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A homologue of the 65 kDa regulatory subunit of protein phosphatase 2A in early pea (Pisum sativum L.) embryos

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Abstract

A partial cDNA, isolated from an early developing pea (Pisum sativum L.) embryo library, was found to encode a plant homologue of the regulatory subunit (PR65) of protein phosphatase 2A (PP2A). Comparison of the deduced amino acid sequence with a human PR65 sequence showed that the regulatory subunit of PP2A has been highly conserved during evolution. Southern analysis demonstrated that in pea and rape the catalytic and regulatory subunits of PP2A are encoded by multigene families. The levels of the transcripts encoding each subunit are developmentally regulated during pea embryogenesis and expression of the regulatory subunit is not solely restricted to the embryo.

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References

  1. Arino J, Perez-Callejon E, Cunillera N, Camps M, Posas F, Ferrer A. Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana. Plant Mol Biol 21: 475–485 (1993).

    PubMed  Google Scholar 

  2. Aviv H, Leder P: Purification of biologically active globin mRNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci USA 69: 1408–1412 (1972).

    PubMed  Google Scholar 

  3. Carter PJ, Nimmo HG, Femson CA, Wilkins MB: Bryophyllum fedtschenkoi protein phosphatase type-2A can dephosphorylate phosphoenolpyruvate carboxylase. FEBS Lett 263: 233–236 (1990).

    Article  Google Scholar 

  4. Cohen P: The structure and regulation of protein phosphatases. Annu Rev Biochem 58: 453–508 (1989).

    Article  PubMed  Google Scholar 

  5. Cohen P, Cohen PTW: Protein phosphatases come of age. J Biol Chem 36: 21435–21438 (1989).

    Google Scholar 

  6. Cohen PTW, Brewis ND, Hughes V, Mann DJ: Protein serine/threonine phosphatases; an expanding family. FEBS Lett 268: 355–359 (1990).

    Article  PubMed  Google Scholar 

  7. Evans IM, Swinhoe R, Gatehouse LN, Gatehouse JA, Boulter D: Distribution of root mRNA species in other vegetative organs of pea (Pisum sativum L). Mol Gen Genet 214: 153–157 (1988).

    Google Scholar 

  8. Feinberg AP, Vogelstein B: A technique for radiolabelling DNA restriction fragments to a high specific activity. Anal Biochem 132: 6–13 (1984).

    Google Scholar 

  9. Ferreira PCG, Hemerly AS, VanMontagu M, Inze D: A protein phosphatase 1 from Arabidopsis thaliana restores temperature sensitivity of a Schizosaccharomyces pombe cdc25ts/wee 1- double mutant. Plant J 4: 81–87 (1993).

    Article  PubMed  Google Scholar 

  10. Hall TC, Ma Y, Buchbinder BU, Pyne JW, Sun SM, Bliss FA: Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterisation. Proc Natl Acad Sci USA 75: 3196–3200 (1978).

    Google Scholar 

  11. Hemmings BA, Adams-Pearson C, Maurer F, Muller P, Goris J, Merlevede W, Hofsteenge J, Stone SR: α- and β-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure. Biochemistry 29: 3166–3173 (1990).

    PubMed  Google Scholar 

  12. Hendrix P, Turowski P, Mayer-Jaekel RE, Goris J, Hofsteenge J, Merlevede W, Hemmings BA: Analysis of subunit isoforms in protein phosphatase 2A holoenzymes from rabbit and Xenopus. J Biol Chem 268: 7330–7337 (1993).

    PubMed  Google Scholar 

  13. Hendrix P, Mayer-Jaekel RE, Cron P, Goris J, Hofsteenge J, Merlevede W, Hemmings BA: Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. J Biol Chem 268: 15267–15276 (1993).

    PubMed  Google Scholar 

  14. Huber SC, Huber JL: Role of sucrose-phosphate synthase in sucrose metabolism in leaves. Plant Physiol 99: 1275–1278 (1992).

    Google Scholar 

  15. Kamibayashi C, Lickteid RL, Estes R, Walter G, Mumby MC: Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits. J Biol Chem 267: 21864–21872 (1992).

    PubMed  Google Scholar 

  16. Kandasamy MK, Thorsness MK, Rundle SJ, Goldberg MI, Nasrallah JB, Nasrallah ME: Ablation of papillar cell function in Brassica flowers results in the loss of stigma receptivity to pollination. Plant Cell 5: 263–275 (1993).

    Article  PubMed  Google Scholar 

  17. Kauss H, Jeblick W: Induced Ca2+ uptake and callose synthesis in suspension-cultured cells of Catharanthus roseus are decreased by the protein phosphatase inhibitor okadaic acid. Physiol Plant 81: 309–312 (1991).

