Summary
Extensive pollinations were made in attempts to produce selected intergeneric or interspecific hybrids using Nicotiana and Petunia species that exhibit potential for somatic hybridization. Pollination between pairs of species was done by standard and bud-pollination methods. Nicotiana alata or N. tabacum when pollinated reciprocally with selected Petunia species failed to produce hybrids. Likewise, no interspecific hybrids were obtained between Petunia parviflora and 4 Petunia species or P. hybrida. The type of incongruity existing between these species is discussed in relation to the production of hybrids by standard In vitro techniques as compared to somatic hybridization.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brewbaker J. L. & S. K.Majumder, 1961. Cultural studies of the pollen population effect and the self-incompatibility inhibition. Amer. J. Bot. 48: 457–464.
Carlson P. S., H. H.Smith & R. D.Dearing, 1972. Parasexual interspecific plant hybridization. Proc. Natl. Acad. Sci. 69: 2292–2294.
East E. M., 1928. The genetics of the genus Nicotiana. Bibliogr. Genetica 4: 243–320.
Gilissen L. J. W., 1976. The role of the style as a sense organ in relation to wilting of the flower. Planta 131: 201–202.
Gleba Y. Y., R. G.Butenko & K. M.Sytnik, 1975. Fusion of protoplasts and parasexual hybridization in Nicotiana tabacum. Dokl. Akad. Nauk. SSSR 221: 1190–1198.
Kostoff D., 1941. Cytogenetics of the genus Nicotiana. States Printing House, Sofia.
Melchers G. & G.Labib, 1974. Somatic hybridization of plants by fusion of protoplasts. I. Selection of eight resistant hybrids of ‘haploid’ light sensitive varieties of tobacco. Molec. Gen. Genet. 135: 277–294.
North C., 1976. In vitro culture of plant materials as an aid to hybridization. In: K. C.Sink (Ed.), First International Symposium on Floriculture Plant Breeding and Genetics. I.S.H.S., The Hague, Netherlands.
Pogliaga H. H., 1952. Hibrido intergenerico Nicotiana x Petunia. Rev. Argent. Agron. 19: 171–178.
Power J. B. et al., 1976. Somatic hybridization of Petunia hybrida and Petunia parodii. Nature 263: 500–502.
Power J. B. & E. C.Cocking, 1977. Selection systems for somatic hybrids. In: J.Reinert & Y. P. S.Bajaj (Eds), Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer-Verlag, Berlin.
Rangaswamy N. S., 1977. Applications of in vitro pollination and in vitro fertilization. In: J.Reinert & Y. P. S.Bajaj (Eds), Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer-Verlag, Berlin.
Savelli R., 1928–29. Anomalie di germinazione e anomalie delle plantule mei prodotti di Nicotiana rustica e Petunia hybrida. Nuovo Giorn. Botan. Italiano 35: 28–37.
Takebe I., G.Labib & G.Melchers, 1971. Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwiss. 58: 318–320.
Author information
Authors and Affiliations
Additional information
On leave from the Department of Horticulture, Michigan State University, E. Lansing, Michigan, USA. supported by a European Molecular Biology Organization (EMBO) fellowship and The Fred C. Gloeckner Foundation, N. Y., USA.
Supported by the Agricultural Research Council.
Rights and permissions
About this article
Cite this article
Sink, K.C., Power, J.B. Incongruity of interspecific and intergeneric crosses involving Nicotiana and Petunia species that exhibit potential for somatic hybridization. Euphytica 27, 725–730 (1978). https://doi.org/10.1007/BF00023708
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00023708