Summary
A reversed-phase high performance liquid chromatography (RP-HPLC) system was used to separate the storage proteins (hordeins) extracted from European barley cultivars. From a total of 38 barleys tested, 26 types of hordein patterns could be distinguished after RP-HPLC. This appears to be a marked improvement in resolution over that achieved in a similar survey of European barley cultivars using SDS polyacrylamide electrophoresis (32 hordein patterns resolved by SDS PAGE from a total of 160 spring and winter barleys tested).
Different hordein patterns were resolved by RP-HPLC within each of two groups of barley previously classified by SDS PAGE as indistinguishable within groups (three distinct patterns identified in a total of five cultivars tested from group 1A and five patterns observed among eight cultivars from group 3B). Thus RP-HPLC achieves a higher resolution than undirectional electrophoresis and promises to be a valuable aid in the identification of European barley cultivars.
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References
Bietz, J. A., 1983. Separation of cereal proteins by reversed-phase high-performance liquid chromatography. J. Chromatography 255: 219–222.
Bietz, J. A., T., Burnouff, L. A., Cobb & J. S., Wall, 1984. Wheat varietal identification and genetic analysis by reserved-phase high-performance liquid chromatography. Cereal Chemistry 61: 129–136.
Blake, T. K., S. E., Ullrich & R. A., Nilan, 1982. Mapping of the Hor-3 locus encoding D hordein in barley. Theoretical and Applied Genetics 63: 367–371.
Doll, H., 1981. Genetic possibilities for improving the nutritional quality of barley. Proceedings of the Fourth International Barley Genetics Symposium, Edinburgh. 257–263.
Doll, H. & A. H. D., Brown, 1970. Hordein variation in wild (Hordeum spontaneum) and cultivated (H. vulgare) barley. Can. J. Gen. Cytol. 21: 391–404.
Huebner, F. R. & J. A., Bietz, 1984. Separation of wheat gliadins by preparative reversed-phase high-performance liquid chromatography. Cereal Chemistry 61: 449–454.
Kruger, J. E. & B. A., Marchylo, 1985. Examination of the mobilisation of storage proteins of wheat kernels during germination by reversed phase high-performance and gel permeation chromatography. Cereal Chemistry 61: 1–5.
Marchylo, B. A. & J. E., Kruger, 1984. Identification of Canadian barley cultivars by reversed-phase high-performance liquid chromatography. Cereal Chemistry 61: 295–301.
Oliver, J. L. & J. M., Martinez-Zapiter, 1985. A genetic classification of potato cultivars based on allozyme patterns. Theoretical and Applied Genetics 69: 305–313.
Shewry, P. R., A. J., Faulks, S., Parmer & B. J., Miflin, 1980. Hordein polypeptides in relation to malting quality and to varietal identification of malted barley grain. Journal of the Institute of Brewing 86: 138–141.
Shewry, P. R., H. M., Pratt, A. J., Faulks, S., Palmer & B. J., Miflin, 1979. The storage protein (hordein) polypeptide pattern of barley (Hordeum vulgare) in relation to varietal identification and disease resistance. J. Nat. Inst. Agric. Bot. 15: 34–50.
Shewry, P. R., H. M., Pratt & B. J., Miflin, 1978. Varietal identification of single seeds of barley by analysis of the hordein polypeptides. Journal of the Food and Science of Agriculture 29: 387–396.
Smith, D. B. & P. I., Payne, 1984. A procedure for the routine determinations of electrophoretic band patterns of barley and malt endosperm proteins. Journal of the National Institute of Agricultural Botany 16: 487–493.
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Allison, M.J., Bain, H. The use of reversed-phase high performance liquid chromatography as an aid to the identification of European barley cultivars. Euphytica 35, 345–351 (1986). https://doi.org/10.1007/BF00021842
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DOI: https://doi.org/10.1007/BF00021842