Summary
Dry seeds of palmarosa (Cymbopogon martinii Stapf) var. motia were subjected to 12 doses of gamma rays ranging from 5 kr to 100 kr to find out the effects of gamma irradiation on growth parameters, herbage yield and yield and quality of essential oil. Several biological parameters such as seedling growth, pollen fertility, mitotic chromosome behaviour, productive tillers/plant, leaf area index, leaf/stem ratio (by fresh weight) and total herbage yield/ /plant together with yield and quality of oil have been studied in the first (M1) generation. Marked stimulatory effects of gamma radiation in 10 kr and 15 kr doses have been compared with the control for herbage yield, oil percentage and total alcohols (free geranyl and geranyl acetate).
The two yield contributing traits, herbage yield/plant and leaf area index were remarkably increased in 15 kr, which indicates that in palmarosa the 15 kr dose is more effective than the higher doses in producing microlevel mutations. The yield and quality of oil at 10 kr and 15 kr doses were enhanced significantly. The results on high yield and quality of oil as induced by gamma-irradiation were discussed from the point of view that a gene may have been altered by mutagen treatment to produce a metabolic block between geraniol and geranyl acetate on the biosynthetic pathway or that ‘modifier’ genes controlling the expression of a key precursor molecule may have been further modified to give rise to a geraniol rich ‘chemotype’ in palmarosa.
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References
Bremer-Reinders, D. E., 1962. Mutation research in Canary grass. Euphytica 11: 293–310.
Benthrope, D. V., G. N. J., Le, Patoures & M. J. O., Francis, 1972. Biosynthesis of geraniol and nerol and their B-D-glucosides in Pelargonium graveolens and Rosa dilecta. Biochem. J. 130: 1045–54.
Brossman, G. D. & J. R., Wilcox, 1984. Induction of genetic variation for oil properties and agronomic characteristics of soybean. Crop Sci. 24: 783–787.
Busey, P., 1980. Gamma ray dosage and mutation breeding in St. Augustine grass. Crop Sci. 20: 181–184.
Dnyansagar, V. R. & A. R., Pingle, 1977. Correlation between macro mutants and solasodine content in Solanum viarum. Planta Medica 31 (1): 21–25.
Gaul, H. 1970. Sterility. In: Manual on mutation breeding. IAEA, Vienna. pp. 95–99.
Gordon, S. A., 1957. The effects of ionizing radiation on plants: biochemical and physiological aspects. Q. Rev. Biol. 32: 3–14.
Gunckel, J. E. & A. H., Sparrow, 1954: Aberrant growth in plants induced by ionizing radiations. Brookhaven Symp. Biol. 6: 252–279.
Hegnauer, R., 1975. Secondary metabolites and crop plants. In: Frankel, O. H. & J. G., Hawkes (Eds), Crop genetic resources for today and tomorrow. Cambridge University Press, London, p. 249.
Hutton, E. M., 1961. Inter-variety variation in Rhoades grass (Chloris gayana). J. Br. Grassl. Soc. 16: 23–29.
Kaul, B. L. & D. K., Choudhary, 1975. The effect of chemical mutagens on quantitative characters in Atropa belladonna. Planta Media 27 (4): 337–342.
Krishna, G., G., Shivashankar & J., Nath, 1984. Mutagenic response of Rhodes Grass (Chloris gayana Kunth.) to gamma rays. Envir. Exptl. Biol. 24: 197–205.
Levy, A., 1982. Natural and induced genetic variation in the biosynthesis of alkaloids and other secondary metabolites. In: Improvement of oil seed and industrial crops by induced mutations. IAEA, Vienna, pp. 213–222.
Micke, A., 1976. Bibliography. Examples of literature related to the use of induced mutations in cross breeding. In: Induced mutations in cross-breeding. IAEA, Vienna, pp. 233–252.
Mikaelsen, K., 1968. Effect of fast neutrons on seedling growth and metabolism in barley. In: Symp. on neutron irradiation of seeds. II. IAEA, Vienna, pp. 63–70.
Miller, P. D., K. C., Vaughn & K. G., Wilson, 1984. Ethyl methanesulfonate induced chloroplast mutagenesis in crops. Induction and ultrastructure of mutants. Jour. Hered. 75: 86–92.
Powell, J. B., g. W., Burton & J. R., Young, 1974. Mutations induced in vegetatively propagated turf bermudagrass by gamma radiation. Crop Sci. 14: 327–330.
Powell, J. B., 1976. Induced mutations in highly heterozygous vegetatively propagated grasses. In: Induced mutations in cross-breeding. IAEA, Vienna, pp. 290–294.
Powell, J. B. & R. W., Toler, 1980. Induced mutations in ‘Floratam’ St. Augustine grass. Crop Sci. 20: 644–646.
Singh, B. B., 1974. Radiation induced changes in catalase lipase and ascorbic acid of safflower seeds during germination. Radiat. Bot. 14: 195–199.
Srivastava, H. K., 1984. Prospects for genetic engineering in medicinal and aromatic plants. In: Y. S. Murti Commemoration Volume, Meerut University, Trends Pl. Res. 1: 327–341.
Trease, G. E. & W. C., Evans, 1983. Genetics and comparative phytochemistry. In: Pharmacognosy. Baillere Tindal, London, p. 225.
Urata, R. & D. J., Heinz, 1972. Gamma irradiation-induced mutations in sugarcane. Int. Soc. Sugarcane Technol. Proc. 14: 402–407.
Usuf, K. K. & P. M., Nair, 1974. Effect of gamma irradiation-induced mutations in sugarcane. Int. Soc. Sugarcane Technol. Proc. 16: 251–256.
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Srivastava, H.K., Tyagi, B.R. Effects of seed irradiation on yield and quality of essential oil in palmarosa (Cymbopogon martinii Stapf.). Euphytica 35, 369–380 (1986). https://doi.org/10.1007/BF00021845
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DOI: https://doi.org/10.1007/BF00021845