Skip to main content
Log in

Analysis of pollen tube growth in situ to investigate self-incompatibility in the wild potato Solanum commersonii

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

Pollen tube growth was investigated in a diallelic crossing design with seven genotypes of the diploid wild potato species Solanum commersonii, accession O/S UR-9, CIP 762459. Pollen tube growth in the style was recorded using a combined quantitative and qualitative evaluation scale. Clear-cut differences in pollen tube growth behavior in compatible and in partially or completely incompatible crosses were detected. Diallelic crossing of the seven randomly chosen genotypes, intercrossing within two progeny families, and backcrossing of two progeny populations to the parents revealed the existence of a one-locus gametophytic system of stylar incompatibility. The S-allele status of all genotypes investigated was determined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abdalla M.M.F., 1970. Inbreeding, heterosis, fertility, plasmon differentiation and Phytophthora resistance in Solanum verrucosum Schlechtd., and some interspecific crosses in Solanum. Agric. Res. Rep. 748, Pudoc, Wageningen, The Netherlands, 213 pp.

    Google Scholar 

  • Abdalla M.M.F. & J.G.Th. Hermsen, 1971. A two-loci system of gametophytic incompatibility in Solanum phureja and S. stenotomum. Euphytica 21: 345–350.

    Google Scholar 

  • Ai Yunjun, A. Singh, C.E. Coleman, T.R. Ioerger, A. Kheyr-pour & T.-H. Kao, 1990. Self-incompatibility in Petunia inflata: isolation and characterization of cDNAs encoding three S-allele-associated proteins. Sex. Plant Reprod. 3: 130–138.

    Google Scholar 

  • Anderson M.A., G.I. McFadden, R. Bernatzky, A. Atkinson, T. Orpin, H. Dedman, G. Tregear, R. Fernley & A.E. Clarke, 1989. Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. The Plant Cell 1: 483–491.

    Google Scholar 

  • Bamberg J.B. & R.E. HannemanJr., 1991. Rapid ploidy screening of tuber-bearing Solanum (potato) species through pollen diameter measurement. Amer. Potato J. 68: 279–285.

    Google Scholar 

  • Broothaerts W.J., P. Vanvinckenroye, B. Decock, J.Van Damme & J.c. Vendrig, 1991. Petunia hybrida S-proteins: ribonuclease activity and the role of their glycan side chains in selfincompatibility. Sex. Plant Reprod. 4: 258–266.

    Google Scholar 

  • Chavez R., P.E. Schmiediche, M.T. Jackson & K.V. Raman, 1988. The breeding potential of wild potato species resistant to the potato tuber moth, Phthorimaea operculella (Zeller). Euphytica 39: 123–132.

    Google Scholar 

  • Chetelat R.T. & J.W. DeVerna, 1991. Expression of unilateral incompatibility in pollen of Lycopersicon pennellii is determined by major loci on chromosomes 1, 6 and 10. Theor. Appl. Genet. 82: 704–712.

    Google Scholar 

  • Cipar M.S., S.J. Peloquin & R.W. Hougas, 1964. Inheritance of incompatibility in hybrids between Solanum tuberosum haploids and diploid species. euphytica 13: 163–172.

    Google Scholar 

  • Clark K.R., J.J. Okuley, P.D. Collins & T.L. Sims, 1990. Sequence variability and developmental expression of S-alleles in selfincompatible and pseudo-self-compatible Petunia. The Plant Cell 2: 815–826.

    Google Scholar 

  • Correll D.S., 1962. The potato and its wild relatives. Texas Research Foundation, Renner, Texas. 606 pp.

    Google Scholar 

  • East E.M. & A.J. Magelsdorf, 1925. A new interpretation of the behavior of self-sterile plants. Proc. Natl. Acad. Sci. USA 11: 166–171.

    Google Scholar 

  • Ehlenfeldt M.K. & R.E. HannemanJr., 1988. The transfer of the synaptic gene (sy-2) from 1EBN Solanum commersonii Dun. to 2EBN germplasm. Euphytica 37: 181–187.

