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Application of AFLP™-based molecular markers to breeding of Populus spp.

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Abstract

Molecular marker technologies have eased and potentiated genetic analysis of plants and have become an extremely useful tool in forest tree breeding. The information provided by molecular markers has made it possible to acquire further knowledge about the structure and organization of plant genomes as well as about the evolution of these plant genomes through phylogenetic analysis. Using Populus spp. as a model tree, this paper aims at showing and discussing the possible applications of AFLP™, a high-density DNA marker technology developed by Keygene N.V. (Wageningen, The Netherlands). Applications include: (i) AFLP analysis of the disease resistance against Melampsora larici-populina using bulked-segregant analysis, (ii) AFLP fingerprinting for identification and taxonomic analysis of individual trees, and (iii) AFLP-based mapping strategies in Populus.

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Abbreviations

AFLP:

amplified fragment length polymorphism

RFLP:

restriction fragment length polymorphism

PCR:

polymerase chain reaction

QTL:

quantitative trait loci

RAPD:

random amplified polymorphic DNA

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Authors and Affiliations

  1. Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium

    María Teresa Cervera, Jaqueline Gusmão, Ann Van Gysel, Marc Van Montagu & Wout Boerjan

  2. Instituut voor Bosbouw en Wildbeheer, B-9500, Geraardsbergen, Belgium

    Marijke Steenackers

Authors
  1. María Teresa Cervera
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  2. Jaqueline Gusmão
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  3. Marijke Steenackers
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  4. Ann Van Gysel
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  5. Marc Van Montagu
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  6. Wout Boerjan
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Cervera, M.T., Gusmão, J., Steenackers, M. et al. Application of AFLP™-based molecular markers to breeding of Populus spp.. Plant Growth Regul 20, 47–52 (1996). https://doi.org/10.1007/BF00024057

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