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Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation

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Abstract

A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes.

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Authors and Affiliations

  1. U.A. Physiologie Intégree de l'Arbre Fruitier (INRA-Université), 4, rue Ledru, 63038, Clermont-Ferrand Cedex, France

    Jean-Philippe Galaud & Nicole Boyer

  2. Laboratoire de Biologie Cellulaire, C.N.R.S., URA 360, 24, Avenue des Landais, 63177, Aubière Cedex, France

    Jean-Jacques Lareyre

Authors
  1. Jean-Philippe Galaud
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  2. Jean-Jacques Lareyre
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  3. Nicole Boyer
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Galaud, JP., Lareyre, JJ. & Boyer, N. Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation. Plant Mol Biol 23, 839–846 (1993). https://doi.org/10.1007/BF00021538

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  • DOI: https://doi.org/10.1007/BF00021538

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