Abstract
A genomic clone of the S11 allele from the self-incompatibility locus (S locus) in Solanum chacoense Bitt. has been isolated by cross-hybridization to the S. chacoense S13 allele and sequenced. The sequence of the S11 allele contains all the features expected for S genes of the Solanaceae, and S11 expression, as assessed by northern blots and RNA-PCR, was similar to that of other S. chacoense S alleles. The S11 protein sequence shares 95% identity with the phenotypically distinct S13 protein of S. chacoense and is the gametophytic S allele with the highest similarity to an existing allele so far discovered. Only 10 amino acid changes differentiate the mature proteins from these two alleles, which sets a new lower limit to the number of changes that can produce an altered S allele specificity. The amino acid substitutions are not clustered, suggesting that an accumulation of random point mutations can generate S allele diversity. The S11 intron is unusual in that it could be translated in frame with the coding sequence, thus suggesting an additional mechanism for the generation of new S alleles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ai Y, Singh A, Coleman CE, Ioerger TR, Kheyr-Pour A, Kao T-H: Self-incompatibility in Petunia inflata: isolation and characterization of cDNAs encoding three S allele-associated proteins. Sex Plant Reprod 3: 130–138 (1990).
Ai Y, Tsai H-S, Kao T-H: Cloning and sequencing of cDNAs encoding two S proteins of a self-incompatible cultivar of Petunia hybrida. Plant Mol Biol 19: 523–528 (1992).
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Current Protocols in Molecular Biology. Wiley, New York (1987).
Bernatzky R, Anderson MA, Clarke AE: Molecular genetics of Self-incompatibility in flowering plants. Devel Genet 9: 1–12 (1988).
Breathnach R, Chambon P: Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem 50: 349–383 (1981).
Clark KR, Okuley JJ, Collins PD, Sims TL: Sequence variability and developmental expression of S alleles in self-incompatible and pseudo-self-compatible petunia. Plant Cell 2: 815–826 (1990).
Clark AG, Kao T-H: Excess nonsynonymous substitution at shared polymorphic sites among self-incompatibility alleles of Solanaceae. proc Natl Acad Sci USA 88: 9823–9827 (1991).
Coleman EC, Kao TH: The flanking regions of two Petunia inflata S alleles are heterogeneous and contain repetitive sequences. Plant Mol Biol 18: 725–737 (1992).
Dayhoff MO, Schwartz RM, Orcutt BC: A model of evolutionary change in proteins. In: Dayhoff M (ed) Atlas of Protein Sequence and Structure, vol 5, Suppl 3, pp. 345–352. National Biomedical Research Foundation, Washington DC (1979).
Després C, Saba El Leil MK, Rivard SR, Morse D, Cappadocia M: Molecular cloning of two Solanum chacoense S alleles and a hypothesis concerning their evolution. Sex Plant Reprod (in press).
Ebert PR, Anderson MA, Bernatzky R, Altschuler M, Clarke AE: Genetic polymorphism of self-incompatibility in flowering plants. Cell 56: 255–262 (1989).
Goodall GJ, Filipovicz W: The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing. Cell 58: 473–483 (1989).
Ioerger TR, Gohlke JR, Xu B, Kao T-H: Primary sequence features of the self-incompatible protein in solanaceae. Sex Plant Reprod 4: 81–87 (1991).
Kaufmann H, Salamini F, Thompson RD: Sequence variability and gene structure at the self-incompatibility locus of Solanum tuberosum. Mol Gen Genet 226: 457–466 (1991).
Kawasaki ES: Amplification of RNA. In: PCR Protocols: A Guide to Methods and Applications. Academic Press, New York (1990).
Kheyr-Pour A, Bintrim SB, Ioerger TR, Remy R, Hammond SA, Kao T-H: Sequence diversity of pistil S proteins associated with gametophytic self-incompatibility in Nicotiana alata. Sex Plant Reprod 3: 88–97 (1990).
Lang BF, Burger G: A rapid, high resolution DNA sequencing gel system. Anal Biochem 188: 176–180 (1990).
McClure BA, Gray JE, Anderson MA, Clarke AE: Self-incompatibility in Nicotiana alata involves degradation of pollen rRNA. Nature 347: 757–760 (1990).
Nasrallah JB, Nishio T, Nasrallah ME: The self-incompatibility genes of Brassica: expression and use in genetic ablation of floral tissues. Annu Rev Plant Physiol Plant Mol Biol 42: 393–422 (1991).
deNettancourt D, Ecochard R, Perquin MDG, van derDrift T, Westerhof M: The generation of new S alleles at the incompatibility locus of Lycopersicon peruvianum Mill. Theor Appl Genet 41: 120–129 (1971).
deNettancourt D: Incompatibility in angiosperms. In: Frankel R, Gall GAE, Linskens HF (eds) Monographs on Theoretical and Applied Genetics, No 3, pp. 1–230. Springer-Verlag, Berlin (1977).
Rivard SR, Cappadocia M, Vincent G, Brisson N, Landry BS: Restriction fragment length polymorphism (RFLP) analyses of plants produced by in vitro anther culture of Solanum chacoense Bitt. Theor Appl Genet 78: 49–56 (1989).
Saba El Leil M, Rivard SR, Morse D, Cappadocia M: A polymorphic molecular marker cosegregates with the S alleles in self-incompatible Solanum chacoense Bitt. Incompatibil Newsl 25: 26–30 (1993).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Singh A, Kao T-H: Gametophytic self-incompatibility: biochemical, molecular genetic, and evolutionary aspects. In: Russell SD, Dumas C (eds) Sexual Reproduction in Flowering Plants. Int Rev Cytol 140: 449–483 (1992).
Sree Ramulu K: Genetic instability at the S locus of Lycopersicon peruvianum plants regenerated from in vitro culture of anthers: generation of new S specificities and S allele reversions. Heredity 49: 319–330 (1982).
Tantikanjana T, Nasrallah ME, Stein JC, Chen C-H, Nasrallah JB: An alternative transcript of the S locus glycoprotein gene in a class II pollen-recessive self-incompatibility haplotype of Brassica oleracea encodes a membrane-anchored protein. Plant Cell 5: 657–666 (1993).
Tsai D-S, Lee H-S, Post LC, Kreiling KM, Kao T-K: Sequence of an S-protein of Lycopersicon peruvianum and comparison with other solanaceous S proteins. Sex Plant Reprod 5: 256–263 (1992).
Twell D, Yamaguchi J, Wing RA, Ushiba J, McCormick S: Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements. Genes Devel 5: 496–507 (1991).
Wright S: The distribution of self-sterility alleles in populations. Genetics 24: 538–552 (1939).
Xu B, Mu J, Nevins DL, Grun P, Kao T-K: Cloning and sequencing of cDNAs encoding two self-incompatibility associated proteins in Solanum chacoense. Mol Gen Genet 224: 341–346 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saba-El-Leil, M.K., Rivard, S., Morse, D. et al. The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar. Plant Mol Biol 24, 571–583 (1994). https://doi.org/10.1007/BF00023555
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00023555