Advertisement

Plant Growth Regulation

, Volume 11, Issue 2, pp 165–171 | Cite as

Effect of triacontanol and chlormequat on growth, plant hormones and artemisinin yield in Artemisia annua L.

  • A. Shukla
  • A. H. Abad Farooqi
  • Y. N. Shukla
  • S. Sharma
Regular Paper

Abstract

Artemisinin and herbage yield of Artemisia annua plants were determined after application of triacontanol (tria.) and chlormequat (2-chloroethyltrimethylammonium chloride). Tria. at 1.0 and 1.5 mgl−1 produced a statistically significant positive effect on artemisinin level as well as on plant height, leaf and herbage yield. Chlormequat at 1000 and 1500 mgl−1 also increased artemisinin level, decreased the plant height at higher concentrations and increased the leaf and herbage yield at lower concentrations. Tria. application enhanced GA-like activity, but ABA levels decreased, while chlormequat increased ABA but reduced GA-like substances. The effect of Tria. on artemisinin yield seems to be mediated through its effect on plant growth.

Key words

Artemisinin chlormequat endogenous plant hormones triacontanol Artemisia annua L. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Charles DJ and Simon JE (1990) Germplasm variation in artemisinin content of Artemisia annua using alternative method of artemisinin analysis from crude plant extract. J Nat Products 53: 157–160Google Scholar
  2. 2.
    Chen FT and Zhang GH (1987) Studies on several physiological factors on artemisinin synthesis in Artemisia annua L. Plant Physiol Commun 5: 26–30Google Scholar
  3. 3.
    El-Keltawi NE and Crotaeu R (1987) Influence of foliar applied cytokinins on growth and essential oil content of several members of lamiaceae. Phytochemistry 26: 891–895CrossRefGoogle Scholar
  4. 4.
    Evans ML (1984) Functions of hormones at the cellular level of organization. In: TKScott, ed. Encyclopedia of Plant Physiology (New Series), Vol. 10, p 30. Berlin-Heidelberg-New York: Springer-VerlagGoogle Scholar
  5. 5.
    Farooqi Abad AH and Sharma S (1988) Effect of growth retardants on growth and essential oil content in Japanese mint. Plant Grow Regul 7: 39–45Google Scholar
  6. 6.
    Farooqi Abad AH, Shukla YN, Sharma S and Bangerth F (1989) Endogenous inhibitors and seasonal changes in abscisic acid in Dioscorea floribunda Mart and Gal. Plant Grow Regul 8: 225–232Google Scholar
  7. 7.
    Hangarter R, Ries SK and Carbon P (1978) Effects of triacontanol on plant cell cultures in vitro. Plant Physiol 61: 855–858Google Scholar
  8. 8.
    Hedden P (1990) The action of plant growth retardants at the biochemical level. In: RPPharis and SBRood, eds. Plant Growth Substances 1988, pp. 322–332, Berlin-Heidelberg-New York: Springer-VerlagGoogle Scholar
  9. 9.
    Houtz RL, Ries SK and Tolbert NE (1985) Effect of triacontanol on Chlamdomonas. Plant Physiol 79: 357–364Google Scholar
  10. 10.
    Jayasrce SS and Thomas J (1990) Oil yield and quality of lemongrass as influenced by growth regulator under different planting methods. Int J Trop Agric 8: 27–28Google Scholar
  11. 11.
    Klayman DL (1985) Qinghaosu—An antimalarial drug from China. Science 228: 1049–1055PubMedGoogle Scholar
  12. 12.
    Kudakasseril CJ, Lam L and Staba EJ (1987) Effect of sterol inhibitors on the incorporation of C14-IPP into artemisinin by a cell free system from Artemisia annua tissue cultures and plants. Planta Med 53: 280–284Google Scholar
  13. 13.
    Liersch R, Soicke H, Stechr C and Tullner HV (1986) Formation of artemisinin in Artemisia annua during one vegetation period. Planta Med 52: 387–390Google Scholar
  14. 14.
    Mathysse AG and Scott TK (1984) Functions of hormones at the whole plant level of organization. In: APirson and MHZimmermann, eds. Encyclopedia of Plant Physiology (New series), Vol. 10, pp. 219–243. Berlin-Heidelberg-New York: Springer-VerlagGoogle Scholar
  15. 15.
    Misra A and Srivastava NK (1991) Effect of triacontanol formulation ‘Miraculan’ on photosynthesis, growth, nutrient uptake, and essential oil yield of lemongrass (Cymbopogon flexuosus) Steud. watts. Plant Grow Regul 10: 57–63Google Scholar
