Segregation of plant height (PH), tiller number (TN), panicle number (PN), average panicle length per plant (PL), average primary branch number per panicle per plant (PBN) and 1000 grain weight (1000G) were specific in an F2 population derived from a cross of Palawan, a tall Javanica variety, and IR42, an Indica semidwarf variety. One hundred and four informative RFLP markers covering all 12 chromosomes were used for detecting putative QTLs controlling the traits. Orthogonal contrasts and interval mapping analysis were used for the analysis. QTL detected for PH on the region of chromosome 1, where semidwarfing gene sd-1 locus is located, seems to be a multiple allelic locus. An additional QTL for PH was identified on chromosome 2. Two QTLs for TN were detected on chromosomes 4 and 12. The QTL on chromosome 4 seemed also to govern the variation in PN. Four QTLs were found for the other traits, two of them for PL were located on chromosomes 6 and 2, one for PBN on chromosome 6 and the other for 1000G on chromosome 1. Additive gene actions were found to be predominant, except one QTL for PH and one QTL for PL, but partial or incomplete dominance also existed for the QTLs detected.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
Beavis, W.D., D. Grant, M. Albertsen & R. Fincher, 1991. Quantitative trait loci for plant height in four maize populations and their associations with quantitative genetic loci. Theor. Appl. Genet. 83: 141–145.
Causse, M., T.M. Fulton, Y.G. Cho, S.N. Ahn, J. Chunwongse, K. Wu, J. Xiao, Z.H. Yu, P.C. Ronald, S.B. Harrington, G.A. Second, S.R. McCouch & S.D. Tanksley, 1994. Saturated molecular map of the rice genome based on an inter-specific backcross population. Genetics 138: 1251–1274.
Champoux, M.C., G. Wang, S. Sarkarung, D.J. Mackill, J.C. O'Tool, N. Huang & S.R. McCouch, 1994. Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor. Appl. Genet. (in press).
Cho, Y.G., 1992. Genetics of esterase isozymes and RFLP markers and their linkage with a semidwarf gene (sd-1) in rice (Oryza sativa L.). Research Reports of the Rural Development Administration, Biotechnology (English abstract) 34: 37–77.
Crow, J.F., 1952. Dominance and overdominance. pp. 282–297. In: J.W. Gowen (Ed). Heterosis. Iowa State College Press, Ames, IA.
Edwards, M.D., C.W. Stuber & J.F. Wendel, 1987. Molecular-marker-facilitated investigation of quantitative trait loci in maize. I. Number, genetic distribution and types of gene action. Genetics 113: 113–125.
Feinberg, A.P. & B. Vogelstein, 1984. A technique for radiolabelling DNA restriction fragments to a high specific activity. Anal. Biochem. 132: 6–13.
IRRI, 1975. Parentage of IRRI crosses IR1-IR50,000, International Rice Research Institute, Manila, Philippines.
Lander, E.S., 1993. Mapmaker/EXP 3.0 and Mapmaker/QTL 1.1, Tutorial and Reference Manual. Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142.
Lander, E.S. & D. Botstein, 1989. Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199.
Ogi, Y., H. Kato, K. Maruyama, A. Saito & F. Kikuchi, 1993. Identification of RFLP markers closely linked to the semidwarfing gene at the sd-1 locus in rice. Jap. J. Breeding 43: 141–146.
Plaschke, J., A. Borner, D.X. Xie, R.M.D. Koebner, R. Schlegel & M.D. Gale, 1993. RFLP mapping of genes affecting plant height and growth habit in rye. Theor. Appl. Genet. 85: 1049–1054.
Soller, M., T. Brody & A. Genizi, 1976. On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor. Appl. Genet. 47: 35–39.
Stuber, C.W., 1989. Marker-based selection for quantitative traits. In: Proc. XII Congr. Eucarpia. Parey, Berlin Hamburg, pp. 31–49.
Stuber, C.W., M.D. Edwards & J.F. Wendel, 1987. Molecular-marker-facilitated investigation of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci. 27: 639–648.
Tsai, K.H., 1986. Possible genetic differences between two Taichung 65 strains, one preserved at Taichung and the other from Ryukyus. RGN 3: 75.
Tsai, K.H., 1991. Multiple alleles detected at locus sd-1. RGN 8: 112–113.
Wang, G.L., D.J. Mackill, J.M. Bonman, S.R. McCouch & R.J. Nelson, 1994. RFLP mapping of genes conferring complete and partial resistance to blast in a durably-resistant rice cultivar. Genetics 136: 1421–1433.
Wu, P., G. Zhang & N. Huang, 1995a. Non-allelic interaction conditioning spicklet sterility in an F2 population of Indica/Javanica cross in rice. Theor. Appl. Genet. 91: 825–829.
Wu, P., G. Zhang, J.K. Ladha, S.R. McCouch & N. Huang, 1995b. Molecular-marker-facilitated investigation on the ability to stimulate N2 fixation in the rhizosphere by irrigated rice plants. Theor. Appl. Genet. (in press).
Wu, Z., 1991. Molecular mapping of rice (Oryza sativa L.) genes via linkage to restriction fragment length polymorphism (RFLP) markers. A dissertation presented to the Faculty of the Graduate School of Cornell University.
About this article
Cite this article
Wu, P., Zhang, G. & Huang, N. Identification of QTLs controlling quantitative characters in rice using RFLP markers. Euphytica 89, 349–354 (1996). https://doi.org/10.1007/BF00022292
- Oryza sativa
- quantitative trait loci
- gene mapping
- restriction fragment
- length polymorphism