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The influence of culture vessel head-space volatiles on somatic embryo maturation in Sitka spruce [Picea sitchensis (Bong.) Carr.]

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Abstract

The maturation of somatic embryos of Sitka spruce [Picea sitchensis (Bong.) Carr.] was found to be highly dependent on the method used to seal plastic Petri dishes. Large numbers of well-formed mature embryos developed if dishes were sealed with PVC cling-film (CF) whilst sealing with Parafilm M (PF) greatly reduced the numbers of embryos forming. Inclusion of potassium permanganate oxidation traps, normally used to deplete the atmospheric ethylene, greatly stimulated somatic embryo maturation under PF sealing. Similarly, traps of adsorption agents (Tenax, activated charcoal or soft white paraffin), capable of removing volatiles from the culture vessel head-space, stimulated somatic embryo maturation under PF sealing although to a lesser extent than the oxidation traps. Incorporation of silver nitrate or 2-chloroethylphosphonic acid (ethephon) in the culture medium indicated that ethylene was not the agent supressing somatic embryo maturation under PF sealing.

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Abbreviations

ABA:

abscisic acid

CF:

PVC cling-film

PF:

Parafilm M

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Authors and Affiliations

  1. Applied Plant Science Division, Newforge Lane, BT9 5PX, Belfast, UK

    Chris Selby & Barbara M. R. Harvey

  2. Food Science Division, Department of Agriculture for Northern Ireland, Newforge Lane, BT9 5PX, Belfast, UK

    W. Colin McRoberts & John T. G. Hamilton

  3. Department of Applied Plant Science, The Queen's University of Belfast, Newforge Lane, BT9 5PX, Belfast, UK

    Chris Selby & Barbara M. R. Harvey

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  1. Chris Selby
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  2. W. Colin McRoberts
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  3. John T. G. Hamilton
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  4. Barbara M. R. Harvey
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Selby, C., McRoberts, W.C., Hamilton, J.T.G. et al. The influence of culture vessel head-space volatiles on somatic embryo maturation in Sitka spruce [Picea sitchensis (Bong.) Carr.]. Plant Growth Regul 20, 37–42 (1996). https://doi.org/10.1007/BF00024055

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