, Volume 67, Issue 1–2, pp 95–99 | Cite as

Identification of soybean strains resistant to Xanthomonas campestris pv. glycines

  • Arun Sharma
  • P. M. Nair
  • S. E. Pawar


Soybean germplasm was screened for resistance to bacterial pustule disease. The etiological agent, Xanthomonas campestris pv. glycines, was isolated from the leaves of field grown soybean in Maharashtra, India. The screening of soybean stocks was carried out by excised leaf inoculation method. A differential susceptibility to the pathogen was observed in the tested stocks. Two stocks P-4-2 and P-169-3 were found to be completely resistant to the pathogen and displayed an incompatible reaction. Four cultivars, EC-34160, Bragg, Kalitur and PK-472 displayed moderate resistance and the remaining stocks were susceptible to the attack of the pathogen. The stocks P-4-2 and P-169-3 remained resistant even to a high concentration of 109 colony forming units (cfu)/ml of the pathogen.

Key words

Glycine max. soybean Xanthomonas campestris pv. glycines bacterial pustule disease variation resistance 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chamberlain, D.W., 1962. Reaction of resistant and susceptible soybeans to Xanthomonas phaseoli var. sojensis. Plant Disease Rep., 46: 707–709.Google Scholar
  2. Fett, W.F., 1984. Accumulation of isoflavonoids glycosides after inoculation of soybean leaves and a study of their role in resistance. Physiol. Plant Pathol., 24: 303–320.Google Scholar
  3. Groth, D.E. & E.J.Braun, 1986. Growth kinetics and histopathology of Xanthomonas campestris pv. glycines in leaves of resistant and susceptible soybeans. Phytopathol. 76: 959–966.Google Scholar
  4. Hartwig, E.E. & S.G.Lehman, 1951. Inheritance of resistance to bacterial pustule disease in soybeans. Agronomy J., 43: 226–229.Google Scholar
  5. Kearny, B. & B.Staskawicz, 1990. Widespred distribution and fitness contribution of Xanthomonas campestris avirulence gene avrBs2. Nature, 346: 385–386.Google Scholar
  6. Keen, N.T., 1990. Gene-for-gene complementarity in plant pathogen interactions. Ann. Rev. Genet., 24: 447–463.Google Scholar
  7. Keen, N.T., D.Koayashi, S.Tamaki, H.Shen, M.Stayton, D.Lawrence, A.Sharma, S.Midland, M.Smith & J.Sims, 1991. Avirulence gene D from Pseudomonas syringae pv. tomato and its interaction with resistance gene Rpg4 in soybean. In: H.Henneck & D.P.S.Verma (Eds.) Advances in molecular genetics of plant-microbe interaction, Vol. 1, 37–44, Kluwer Academic Publishers, The Netherlands.Google Scholar
  8. Nene, Y.L., 1988. Multiple disease resistance in grain legumes. Ann. Rev. Phytopathol. 26: 203–217.Google Scholar
  9. Parthuangwong, S. & K.Amnuaykit, 1987. Studies on tolerance and rate reducing bacterial pustule of soybean cultivars/lines. Kesetsart J. of Nat. Sci. 21: 408–426.Google Scholar
  10. Reddy, K.S., S.E.Pawar & C.R.Bhatia, 1987. Screening for powdry mildew (Erysiphe polygoni DC) resistance in mungbean (Vigna radiata (L.) Wilczek) using excised leaves. Proc. Indian Acad. Sci. (Plant Sci.), 97: 365–369.Google Scholar
  11. Verma, V.D., 1990. Evaluation of soybean (Glycine max) germplasm for bacterial pustules caused by Xanthomonas campestris pv. glycines. Ind. J. Agric. Sci., 60: 832–833.Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Arun Sharma
    • 1
  • P. M. Nair
    • 1
  • S. E. Pawar
    • 2
  1. 1.Food Technology and enzyme engineering DivisionBhabha Atomic Research CentreBombayIndia
  2. 2.Nuclear Agriculture DivisionBhabha Atomic Research CentreBombayIndia

Personalised recommendations