Abstract
The small subunit ribosomal DNA for a woody actinorhizal, Alnus glutinosa, was isolated by the PCR method. Amplification products were cloned into the Bluescript SK- vector. Full sequence, 1698 bp, was obtained with NS1 to NS8 primers. Sequence alignments were made by UWGCG sequence data analysis computer programs. 18S rDNA sequence of A. glutinosa was compared to analogous segments of four other angiosperms, tomato, rice, maize and soybean. Sequence homologies are discussed and application for the technique is suggested.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bousquet J, Simon L, Lalonde M: DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction. Can J For 20: 254–257 (1990).
Bousquet J, Girouard E, Strobeck C, Dancik BP, Lalonde M: Restriction fragment polymorphisms in the rDNA region among seven species of Alnus and Betula papyrifera. Plant Soil 118: 231–240 (1989).
Cesarone CF, Bolognesi C, Santi L. Improved micro-fluorometric DNA determination in biological material using 33258Hoechst. Anal Biochem 100: 188–197 (1979).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Eckenrode VK, Arnold J, Meagher RB: Comparison of the nucleotide sequence of soybean 18S rRNA with the sequence of other small subunit rRNAs. J Mol Evol 21: 259–269 (1985).
Kiss T, Szkukalek A, Solymosy F: Nucleotide sequence of 17S (18S) rRNA gene from tomato: Nucl Acids Re 17: 2127 (1989).
Innis MA, Gelfang DH, Sninsky JJ, White TJ: PCR protocols, a guide to methods and application: Academic Press, San Diego (1990).
Lalonde M, Simon L, Bousquet J, Séguin A: Advances in the taxonomy of Frankia: Recognition of species alni and elaeagni and novel subspecies pommerii and vanddijkii. In: Bothe, de Bruijn, Newton (eds) Nitrogen Fixation: Hundred Years After. Gustav Fisher, New York (1988).
Maniatis TE, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Messing J, Carlson J, Hagen G, Rubenstein I, Oleson A: Cloning and sequencing of the ribosomal RNA genes in maize: The 17S region: DNA 3: 31–40 (1984).
Nairn CJ, Ferl RJ: The complete nucleotide sequence of the small-subunit ribosomal RNA coding region for the cycad Zamia pumila: phylogenetic implications. J Mol Evol 27: 133–141 (1988).
Schmidt-Puchta W, Kütemeier G, Günther I, Haas B, Sänger HL: Cloning and sequence analysis of the 18S ribosomal RNA gene of tomato and secondary structure model for the 18S rRNA of angiosperms. Mol Gen Genet 219: 17–25 (1989).
Takaiwa F, Oono K, Sugiura M: The complete nucleotide sequence of a rice 17S rRNA gene. Nucl Acids Res 12: 5441–5448 (1984).
Tremblay F, Lalonde M: Requirements for in vitro propagation of seven nitrogen-fixing Alnus species: Plant Cell Tissue Organ Culture 189–199 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Savard, L., Lalonde, M. Sequence of 18S rDNA of actinorhizal Alnus glutinosa (Betulaceae). Plant Mol Biol 16, 725–728 (1991). https://doi.org/10.1007/BF00023436
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00023436