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Variation of the storage protein phaseolin in common bean (Phaseolus vulgaris L.) from the Mediterranean area

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Summary

Variation of phaseolin, the major storage protein in Phaseolus vulgaris L., was analysed in seeds of a germplasm collection from the Mediterranean area. Results showed a number of subvariants differing from the reference patterns for the presence, absence or faintness of single polypeptides. Maximum concentration of variation was found in the Cyprus material. Sources and causes of variation are discussed.

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References

  • Bliss, F.A. & J.W.S. Brown, 1983. Breeding common bean for improved quantity and quality of seed protein. In: J. Janick (Ed.), Plant Breeding Reviews Vol. 1 p. 59–102. AVI Publ. Co., Westport, Connecticut.

    Google Scholar 

  • Brown, J.W.S., Y. MA, F.A. Bliss & T.C. Hall, 1981. Genetic variation in the subunits of globulin-1 storage protein of french bean. Theor. Appl. Genet. 59: 83–88.

    Google Scholar 

  • Brown, J.W.S., J.R. McFerson, F.A. Bliss & T.C. Hall, 1982. Genetic divergence among commercial classes of Phaseolus vulgaris in relation to phaseolin pattern. HortScience 17: 752–754.

    Google Scholar 

  • Evans, A.M., 1980. Structure, variation, evolution, and classification in Phaseolus. In: R.J. Summerfield & A.H. Bunting (Eds), Advances in Legume Science p. 337–347. HMSO Publ., London.

    Google Scholar 

  • Hall, T.C., R.C. McLeester & F.A. Bliss, 1977. Equal expression of the maternal and paternal alleles for the polypeptide subunits of the major storage protein of the bean Phaseolus vulgaris L. Plant Physiol. 59: 1122–1124.

    Google Scholar 

  • Gepts, P., T.C. Osborn, K. Rashka & F.A. Bliss, 1986. Phaseolin-protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple centers of domestication. Econ. Bot. 40: 451–468.

    Google Scholar 

  • Gepts, P. & F.A. Bliss, 1988. Dissemination pathways of common bean (Phaseolus vulgaris, Fabaceae) deduced from phaseolin electrophoretic variability. II. Europe and Africa. Econ. Bot. 42: 86–104.

    Google Scholar 

  • Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    PubMed  Google Scholar 

  • Lafiandra, D. & D.D. Kasarda, 1985. One-and two-dimensional (two-pH) polyacrylamide gel electrophoresis in a single gel: separation of wheat proteins. Cereal Chem. 62: 314–319.

    Google Scholar 

  • Lioi, L. & R. Bollini, 1984. Contribution of processing events to the molecular heterogeneity of four banding types of phaseolin, the major storage protein of Phaseolus vulgaris. Plant Mol. Biol. 3: 345–353.

    Google Scholar 

  • Lioi, L., 1989. Geographical variation of phaseolin patterns in an old world collection of Phaseolus vulgaris. Seed Sci. Technol. 17.

  • Matta, N.K., J.A. Gatehouse & D. Boulter, 1981. Molecular and subunit heterogeneity of legumin of Pisum sativum L. (garden pea) — a multi-dimensional gel electrophoretic study. J. Exp. Bot. 32: 1295–1307.

    Google Scholar 

  • Muntz, K., C. Horstmann & B. Schlesier, 1986. Seed proteins and their genetics in Vicia faba L. Biol. Zentralbl. 105: 107–120.

    Google Scholar 

  • O'Farrell, P.H., 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007–4021.

    PubMed  Google Scholar 

  • Slightom, J.L., R.F. Drong, R.C. Klassy & L.M. Hoffman, 1985. Nucleotide sequences for phaseolin cDNA clones: the major storage proteins from Phaseolus vulgaris are encoded by two unique gene families. Nucl. Acids Res. 13: 6483–6497.

    PubMed  Google Scholar 

  • Staswick, P.E. & N.C. Nielsen, 1983. Characterization of a soybean cultivar lacking certain glycinin subunits. Arch. Biochem. Biophys. 223: 1–8.

    PubMed  Google Scholar 

  • Talbot, D.R., M.J. Adang, J.L. Slightom & T.C. Hall, 1984. Size and organization of a multigene family encoding phaseolin, the major storage protein of Phaseolus vulgaris L. Mol. Gen. Genet. 198: 42–49.

    Article  Google Scholar 

  • Vavilov, N.I., 1951. Phytogeographic basis of plant breeding. In: F. Verdoorn (Ed.), The origin, variation, immunity and breeding of cultivated plants p. 13–54. The Ronald Press Co., New York.

    Google Scholar 

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  1. Germplasm Institute, CNR, Via Amendola 165/A, 70126, Bari, Italy

    Lucia Lioi

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  1. Lucia Lioi
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Lioi, L. Variation of the storage protein phaseolin in common bean (Phaseolus vulgaris L.) from the Mediterranean area. Euphytica 44, 151–155 (1989). https://doi.org/10.1007/BF00022610

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  • DOI: https://doi.org/10.1007/BF00022610

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