    Google Scholar 

  • Grun P. & M. Aubertin, 1966. The inheritance unilateral incompatibility in Solanum. Heredity 21: 131–138.

    Google Scholar 

  • Hanneman R.E.Jr., 1989. Interspecific crossability studies among 1EBN species. Amer. Potato J. 66: 524.

    Google Scholar 

  • Johnston S.A. & R.E. HaNNemanJr., 1981. The discovery of additional one endosperm balance number (EBN) in diploid Solanum species. Amer. Potato J. 58: 505–506.

    Google Scholar 

  • Kaufmann H., F. Salamini & R.D. Thompson, 1991. Sequence variability and gene structure at the self-incompatibility locus of S. tuberosum. Mol. Gen. Genet. 226: 457–466.

    Google Scholar 

  • Lewis D., 1947. Competition and dominance of incompatibility alleles in diploid pollen. Heredity 1: 85–108.

    Google Scholar 

  • Lewis D. & L.K. Crowe, 1958. Unilateral interspecific incompatibility in flowering plants. Heredity 12: 233–256.

    Google Scholar 

  • McClure B.A., He Du, Ying-Hong Liu & A.E. Clarke, 1993. Slocus products in Nicotiana alata pistils are subject to organspecific post-transcriptional processing but not post-translational processing. Plant Mol. Biol. 22: 177–181.

    Google Scholar 

  • Nasrallah J.B., Takeshi Nishio & M.E. Nasrallah, 1991. The selfincompatibility genes of Brassica: Expression and use in genetic ablation of floral tissues. Ann. Rev. Plant Physiol. Plant. Mol. Biol. 42: 393–422.

    Google Scholar 

  • Novy R.G. & R.E. HannemanJr., 1991. Hybridization between Gp. Tuberosum haploids and 1EBN wild potato species. Amer. Potato J. 68: 151–169.

    Google Scholar 

  • Olsder J. & J.G.TH. Hermsen, 1976. Genetics of self-compatibility in dihaploids of Solanum tuberosum L. I. Breeding behavior of two self-compatible dihaploids. Euphytica 25: 597–607.

    Google Scholar 

  • Pandey K.K., 1962. Interspecific incompatibility in Solanum species. Amer. J. Bot. 49: 874–882.

    Google Scholar 

  • Saba-El-Leil M.K., S. Rivard, D. Morse & M. Cappadocia, 1994. The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar. Plant Mol. Biol. 24: 571–583.

    Google Scholar 

  • Schreiter J. & H. Tiemann, 1977. Die Prüfung des Pollenschlauchwachstums in vivo bei Dihaploiden von Solanum tuberosum L. Archiv f. Züchtungsforschung 7: 253–258.

    Google Scholar 

  • Trognitz, B.R., 1990. Untersuchungen über die Blüh-und Befruchtungsverhältnisse und die Samenentwicklung bei Dihaploiden der Kulturkartoffel. Ph. D. Thesis, Institut f. Kartoffelforschung, Groβ Lüsewitz, Germany. 107 pp.

  • Trognitz B.R., 1991. Comparison of different pollen viability assays to evaluate pollen fertility of potato. Euphytica 56: 143–148.

    Google Scholar 

  • Trognitz B.R. & P.E. Schmiediche, 1993. A new look at incompatibility relationships in higher plants. Sex. Plant Reprod. 6: 183–190.

    Google Scholar 

  • Xu B., P. Grun, A. Kheyr-Pour & T.-H. Kao, 1990. Identification of pistil-specific proteins associated with three self-incompatibility alleles in Solanum chacoense. Sex. Plant Reprod. 3: 54–60.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Trognitz, B.R. Analysis of pollen tube growth in situ to investigate self-incompatibility in the wild potato Solanum commersonii . Euphytica 86, 149–156 (1995). https://doi.org/10.1007/BF00022021

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00022021

Key words

Navigation