Abstract
Shoot tips of in vitro grown plantlets of Rosa multiflora were cryopreserved using an encapsulation/dehydration procedure. The influence of sucrose and silica gel pretreatments on pre- and post-freeze shoot growth were examined. Shoot tips recovered from liquid nitrogen only grew after 24h pretreatment in medium containing 0.5 M sucrose, followed by 2 h drying with silica gel and rapid freezing.
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Abbreviations
- RSC1:
-
modified Murashige and Skoog medium for Rosa multiflora shoot culture
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Lynch, P.T., Harris, W.C. & Chartier-Hollis, J.M. The cryopreservation of shoot tips of Rosa multiflora . Plant Growth Regul 20, 43–45 (1996). https://doi.org/10.1007/BF00024056
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DOI: https://doi.org/10.1007/BF00024056