Summary
Embryo development in vivo was compared in the two barley genotypes VK 16032 and ‘Vogelsanger Gold’ after self pollination and after pollination with H. bulbosum. Embryo growth in VK 16032 after crossing with H. bulbosum showed a similar growth pattern to that of the self pollinated embryos, although the size increase was smaller. The hybrid embryos continued to grow until day 18 after pollination. The embryos from the cross between ‘Vogelsanger Gold’ and H. bulbosum ceased to grow 8 days after pollination. Arrested embryo growth with subsequent abortion in ‘Vogelsanger Gold’ was associated with a very early depletion and break down of the endosperm. Use of barley genotypes less sensitive to the genomic disturbances after crossing with H. bulbosum in combination with genotypes like ‘Vogelsanger Gold’ is recommended in haploid breeding work.
Similar content being viewed by others
References
Adamski, T., 1979. The obtaining of autotetraploid barley lines using haploids from the cross Hordeum vulgare L. x Hordeum bulbosum L. Genetica Polonica 20: 31–42.
Bennett, M.D., R.A. Finch & I.R. Barclay, 1976. The time rate and mechanism of chromosome elimination in Hordeum hybrids. Cromosoma 54: 175–200.
Björnstad, Å, 1986. Partial incompatibility between Scandinavian six-rowed barleys (Hordeum vulgare L.) and Hordeum bulbosum L., and its genetical basis. Hreditas 104: 171–191.
Chen, F. & P. Hayes, 1991. Effect of exogenous plant growth regulators on in vitro seed growth, embryo development, and haploid production in a cross between H. vulgare (L.) and H. bulbosum (L.). Plant Cell Tissue and Organ Culture 25: 179–184.
Bukuyama, T. & H. Hosoya, 1983. Genetic control and mechanism of chromosome elimination in the hybrids between Hordeum bulbosum (4x) and H. vulgare (4x). Jpn. J. Genet. 58: 241–250.
Hanzel, J.J., M.A. Miller, E. Brinkmann & E. Fendos, 1985. Genotype and media effects on callus formation and regeneration in barley. Crop Sci. 25: 27–31.
Hayes, P.M. & F.Q. Chen, 1989. Genotypic variation for Hordeum bulbosum L.-mediated haploid production in winter and facultative barley. Crop Sci. 29: 1184–1188.
Ho, K.M. & K.J. Kasha, 1975. Genetic control of chromosome elimination during haploid formation in barley. Genetics 81: 263–275.
Jensen, C.J., 1975. Barley monoploids and doubled monoploids: techniques and experience. In: Barley Genetics III, Proc. IIIrd Int. Barley Genetic Symp., pp. 316–345. Verlag Karl Thiemig, München.
Kasha, K.J., N.C. Subrahmanyam & A. Ali, 1978. Effect of gibberellic acid treatment, and nutrient supply through detached tillers, upon haploid frequency in barley. Theor. Appl. Genet. 51: 169–175.
Kunert, R. & H. Peterka, 1984. Effectiveness of embryo culture in haploid barley production in relation to embryo differentiation and size. In: F.J. Novak, L. Havel & J. Dolezel (Eds.), Plant tissue and cell culture application to crop improvement, pp. 255–256. Czechoslovak Academy of Sciences, Prague.
Larkin, P.J. & W.R. Scowcroft, 1981. Somaclonal variation—a novel source of variability from cell culture for plant improvement. Theor. Appl. Genet. 60: 197–214.
Lührs, R. & H. Lörz, 1986. Somatic embryogenesis, cell and protoplast culture of Hordeum vulgare L. (barley). In: W. Horn, C.J. Jensen, W. Odenbach & O. Schieder (Eds.), Genetic Manipulation in Plant Breeding, pp. 483–486. Walter de Gruyter, Berlin.
Pickering, R.A. & W.F. Rennie, 1990. The evaluation of superior Hordeum bulbosum L. genotypes for use in a doubled haploid barley breeding programme. Euphytica 45: 251–255.
Scowcroft, W.R., 1985. Somaclonal variation: the myth of clonal uniformity. In: B. Hohn & E.S. Dennis (Eds.), Genetic Flux in Plants, pp. 217–245. Vienna, Springer Verlag.
Simpson, E., J.W. Snape & R.A. Finch, 1980. Variation between Hordeum bulbosum genotypes in their ability to produce haploids of barley, Hordeum vulgare. Z. Pflanzenzücht. 85: 205–211.
Subrahmanyam, N.C. & K.J. Kasha, 1973. Selective chromosomal elimination during haploid formation in barley following interspecific hybridization. Chromosoma 42: 111–125.
Thomas, H.M. & R.A. Pickering, 1983. Chromosome elimination in Hordeum vulgare x H. bulbosum hybrids I. Comparison of stable and unstable amphidiploids. Theor. Appl. Genet. 66: 135–140.
Thörn, E.C., 1991. Crossability barriers between barley (Hordeum vulgare L.) and H. bulbosum L. Genetic studies of the partial incompatibility mechanisms in the barley cultivars ‘Vada’ and ‘Vogelsanger Gold’. Hereditas 114: 213–218.
Thörn, E.C., 1992a. The influence of genotype and environment on seed and embryo development in barley (Hordeum vulgare L.) after crossing with Hordeum bulbosum L. Euphytica 59: 109–118.
Thörn, E.C. 1992b. Plant regeneration from embryos derived from crosses between barley (Hordeum vulgare L.) and Hordeum bulbosum L. Hereditas (in press).
Xu, J. & J.W. Snape, 1988. The cytology of hybrids between Hordeum vulgare and H. bulbosum revisited. Genome 30: 486–494.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thörn, E.C. Embryo development in two barley genotypes after self pollination and pollination with Hordeum bulbosum L.. Euphytica 65, 93–98 (1992). https://doi.org/10.1007/BF00022570
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00022570