Abstract
In an effort to understand the mechanism of streptomycin resistance in Nicotiana plumbaginifolia, we have sequenced the chloroplast rps12 gene, a potential molecular target. We report that a streptomycin-resistant mutant isolated from protoplast cultures of N. plumbaginifolia contains an A-to-G transition at nucleotide position 149 in exon 2 of the chloroplast rps12 gene. The detected point mutation predicts a substitution of arginine for lysine in a phylogenetically conserved region.
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Hsu, CM., Yang, WP., Chen, CC. et al. A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance. Plant Mol Biol 23, 179–183 (1993). https://doi.org/10.1007/BF00021429
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DOI: https://doi.org/10.1007/BF00021429