Summary
Lettuce pollen can be stored at −18°C for over 30 days without serious loss of viability. This viability was checked through in vitro germination in a liquid nutrient medium containing 40% sucrose, 100 ppm H3BO3 and 100 ppm Ca(NO3)2. 4H2O after rehydration of the pollen for 15–20 minutes in an atmosphere of 100% RH.
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References
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Eenink, A.H. Preliminary results of research on storage and in vitro germination of lettuce pollen as an aid in lettuce breeding. Euphytica 32, 521–526 (1983). https://doi.org/10.1007/BF00021464
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DOI: https://doi.org/10.1007/BF00021464