Cell, Tissue and Organ Culture 27: 59–64

    Google Scholar 

  9. Jones MG, Horgan R and Hall MA (1988) Endogenous gibberellins in the potato, Solanum tuberosum. Phytochemistry 27: 7–10

    Article  Google Scholar 

  10. Koda Y and Okazawa Y (1983) Influences of environmental, hormonal and nutritional factors on potato tuberization in vitro. Japan J Crop Sci 42: 582–591

    Google Scholar 

  11. Koda Y and Okazawa Y (1983) Characteristic changes in the levels of endogenous plant hormones in relation to the onset of potato tuberization. Japan J Crop Sci 52: 592–597

    Google Scholar 

  12. Koda Y, Okazawa Y (1988) Detection of potato tuber inducing activity in potato leaves and old tubers. Plant Cell Physiol 29: 969–974

    Google Scholar 

  13. Koda Y, Omer EA, Yoshihara T, Shibata H, Sakamura S and Okazawa Y (1988) Isolation of a specific potato tuber inducing substance from potato leaves. Plant Cell Physiol 29: 1047–1051

    Google Scholar 

  14. Koshioka M, Takeno K, Beall FD and Pharis RP (1983) Purification and separation of plant gibberellins from their precursors and glucosyl conjugates. Plant Physiol 73: 389–406

    Google Scholar 

  15. Langille AR and Hepler PR (1992) Effect of three anti-gibberellin growth retardants on tuberization of induced and non-induced Katahdin potato leaf-bud cuttings. Amer Potato J 69: 131–141

    Google Scholar 

  16. Levy D, Seabrook JA and Coleman S (1993) Enhancement of tuberization of axillary shoot buds of potato (Solanum tuberosum L.) cultivars cultured in vitro. J Exp Botany 44: 381–386

    Google Scholar 

  17. Murakami Y (1968) A new rice seedling bioassay for gibberellins, “Microdrop Method”, and its use for testing of rice and morning glory. Bot Mag 81: 33–43

    Google Scholar 

  18. Nitsch JP (1951) Growth and development of excised ovaries. Am J Bot 38: 566–578

    Google Scholar 

  19. Okazawa Y (1960) Studies on the relation between the tuber formation of potato plant and its natural gibberellin content. Proc Crop Sci Soc Japan 29: 121–124

    Google Scholar 

  20. Okazawa Y (1967) Physiological studies on the tuberization of potato plants. J Fac Agr Hokkaido Univ Sapporo 55: 267–336

    Google Scholar 

  21. Palmer CE and Smith OE (1969) Cytokinins and tuber initiation in the potato. Nature 221: 279–280

    Google Scholar 

  22. Parrot F (1975) Interaction des sels minéraux et de l'acide gibbérellique sur la tubérisation de fragments de tiges de pomme de terre cultivés in vitro. Potato Res 18: 446–450

    Google Scholar 

  23. Pelacho AM and Mingo-Castel AM (1991) Jasmonic acid induces tuberization of potato stolons cultured in vitro. Plant Physiol 97: 1253–1255

    Google Scholar 

  24. Pont-Lezica RF (1970) Evolution des substances de type gibberellines chez la pomme de terre pendant la tubérisation, en relation avec la longueur du jour et la temperature. Potato Res 13: 323–331

    Google Scholar 

  25. Racca RW and Tizio R (1968) A preliminary study of changes in the content of gibberellin-like substances in the potato plant in relation to the tuberization mechanism. Eur Potato J 11: 213–220

    Google Scholar 

  26. Sattelmacher B and Marschner H (1978) Relation between nitrogen, cytokinin activity and tuberization in Solanum tuberosum L. Physiol Plant 44: 65–68

    Google Scholar 

  27. Stallknecht GF (1972) Cumarin induced tuber formation on excised shoots of Solanum tuberosum L. cultured in vitro. Plant Physiol 50: 412–413

    Google Scholar 

  28. Tanimoto E (1991) Gibberellin requirement for the normal growth of roots. In: Takahashi N, Phinney BO, MacMillan J (eds) Gibberellins, pp 229–240. New York: Springer-Verlag

    Google Scholar 

  29. Tizio R (1964) Effet du systeme radiculaire sur la tubérisation de la pomme de terre. C r Acad Sc Paris 258: 6503–6506

    Google Scholar 

  30. Tizio R (1964) Action de l'acide gibbérellique sur la tubérisation de la pomme de terre. C r Acad Sc Paris 259: 1187–1190

    Google Scholar 

  31. Tizio R (1966) Interaction du facteur radiculaire et de l'acide gibbérellique sur la croissance des stolons et la tubérisation de la pomme de terre. C r Acad Sc Paris 262: 767–770

    Google Scholar 

  32. Tizio R (1969) Action du CCC [Chlorure de [(2-chloroethyl)trimethylammonium] sur la tubérisation de la pomme de terre. Eur Potato J 12: 3–7

    Google Scholar 

  33. Tizio R (1971) Action et rôle probable de certaines gibbérellines (A1, A3, A4, A5, A7, A9 et A13) sur la croissance des stolons et la tuberisation de la pomme de terre (Solanum tuberosum L.). Potato Res 14: 193–204

    Google Scholar 

  34. Tizio R (1979) Contribution a l'étude du mécanisme hormonal de tubérisation de la pomme de terre (Solanum tuberosum L.). Thése Doct Etat, Université Pierre et Marie Curie, Paris 6

  35. Tizio R and Goleniowski M (1985) Nouvelles recherches sur la nature gibbérellinique du “facteur radiculaire” qui retarde la tubérisation de germes de pomme de terre cultivés in vitro. C r Acad Sc Paris 300, Série III no 13: 499–502

  36. Vreugdenhil D and Struik PC (1989) An integrated view of the hormonal regulation of tuber formation in potato Solanum tuberosum. Physiol Plant 75: 525–531

    Google Scholar 

  37. Vreugdenhil D, Bindels P, Reinhoud P, Klocek J and Hendriks T (1994) Use of the growth retardant tetcyclasis for potato tuber formation in vitro. Plant Growth Regul 14: 257–265

    Google Scholar 

  38. Yoshihara T, Omer EA, Koshino H, Sakamura S, Kikuta Y and Koda Y (1989) Structure of a tuber-inducing stimulus from potato leaves (Solanum tuberosum L.). Agric Biol Chem 53: 2835–2837

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, 5800, Río Cuarto, Argentina

    Guillermina Abdala

  2. Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, 5505 Chacras de Corias, Mendoza, Argentina

    Mónica Guiñazú & Ricardo Tizio

  3. Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, Alberta, Canada

    David W. Pearce & Richard P. Pharis

Authors
  1. Guillermina Abdala
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  2. Mónica Guiñazú
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  3. Ricardo Tizio
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  4. David W. Pearce
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  5. Richard P. Pharis
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Abdala, G., Guiñazú, M., Tizio, R. et al. Effect of 2-chloroethyltrimethyl ammonium chloride on tuberization and endogenous GA3 in roots of potato cuttings. Plant Growth Regul 17, 95–100 (1995). https://doi.org/10.1007/BF00024167

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  • DOI: https://doi.org/10.1007/BF00024167

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