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Production of normal seeds from plants regenerated from the meristems of Arachis hypogaea and Cicer arietinum cryopreserved for 20 months

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Summary

Plants of Arachis hypogaea and Cicer arietinum regenerated from the freeze-preserved cultures of excised meristems were transferred to soil, reared to maturity, and harvested. The seeds showed normal germination, and no change in the number of chromosomes was observed. The optimal cryoability was observed in the meristems precultured for 3–7 days in 3% DMSO, followed by treatment with a combination of cryoprotectants, and thawing at 35°C. There was a maximum survival of 42% and 57% respectively in A. hypogacea and C. arietinum meristems freeze-preserved for upto 20 months. The significance of the freeze-preservation of excised meristems for the conservation of germplasm is emphasized.

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Authors and Affiliations

  1. Tissue Culture Laboratory, Punjab Agricultural University, Ludhiana, (Punjab), India

    Y. P. S. Bajaj

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  1. Y. P. S. Bajaj
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Bajaj, Y.P.S. Production of normal seeds from plants regenerated from the meristems of Arachis hypogaea and Cicer arietinum cryopreserved for 20 months. Euphytica 32, 425–430 (1983). https://doi.org/10.1007/BF00021451

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  • DOI: https://doi.org/10.1007/BF00021451

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