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The phenotypic characterisation of R2 generation transgenic rice plants under field and glasshouse conditions

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The phenotypes of seed progeny (R2 generation) of Oryza sativa L. cv. Taipei 309, which carried the neomycin phosphotransferase II (npt II) gene, were compared with those of non-transformed, protoplast-derived plants of the same generation and non-transformed, seed-derived plants under field and glasshouse conditions. Under both conditions the transgenic plants were generally smaller, took longer to flower and had reduced fertility. Significant differences were observed between individuals within the group of transgenic plants. The npt II gene was present in most of the transgenic plants, but NPT II activity was only detected in a minority of individuals.

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References

  • Abdullah R., J.A. Thompson & E.C. Cocking, 1986. Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technol. 4: 1087–1090.

    Article  Google Scholar 

  • Abdullah R., J.A. Thompson, G.S. Kush, R.P. Kaushik & E.C. Cocking, 1989. Protoclonal variation in the seed progeny of plants regenerated from rice protoplasts. Plant Sci. 65: 97–101.

    Article  Google Scholar 

  • Davey M.R., S.L. Kothari, H.M. Zhang, E.L. Rech, E.C. Cocking & P.T. Lynch, 1991. Transgenic rice: Characterisation of protoplast-derived plants and their seed progeny. J. Exp. Bot. 42: 1159–1169.

    Article  CAS  Google Scholar 

  • Doyle J.F. & J.L. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

    Google Scholar 

  • Edwards K., C. Johnstone & C. Thompson, 1991. A simple and rapid method for the preparation of genomic DNA for PCR analysis. Nucleic Acids Res. 19: 1349.

    Article  PubMed  CAS  Google Scholar 

  • Erampalli D., D. Patton, L. Castle, L. Mickelson, K. Hanson, J. Schnall, K.A. Feldmann & D. Meinke, 1991. Embryonic lethals and T-DNA insertional mutagenesis in Arabidopsis. The Plant Cell 3: 149–157.

    Article  Google Scholar 

  • Hayashimoto A., Z. Li & N. Murai, 1990. A polyethylene glycolmediated protoplast transformation system for the production of fertile transgenic rice plants. Plant Physiol. 93: 857–863.

    Article  PubMed  CAS  Google Scholar 

  • Kothari S.L., M.R. Davey, P.T. Lynch, R.P. Finch & E.C. Cocking, 1993. Transgenic rice. In: S-d. Kung & R. Wu (Eds). Transgenic Plants, Volume II, pp. 3–20. Butterworths, New York, London.

    Google Scholar 

  • Luehrsen K.R. & V. Walbot, 1990. Insertion of Mul elements in the first intron of the Adh 1-S gene of maize results in novel RNA processing events. The Plant Cell 2: 1225–1238.

    Article  PubMed  CAS  Google Scholar 

  • Lynch P.T., J. Jones, H. Zang, E.L. Rech, P.S. Eyles, S.L. Kothari, N.W. Blackhall, E.C. Cocking & M.R. Davey, 1992. Transgenic rice plants: Characterisation of two generations of seed progeny. Physiol. Plant. 85: 362–366.

    Article  CAS  Google Scholar 

  • Peng J.-Y., H. Kononowicz & T.K. Hodges, 1992. Transgenic Indica rice plants. Theor. Appl. Genet. 83: 855–863.

    Article  CAS  Google Scholar 

  • Santos I. & R. Salema, 1989. Penicillins and activity of nitrogen metabolism enzymes in plant tissue cultures. Plant Sci. 59: 119–125.

    Article  CAS  Google Scholar 

  • Scowcroft W.R. & P.J. Larkin, 1988. Somaclonal variation. In: G. Block & J. Marsh (Eds). Application of Plant Cell and Tissue Culture, Ciba Foundation Symposium 137, pp. 21–35. Wiley, Chichester.

    Chapter  Google Scholar 

  • Schuh W., M.R. Nelson, D.M. Bigelow, T.V. Orum, C.E. Orth, P.T. Lynch, P.S. Eyles, N.W. Blackhall, J. Jones, E.C. Cocking & M.R. Davey, 1993. The phenotypic characterisation of R2 generation transgenic rice plants under field conditions. Plant Sci. 98: 69–79.

    Article  Google Scholar 

  • Stafford A., 1991. Genetics of cultured plant cells. In: A. Stafford & G. Warren (Eds). Plant Cell and Tissue Culture, pp. 25–47. Open University Press, Milton Keynes, UK.

    Google Scholar 

  • Van Lijsebetens M., R. Vanderhaeghen & M.Van Montagu, 1991. Insertional mutagenesis in Arabidopsis thaliana: isolation of a T-DNA-linked mutation that alters leaf morphology. Theor. Appl. Genet. 81: 277–284.

    Article  Google Scholar 

  • Yang H., H.M. Zhang, M.R. Davey, B.J. Mulligan & E.C. Cocking, 1988. Production of kanamycin-resistant rice tissues following DNA uptake into protoplasts. Plant Cell Rep. 7: 421–425.

    CAS  Google Scholar 

  • Xie Q.J., M.C. Rush & J. Cao, 1990. Somaclonal variation for disease resistance in rice (Oryza sativa L.). In: B.T. Grayson, M.B. Green & L.G. Copping (Eds). Pest Management in Rice, pp. 491–509. Elsevier Applied Science, London.

    Google Scholar 

  • Zhang H.M., H. Yang, E.L. Rech, T.J. Golds, A.S. Davis, B.J. Mulligan, E.C. Cocking & M.R. Davey, 1988. Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts. Plant Cell Reps. 7: 379–384.

    CAS  Google Scholar 

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Lynch, P.T., Jones, J., Blackhall, N.W. et al. The phenotypic characterisation of R2 generation transgenic rice plants under field and glasshouse conditions. Euphytica 85, 395–401 (1995). https://doi.org/10.1007/BF00023972

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