Summary
The effect of gamma rays on parameters such as chlorophyll mutation frequency and spectrum, mutagenic effectiveness and efficiency and viable mutation frequency and spectrum were studied in M2 generation of Rhodes grass employing nine doses of gamma rays. The chlorophyll mutation frequency increased in a linear fashion at low and medium doses and was erratic at higher doses. The chlorophyll mutant spectrum ineluded albina, xantha, chlorina, viridis, tigrina, striata, albo-xantha and albo-viridis. The frequency of these mutants varied with treatments. A dose rate of 60 krad was found to be effective as well as efficient both on M1 and M2 plant basis in inducing chlorophyll mutations. The highest frequency of viable mutations was found in 50 krad treatment. Viable mutants found were: nodal variant, peduncle variant, lax ears, coxcomb ears, earhead proliferation, level of fingers, incurved fingers, gappy ears, apical sterility, partial sterility and high sterility.
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References
Bottino P. J., 1965. Radiosensitivity studies on an interspecific grass hybrid. J. Hered. 56: 225–258.
Bremer-Reinders D. E., 1962. Mutation research in canary grass. Eyphytica 11: 293–310.
Broertjes C., 1975. The development of (new) in vivo and in vitro techniques of significance for mutation breeding of vegetatively propagated crops. In. Improvement of vegetatively propagated plants through induced mutations. Proc. of Res. Coordinating Meeting. Tokai, Jpn. IAEA, Vienna, pp. 23–31.
Burton G. W., J. B. Powell & W. W. Hanna, 1974. Effect of recurrent mutagen treatments on mutation frequency and combining ability for forage yield in pearl millet (Pennisetum americanum (L) K. Schum.). Radia. Bot. 14: 323–335.
Busey P., 1980. Gamma ray dosage and mutation breeding in St. Augustinegrass. Crop Sci. 20: 181–184.
Ehrenberg L., 1955. Factors influencing radiation induced lethality, sterility and mutation in barley. Hereditas 41: 123–146.
Ehrenberg L., U. Lundquist & G. Ahnstrom, 1958. The mutagenic action of ethyline-imine in barley. Hereditas 44: 330–336.
Gowda, M. V. C., 1977. Mutagenic response of foxtail millet (Setaria italica Beauv.) to gamma rays, EMS and combined treatments, M.Sc. (Agric.) Thesis, University of Agricultural Sciences, Bangalore.
Gupta P. K. & Yashvir, 1976. Induced mutations in foxtail millet (Setaria italica Beauv.). II. Viable mutations in ear characters produced by gamma rays, EMS and DES. Theor. Appl. Genet. 48: 131–136.
Gustafsson A., 1940. The mutation system of the chlorophyll apparatus. Lunds Univ. Arskr. 36: 1–40.
Gustafsson A., 1963. Productive mutations induced in barley by ionizing radiations and chemical mutagens. Hereditas 50: 211–263.
Hutton E. M., 1961. Inter-variety variation in Rhodes grass (Chloris gayana Kunth.). J. Brit. Grassl. Soc. 16: 23–29.
Kawai T., 1969. Relative effectiveness of physical and chemical mutagens. In: Proc. Symp. on ‘Induced mutation in plant breeding’. FAO/IAEA, Washington, pp. 137–151.
Konzak C. F., T. Wagner & R. J. Foster, 1965. Efficient chemical mutagenesis. Radiat. Bot. 5: 49–70.
Krishna, G., G. Shivashankar & J. Nath, 1984. Mutagenic response of Rhodes grass (Chloris gayana Kunth.) to gamma rays. Environ. Exp. Bot. (in press).
Micke A., 1976. Bibliography. Examples of literature related to the use of induced mutations in cross-breeding. In: Induced mutations in cross-breeding. IAEA, Vienna, pp. 233–252.
Powell J. B., 1976. Induced mutations in highly heterozygous vegetatively propagated grasses. In: Induced mutations in cross-breeding. IAEA, Vienna, pp. 219–224.
Powelll J. B., G. W. Burton & J. R. Young, 1974. Mutations induced in vegetatively propagated turf bermudagrasses by gamma radiation. Crop Sci. 14: 327–330.
Powell, J. B. & J. J. Murray, 1977. The vegetative bud mutation technique for breeding improved Poa pratensis L. Proc. XIII Int. Grass. Congr., Leipzig, E. Germany.
Powell J. B. & R. W. Toler, 1980. Induced mutations in ‘Floratam’ St. Augustinegrass. Crop. Sci. 20: 644–646.
Prasad A. B., 1967. Comparison of the effect of x-rays (Soft x-rays) on the production of mutations in diploid and tetraploid species of Phalaris. Cytologia 32: 444–449.
Shivashankar G., M. Gopala Reddy, R. S. Kulkarni & R. Nazarat, 1981. Promising non-arrowing mutant of green panic. Indian J. Agri. Sci. 51: 386–388.
Sreekantaradhya R. & P. M. Madhavamenon, 1979. Induced mutagenesis in finger-millet (Eleusine coracona Gaertu.) with gamma-rays and ethyl methane sulphonate. II. chlorophyll mutation frequency and spectrum. Environ. Exp. Bot. 19: 123–126.
Sreeramulu K., 1970. Sensitivity and induction of mutations in Sorghum. Mutat. Res. 10: 197–206.
Swaminathan M. S., 1961. Effect of diplontic selection on the frequency and spectrum of mutation induced in polyploids following seed irradiation. In: Proc. Symp. on ‘Effects of ionizing radiation on seeds’. IAEA, Vienna, pp. 279–288.
Urata R. & D. J. Heinz, 1972. Gamma irradiation-induced mutations in sugarcane. Int. Soc. Sugarcane Technol. Proc. 14: 402–407.
Walker D. I. T. & N. S. Sisodia, 1969. Induction of non-flowering mutant in sugarcane (Saccharum sp.). Crop. Sci. 9: 551–552.
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Krishna, G., Shivashankar, G. & Nath, J. Mutagenic response of rhodes grass (Chloris gayana Kunth.) to gamma rays. II. Studies on second (M2) generation parameters. Euphytica 33, 517–524 (1984). https://doi.org/10.1007/BF00021152
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DOI: https://doi.org/10.1007/BF00021152