Summary
Eight triazine resistant (Brassica napus x B. oleracea) ♀ x B. oleracea ♂ interspecific hybrids with chromosome numbers ranging from 25 to 27 were backcrossed a second time to B. oleracea but no seed was formed. However, in vitro embryo rescue on 77 developing ovules yielded nine BC2 plants with chromosome numbers between 19 and 25 and in which the herbicide resistance was still strongly expressed. Three of these plants (NOH-8B2B1, 2n=20; NOH-8B2B3 and NOH-8B2B4, 2n=19) were backcrossed again to B. oleracea. Two of the three plants produced seed which germinated to produce triazine resistan BBC3s with 18, 19 or 20 chromosomes. The triazine resistant B. campestris cytoplasm has now been stabilized in B. oleracea.
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Ayotte, R., Harney, P.M. & Machado, V.S. The transfer of triazine resistance from Brassica napus L. to B. oleracea L. IV. Second and third backcrosses to B. oleracea and recovery of an 18-chromosome, triazine resistant BC3 . Euphytica 40, 15–19 (1989). https://doi.org/10.1007/BF00023292
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DOI: https://doi.org/10.1007/BF00023292