Skip to main content
SpringerLink
Log in

Stimulation of growth and phospholipase A2 by the peptides mastoparan and melittin and by the auxin 2,4-dichlorophenoxyacetic acid

  • Regular Paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The peptide mastoparan from wasp venom and the peptide melittin from bee venom stimulated growth in etiolated zucchini (Cucurbita pepo L.) hypocotyls. Both peptides were only effective in hypocotyls with abraded cuticles. At concentrations of 2 μg ml−1 peptide growth was stimulated 72% by mastoparan and 50% by melittin after 2 h as compared to the controls. Mastoparan (5 μg ml−1), melittin (10 μg l−1) and 2,4-dichlorophenoxyacetic acid (5×10−4 M) stimulated accumulation of 14C-choline-labeled lysophosphatidylcholine in less than 10 min in cultured soybean cells (Glycine max L.), all to about the same extent. The effects of these peptides are among the first to be reported on plant cells and may be related to important events coupled to growth stimulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

References

  1. André B and Scherer GFE (1991) Stimulation of phospholipase A in membrane vesicles from an auxin-sensitive tissue is mediated by an auxin receptor. Planta 185: 209–214

    Article  Google Scholar 

  2. Argiolas A and Pisano JJ (1983) Facilitation of phospholipase A2 activity by mastoparans, a new class of mast cell degranulating peptides from wasp venom. J Biol Chem 258: 13697–13702

    PubMed  Google Scholar 

  3. Blum W, Hinsch K-D, Schultz G and Weiler EW (1988) Identification of GTP-binding proteins in the plasma membrane of higher plants. Biochem Biophys Res Commun 156: 954–959

    PubMed  Google Scholar 

  4. Brooks RC, McCarthy KD, Lapetina EG and Morell P (1989) Receptor-stimulated phospholipase A2 activation is coupled to influx of external calcium and not to mobilization of intracellular calcium in C62B glioma cells. J Biol Chem 264: 20147–20153

    PubMed  Google Scholar 

  5. Burch RM, Connor JR and Axelrod J (1988) Interleukin amplifies receptor-mediated activation of phospholipase A2 in 3T3 cells. Proc Natl Acad Sci USA 85: 6306–6309

    PubMed  Google Scholar 

  6. Burch RM, Luini A and Axelrod J (1986) Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to a-adrenergic stimulation of FRTL5 thyroid cells. Proc Natl Acad Sci USA 83: 7201–7205

    PubMed  Google Scholar 

  7. Chang J, Musser JH and McGregor H (1987) Phospholipase A2: function and pharmacological regulation. Biochem Pharmacol 15: 2429–2436

    Article  Google Scholar 

  8. Droeback BK, Allan EF, Comerford JG, Roberts K and Dawson P (1988) Presence of a guanine nucleotide-binding protein in a plant hypocotyl microsomal fraction. Biochem Biophys Res Commun 150: 899–902

    PubMed  Google Scholar 

  9. Higashijima T, Burnier J and Ross EM (1990) Regulation of Gi and Go by mastoparan, related amphiphilic peptides and hydrophobic amines. Mechanism and structural determinants of activity. J Biol Chem 265: 14176–14186

    PubMed  Google Scholar 

  10. Higashijima T, Uzu S, Nakajima T and Ross EM (1988) Mastoparan, a peptide toxin from wasp venom, mimicks receptors by activating GTP-binding regulatory proteins (G proteins). J Biol Chem 263: 6491–6494

    PubMed  Google Scholar 

  11. Jacobs M, Thelen MP, Farndale MD, Astle MC and Rubery PH (1988) Specific guanine nucleotide binding by membranes from Cucurbita pepo seedlings. Biochem Biophys Res Commun 155: 1478–1484

    PubMed  Google Scholar 

  12. Jelsema CA (1987) Light-induced changes of phospholipase A2 in rod outer segments and its modulation by GTP-binding proteins. J Biol Chem 262: 163–168

    PubMed  Google Scholar 

  13. Kutschera U, Bergfeld R and Schopfer P (1987) Cooperation of epidermis and inner tissues in auxin-mediated growth of maize coleoptiles. Planta 170: 168–180

    Google Scholar 

  14. Lüthen H, Bigdon M and Böttger M (1990) Reexamination of the acid growth theory of auxin action. Plant Physiol 93: 931–939