  16. 16.
    Murakami Y (1968) The microdrop method, a new rice seedling test for gibberellins and its use for testing extracts of rice and morning glory. Bot Mag Tokyo 79: 33–43Google Scholar
  17. 17.
    Nickle LG (1982) Plant Growth Regulators—Agricultural uses, p. 37. Berlin-Heidelberg-New York: Springer-VerlagGoogle Scholar
  18. 18.
    Phillipson JD and O'Neill MJ (1987) Antimalarial and amoebicidal natural products. In: KHostettmann and PJLea, eds. Biologically active natural products, pp. 49–57, Oxford: Oxford University PressGoogle Scholar
  19. 19.
    Ramani S and Kannan S (1980) Effect of triacontanol on absorption and transport of Rb+ and PO4 in plants. Z. Pflanzenphysiol 99: 427–433Google Scholar
  20. 20.
    Ries SK and Wert V (1977) Growth response of rice seedling to triacontanol in light and dark. Planta 135: 77–82Google Scholar
  21. 21.
    Ries S, Wert V, O'Leary NFD and Nair M (1990) 9-β-L (+) Adenosine: A new naturally occurring plant growth substance elicited by triacontanol in rice. Plant Grow Regul 9: 263–273Google Scholar
  22. 22.
    Ries SK, Wert V, Sweeley CC and Leavitt RA (1977) Triacontanol: A new naturally occurring plant growth regulator. Science 195: 1339–1341Google Scholar
  23. 23.
    Shukla A and Farooqi Abad AH (1990) Utilization of plant growth regulators in aromatic plant production. Curr Res Med Arom Plants 12: 152–157Google Scholar
  24. 24.
    Shukla A, Farooqi Abad AH and Shukla YN (1991) Growth inhibitors from Artemisia annua. Indian Drugs 28: 376–377Google Scholar
  25. 25.
    Shukla A, Farooqi Abad AH and Shukla YN (1991) Cytokinins from Artemisia annua. Plant Grow Regul (communicated)Google Scholar
  26. 26.
    Singh A, Kaul VK, Mahajan VP, Singh A, Misra LN, Thakur RS and Husain A (1986) Introduction of Artemisia annua in India and isolation of artemisinin, a promising antimalarial drug. Indian J Pharm Sci 48: 137–138Google Scholar
  27. 27.
    Singh A, Vishwakarma RA and Husain A (1988) Evaluation of Artemisia annua strains for higher artemisinin production. Planta Med 54: 475–476Google Scholar
  28. 28.
    Singh N, Luthra R and Sangwan RS (1990) Oxidative pathway of essential oil biosynthesis in the developing Cymbopogon flexuosus leaf. Plant Physiol Biochem 28: 703–710Google Scholar
  29. 29.
    Setia RC, Setia RN, Ahuja KL and Malik CP (1989) Effect of ‘Mixtalol’ on growth yield and yield components of Indian Mustard (Brassica juncea) Plant Grow Regul 8: 185–190Google Scholar
  30. 30.
    Sjut V and Bangerth F (1981) Effect of pollination on treatment with growth regulators on levels of extractable hormones in tomato ovaries and young fruits. Physiol Plant 53: 76–78Google Scholar
  31. 31.
    Srivastava NK and Sharma S (1990) Effect of triacontanol on photosynthesis, alkaloid content and growth in opium poppy (Papaver somniferum). Plant Grow Regul 9: 65–71Google Scholar
  32. 32.
    Theoharides AD, Smyth MH, Ashmore RW, Halverson JM, Zhou ZM, Ridder WE and Ling AJ (1988) Determination of dihydroquinghaosu (DQHS) in blood by pyrolysis GC/MS. Anal. Chem 60: 115–120PubMedGoogle Scholar
  33. 33.
    Ueda J and Kato J (1980) Isolation and identification of a senescence promoting substance from Artemisia absinthium. Plant Physiol 66: 246–249Google Scholar
  34. 34.
    Ueda J, Yamaguchi YY, Takahashi N and Kato J (1987) Identification of gibberellin A3 and abscisic acid from Artemisia capillaris leaves. Agric Biol Chem 51: 595–596Google Scholar
  35. 35.
    Ueda J, Yokota J, Takahashi N, Yoshida M and Kato J (1986) A root promoting factor, capillarol from Artemisia capillaris. Agric Biol Chem 50: 3083–3086Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • A. Shukla
    • 1
  • A. H. Abad Farooqi
    • 1
  • Y. N. Shukla
    • 1
  • S. Sharma
    • 1
  1. 1.Central Institute of Medicinal and Aromatic PlantsLucknowIndia

Personalised recommendations