Summary
In the genus Petunia, forty inbred, lines and related species, were compared for their regeneration capacity at the seedling stage. Individual seedlings were observed as positive or negative for the presence of neoformed buds at five locations; it was observed that the presence of buds in the axillary zone of cotyledons, around their insertions and up their surface were the best estimators of differences in regeneration capacity. A very large scale of variation was found between these lines and species. After this first experiment, genotypes were selected for a further study: responses to four cytokinin concentrations were evaluated. Genotypic effects, cytokinin effects and genotype-cytokinin interactions were highly significant. Five F1 hybrids between High or Low responsive lines and the same tester were compared with their parental forms under this basis. The major genetic component was additive; a maternal effect was frequently found between reciprocal hybrids. The cytokinin effects and genotype-cytokinin interactions were highly significant. A single couple of parents and their hybrids were choosen for a backcross generation. The segregation ratio observed between cloned individuals from the backcross suggests that two loci should be responsible for the differences observed between these parental forms.
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Dulieu, H. Inheritance of the regeneration capacity in the genus Petunia . Euphytica 53, 173–181 (1991). https://doi.org/10.1007/BF00023269
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DOI: https://doi.org/10.1007/BF00023269