    Google Scholar 

  15. Ma H, Yanowsky MF and Meyerowitz EM (1990) Molecular cloning and characterization of GAP1, a G protein α subunit gene from Arabidopsis thaliana. Proc Natl Acad Sci USA 87: 3821–3825

    PubMed  Google Scholar 

  16. Mollay C and Kreil G (1974) Enhancement of bee venom phospholipase A2 activity by melittin, direct lytic factor from cobra venom and polymyxin B. FEBS Lett 46: 141–144

    Article  PubMed  Google Scholar 

  17. Murayama T, Kajiyama Y and Nomura Y (1990) Histamine-stimulated and GTP-binding protein-mediated phospholipase A2 activation in rabbit platelets. J Biol Chem 265: 4290–4295

    PubMed  Google Scholar 

  18. Nickel R, Schütte M, Hecker D and Scherer GFE (1991) The phospholipid platelet-activating factor stimulates proton extrusion in cultured soybean cells and protein phosphorylation and ATPase activity in plasma membranes. J Plant Physiol (in press)

  19. Scherer GFE (1990) Phospholipid-activated protein kinase in plants: coupled to phospholipase A2? In: R.Ranjeva and A.M.Boudet (eds.) Signal Perception and Transduction in Higher Plants, NATO-ASI Series H, vol 47, 69–82, Springer, Berlin, Heidelberg, New York

    Google Scholar 

  20. Scherer GFE and André B (1989) A rapid response to a plant hormone: auxin stimulates phospholipase A2 in vivo and in vitro. Biochem Biophys Res Commun 163: 111–117

    PubMed  Google Scholar 

  21. Scherer GFE and André B (1991) Activation of phospholipase A2 (PLA2) as primary action of auxin: participation of receptor and G protein(s). 14th International Conference on Plant Growth Substances, Amsterdam, Abstract TU-S2-P12, p 46

  22. Scherer GFE, André B and Martiny-Baron G (1990) Hormone-activated phospholipase A2 and lysophospholipid-activated protein kinase: a new signal transduction chain and a new second messenger system in plants? In: (eds Randall DD, Blevins D) Current Topics in Plant Biochemistry and Physiology, University of Columbia-Missouri, vol 9, 190–218

  23. Silk ST, Clejan S and Witkom K (1989) Evidence of GTP-binding protein regulation of phospholipase A2 activity in isolated human platelet membranes. J Biol Chem 264: 21466–21469

    PubMed  Google Scholar 

  24. Teitelbaum I (1990) The epidermal growth factor receptor is coupled to the phospholipase A2-specific pertussis toxin-inhibitable guanine nucleotide-binding regulatory protein in cultured inner medullary collecting tubule cells. J Biol Chem 265: 4218–4222

    PubMed  Google Scholar 

  25. Tillmann U, Viola G, Kayser B, Siemeister G, Hesse T, Palme K, Löbler M and Klämbt D (1989) cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods. EMBO J 8: 2463–2467

    PubMed  Google Scholar 

  26. Zbell B, Hohenadel H, Schwendemann I and Walter-Back C (1990) Is a GTP-binding protein involved in the auxin-mediated phosphoinositide response on plant cell membranes? In: (Konijn TM, ed) Activation and Desensitization of Transducing Pathways. NATO ASI Series, Springer, Berlin Heidelberg New York Tokyo (in press)

    Google Scholar 

  27. Zbell B, Paulik M and Morré DJ (1990) Comparison of 35S-GTP S binding to plasma membranes and endomembranes prepared from soybean and rat. Protoplasma 154: 74–79

    Google Scholar 

  28. Zbell B, Schwendemann I and Bopp M (1989) High-affinity GTP-binding on microsomal membranes prepared from moss protonema of Funaria hygrometrica. J Plant Physiol 134: 639–641

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botanisches Institut, Universität Bonn, Venusbergweg 22, D-5300, Bonn 1, Germany

    G. F. E. Scherer

Authors
  1. G. F. E. Scherer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scherer, G.F.E. Stimulation of growth and phospholipase A2 by the peptides mastoparan and melittin and by the auxin 2,4-dichlorophenoxyacetic acid. Plant Growth Regul 11, 153–157 (1992). https://doi.org/10.1007/BF00024069

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00024069

Key words

Search

Navigation