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Changes in abscisic acid and its glucose ester in Phaseolus vulgaris L. during chilling and water stress

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Abstract

Levels of free and conjugated abscisic acid (ABA) were determined in leaves and roots of intact bean (Phaseolus vulgaris L., cv. Mondragone) seedlings under chilling (3ΔC) and drought as well as during recovery from stress. Abscisic acid-glucose ester (ABAGE) was the only conjugate releasing free ABA after alkaline hydrolysis of the crude aqueous extracts. During the first 20–30 h chilled plants rapidly dehydrated and wilted without any change in ABA and ABAGE levels. Subsequently, leaf and root ABA levels increased and plants regained turgor. ABAGE concentration showed a slight increase in leaves but not in roots. Upon recovery from chilling a transient, but significant, rise in leaf ABA content was observed, while no appreciable change in ABAGE was found. Drought triggered ABA accumulation in leaves and roots, while a rise in ABAGE content was detected only in leaf tissues. Recovery from stress caused a drop in ABA levels without a correspondent increase in ABAGE concentration. We conclude that ABAGE is not a source of free ABA during either chilling or water stress and that only a small proportion of the ABA produced under stress is metabolised to ABAGE during recovery.

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Abbreviations

ABA =:

abscisic acid

ABAGE =:

abscisic acid-glucose ester

DW =:

dry weight

FW =:

fresh weight

RIA =:

radioimmunoassay

RWC =:

relative water content

Ψw =:

water potential

Ψo =:

osmotic potential

Ψp =:

turgor potential

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Authors and Affiliations

  1. Dipartimento di Biologia delle Piante Agrarie, Sezione di Orticoltura e Floricoltura, viale delle Piagge 23, 56124, Pisa, Italy

    Paolo Vernieri, Alberto Pardossi & Franco Tognoni

  2. Scuola Superiore Studi Universitari e Perfezionamento ‘S.Anna’, via Carducci 40, 56100, Pisa, Italy

    Giovanni Serra

Authors
  1. Paolo Vernieri
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  2. Alberto Pardossi
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  3. Giovanni Serra
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  4. Franco Tognoni
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Vernieri, P., Pardossi, A., Serra, G. et al. Changes in abscisic acid and its glucose ester in Phaseolus vulgaris L. during chilling and water stress. Plant Growth Regul 15, 157–163 (1994). https://doi.org/10.1007/BF00024105

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  • DOI: https://doi.org/10.1007/BF00024105

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