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Production of non-chimeral colchiploids in Rubus species by tissue culture

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Summary

Diploid Rubus allegheniensis Porter and R. rusticanus Merc. shoot tips were proliferated in tissue culture and treated with 0.02 to 0.08% colchicine to induce non-chimeral polyploidy. About 20 shoot tips of each treatment were transferred to test tubes containing modified Anderson's medium for shoot proliferation. Shoots at the 3- or 4-leaf stage were transplanted into Jiffy-7 peat pellets and placed under intermittent mist for rooting. Root tip squashes were made for chromosome counts. Thirteen R. allegheniensis and 7 R. rusticanus plants proved to be autotetraploids (28 chromosomes). Stomata of the autotetraploids were significantly larger than those of the diploids but stomatal conductance of CO2 and photosynthesis rate were reduced significantly as compared to diploids. Pollen grains in tetraploid were larger than those in diploid R. allegheniensis plants. These results indicate that tissue culture can facilitate the production of non-chimeral colchiploids in Rubus spp.

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Authors and Affiliations

  1. Small Fruit Research Station, USDA, ARS, 39470, Poplarville, MS, USA

    C. L. Gupton

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  1. C. L. Gupton
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Gupton, C.L. Production of non-chimeral colchiploids in Rubus species by tissue culture. Euphytica 44, 133–135 (1989). https://doi.org/10.1007/BF00022607

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  • DOI: https://doi.org/10.1007/BF00022607

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