Summary
A method is described for selecting resistant transformed tomato genotypes in vitro at the stage of immature embryos. The utilization of HLH nutrient medium with the selective agent kanamycin is proposed. Normal development of seedlings from immature embryos, which do not form callus, is a good and true indicator for isolation of resistant genotypes. The immature embryos do not germinate and develop on MS selective medium.
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Chesnokov, Y.V., Sedov, G.I. In vitro selection of tomato transformants at the immature embryo stage. Euphytica 81, 79–83 (1995). https://doi.org/10.1007/BF00022461
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DOI: https://doi.org/10.1007/BF00022461