Summary
The male sterility system MS-1 of Brassica oleracea was studied in order to elucidate if nucleo-cytoplasmic interactions determine this system. Crosses of male sterile MS-1 genotypes with heterozygous MS-5 genotypes gave fully fertile F1 progenies. Selfing of seven F1 plants resulted in five F2 populations showing a 9:7 segregation ratio and two a 3:1 ratio for fertile and male sterile plants. Two F2 progenies deviated from the expected 9:7 or 3:1 segregation ratios for fertile and male sterile plants. Thermosensitivity and distortion of the meiosis are suggested as the causal factors underlying the deviation of the segregation ratios. It was concluded that nuclear factors determine the male sterility in the MS-1 system, because the presence of a nucleocytoplasmic interaction in this system should have given only a 3:1 segregation ratio for fertile and male sterile plants in the F2 generation.
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References
Bannerot H., L.Boulidard, Y.Cauderon & J.Tempe, 1974. Transfer of cytoplasmatic male sterility from Raphanus sativus to Brassica oleracea. Proc. Eucarpia Meet. Cruciferae Crop Section 25: 52–54.
Bonhomme S., F.Budar, M.Férault & G.Pelletier, 1991. A 2.5 kB NcoI fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male-sterility in Brassica cybrids. Curr. Genet. 19: 121–127.
Borchers E.A., 1966. Characteristics of a male sterile mutant in purple cauliflower (Brassica oleracea L.). Proc. Amer. Soc. Hort. Sci. 88: 406–410.
Brown A.P. & A.F.Dyer, 1991. Effects of low temperature storage on the pollen of Brassica campestris, B. oleracea and B. napus. Euphytica 51: 215–218.
Chiang M.S. & R.Crete, 1987. Cytoplasmic male sterility in Brassica oleracea induced by the B. napus cytoplasm—female fertility and restoration of male sterility. Can. J. Plant Sci. 67 (3): 891–897.
Cole K., 1959. Inheritance of male sterility in green sprouting broccoli. Can. J. Genet. Cytol. 1: 203–207.
Chatterjee S.S. & V.Swarup, 1972. Indian cauliflower has still greater future. Indian Hortic. 17: 18–20.
Dickson M.H., 1970. A temperature sensitive male sterile gene in Broccoli, Brassica oleracea L. var. Italica. J. Amer. Soc. Hort. Sci. 95(1): 13–14.
Earle E.D., V.E.Gracen, V.M.Best, L.A.Batts & M.E.Smith, 1987. Fertile revertants from S-type male-sterile maize grown in vitro. Theor. Appl. Genet. 74: 601–609.
Evenor D. & S.Izhar, 1984. Coexistence of cytoplasmic and nuclear genes for male sterility in Petunia. Mol. Gen. Genet. 194: 523–527.
Evenor D., A.Guri, S.Izhar, 1984. The relationship between a nuclear restorer gene and the nuclear gene for male sterility. Theor. Appl. Genet. 67: 475–478.
Gouyon P.-H., F.Vichot & J.M.M.vanDamme, 1991. Nuclear-cytoplasmic male sterility: single-point equilibria versus limit cycles. Am. Nat. 137: 498–514.
Johnson A.G., 1958. Male sterility in Brassica. Nature 182: 1523.
Kaul M.L.H., 1988. Male Sterility in Higher Plants. Springer Verlag, Berlin.
Landry B.S., N.Hubert, R.Crete, M.S.Chiang, S.E.Lincoln & T.Etoh, 1992. A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmadiophora brassicae (Wororin). Genome 35: 409–420.
Nieuwhof M., 1961. Male sterility in some cole crops. Euphytica 10: 351–356.
Nieuwhof M., 1968. Identity tests of Ms genes of Brussels sprouts (Brassica oleracea L. var. gemmifera DC.) and cauliflower (B. oleracea var. botrytis L.). Euphytica 17: 202–206.
Nishi S. & T.Hiraoka, 1958. Studies on F1 hybrid vegetable crops (1) Studies on the utilization of male sterility on F1 seed production I. Histological studies on the degenerative process of male sterility in some vegetable crops. Bull. Natl. Inst. Agric. Sci. Jpn. Ser. E 6: 1–41.
Pearson O.H., 1972. Cytoplasmically inherited male sterility characters and flower components from the species cross Brassica nigra (L) Koch×B. oleracea L. J. Am. Soc. Hort. Sci. 97: 398–402.
Renard M., R.Delourme, J.Mesquida, G.Pelletier, C.Primard, L.Boulidard, C.Dore, V.Ruffio, Y.Herve & J.Morice, 1992. Male sterilities and F1 hybrids in Brassica. In: Y.Dattee, C.Dumas & A.Gallais (Eds.), Reproductive Biology and Plant Breeding, pp. 107–119. Springer Verlag, Berlin.
Rottmann W.H., T.Brears, T.P.Hodge & D.M.Lonsdale, 1987. A mitochondrial gene is lost via homologous recombination during reverion of CMS T maize to fertility. E.M.B.O. Journal 6: 1541–1546.
Rundfeldt H., 1960. Unterzuchungen zur Züchtung des Kopfkohls (B. oleracea L. var. capitata). Z. Pflanzenzücht. 44: 30–62.
Sampson D.R., 1966. Genetic analysis of Brassica oleracea using nine genes from sprouting broccoli. Can. J. Genet. Cytol. 8: 404–413.
Sampson D.R., 1970. Close linkage of genes for male sterility and anthocyanin synthesis in Brassica oleracea promising for F1 hybrid seed production; multivalents at meiosis not involved in the linkage. Can. J. Genet. Cytol. 12: 677–684.
Slocum M.K., S.S.Figdore, W.C.Kennard, J.Y.Suzuki & T.C.Osborn, 1990. Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor. Appl. Genet. 80: 57–64.
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Kik, C., Zaal, M.A.C.M. & Verbeek, W.H.J. Analysis of genic male sterility in Brassica oleracea . Euphytica 68, 53–57 (1993). https://doi.org/10.1007/BF00024154
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DOI: https://doi.org/10.1007/BF00024154