    Article  Google Scholar 

  18. MacKintosh C, Cohen P: Identification of high levels of type 1 and type 2A protein phosphatases in higher plants. Biochem J 262: 335–339 (1989).

    PubMed  Google Scholar 

  19. MacKintosh RW, Haycox G, Hardie DG, Cohen PTW: Identification by molecular cloning of two cDNA sequences from the plant Brassica napus which are very similar to mammalian protein phosphatases-1 and-2A. FEBS Lett 276: 156–160 (1990).

    Article  PubMed  Google Scholar 

  20. MacKintosh C, Coggins J, Cohen P: Plant protein phosphatases: subcellular distribution, detection of protein phosphatase 2C and identification of protein phosphatase 2A as the major quinate dehydrogenase phosphatase. Biochem J 273: 733–738 (1991).

    PubMed  Google Scholar 

  21. Mayer-Jaekel RE, Baumgartner S, Bilbe G, Ohkura H, Glover DM, Hemmings BA: Molecular cloning and developmental expression of the catalytic and 65-kDa regulatory subunits of protein phosphatase 2A in Drosophila. Mol Biol Cell 3: 287–298 (1992).

    PubMed  Google Scholar 

  22. Nitschke K, Fleig U, Schell J, Palme K: Complementation of the cs dis2–11 cell cycle mutant of Schizosaccharomyces pombe by a protein phosphatase from Arabidopsis thaliana. EMBO J 11: 1327–1333 (1992).

    PubMed  Google Scholar 

  23. Pearson WR, Lipman DJ: Improved tools for biological sequence analysis. Proc Natl Acad Sci USA 85: 2444–2448 (1988).

    PubMed  Google Scholar 

  24. Pirck M, Pay A, Heberle-Bors E, Hirt H: Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa. Mol Gen Genet 240: 126–131 (1993).

    Article  PubMed  Google Scholar 

  25. Raz V, Fluhr R: Ethylene signal is transduced via protein phosphorylation events in plants. Plant Cell 5: 523–530 (1993).

    Article  PubMed  Google Scholar 

  26. Ruediger R, VanWart Hood JE, Mumby M, Walter G: Constant expression and activity of protein phosphatase 2A in synchronized cells. Mol Cell Biol 11: 4282–4285 (1991).

    PubMed  Google Scholar 

  27. Ruediger R, Roeckel D, Fait J, Bergqvist A, Magnusson G, Walter G: Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus. Mol Cell Biol 12: 4872–4882 (1992).

    PubMed  Google Scholar 

  28. Rundle SJ, Nasrallah JB: Molecular characterization of type 1 serine/threonine phosphatases from Brassica oleracea. Plant Mol Biol 20: 367–375 (1992).

    PubMed  Google Scholar 

  29. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  30. Scutt CP, Fordham-Skelton AP, Croy RRD: Okadaic acid causes breakdown of self incompatibility in Brassica oleracea: evidence for the involvement of protein phosphatases in the incompatible response. Sex Plant Reprod 6: 282–285 (1993).

    Article  Google Scholar 

  31. Sheen J: Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J 12: 3497–3505 (1993).

    PubMed  Google Scholar 

  32. Shenolikar S, Nairn AC: Protein Phosphatases: Recent Progress, pp. 1–121. Advances Second Messenger Phosphoprotein Research, vol. 23. Raven Press, Ltd., New York (1991).

    Google Scholar 

  33. Siegl G, MacKintosh C, Stitt M: Sucrose-phosphate synthase is dephosphorylated by protein phosphatase 2A in spinach leaves: evidence from the effects of okadaic acid and microcystin. FEBS Lett 270: 198–202 (1990).

    Article  PubMed  Google Scholar 

  34. Smith RD, Walker JC: Isolation and expression of a maize type 1 protein phosphatase. Plant Physiol 97: 677–683 (1991).

    Google Scholar 

  35. Smith RD, Walker JC: Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana. Plant Mol Biol 21: 307–316 (1993).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Plant Molecular Biology Group, Department of Biological Sciences, University of Durham, South Road, DH1 3LE, Durham, UK

    I. Marta Evans, Tony Fawcett & Donald Boulter

  2. Department of Biology, Colorado State University, 80523, Fort Collins, Colorado, USA

    Anthony P. Fordham-Skelton

Authors
  1. I. Marta Evans
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  2. Tony Fawcett
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  3. Donald Boulter
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  4. Anthony P. Fordham-Skelton
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Evans, I.M., Fawcett, T., Boulter, D. et al. A homologue of the 65 kDa regulatory subunit of protein phosphatase 2A in early pea (Pisum sativum L.) embryos. Plant Mol Biol 24, 689–695 (1994). https://doi.org/10.1007/BF00023566

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  • DOI: https://doi.org/10.1007/BF00023566

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