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Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat

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Summary

Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated.

The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.

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Abbreviations

FISH:

fluorescent in situ hybridization

GISH:

genomic in situ hybridization

PRINS:

primer-induced in situ hybridization

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Author notes

  1. S. Abbo

    Present address: Department of Plant Genetics, The Weizmann Institute of Science, 76-100, Rehovot, Israel

  2. I. P. King

    Present address: Deparment of Agricultural Botany, The University of Reading, Whiteknights, P.O. Box 221, RG6 2AS, Reading, U.K.

Authors and Affiliations

  1. John Innes Centre, Colney, NR4 7UH, Norwich, U.K.

    T. E. Miller, S. M. Reader, K. A. Purdie, S. Abbo, R. P. Dunford & I. P. King

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  1. T. E. Miller
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  2. S. M. Reader
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  3. K. A. Purdie
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  4. S. Abbo
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  5. R. P. Dunford
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  6. I. P. King
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Miller, T.E., Reader, S.M., Purdie, K.A. et al. Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat. Euphytica 85, 275–279 (1995). https://doi.org/10.1007/BF00023956

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