Abstract
Leptochloa fusca (L.) Kunth and Atriplex hortensis (L.) were grown on quartz sand or in liquid culture in the presence of varied concentrations of NaCl. Xylem sap was collected as (a) root pressure exudate, in L. fusca even at 100 mM NaCl, (b) by applying pressure to excised roots of L. fusca and (c) from leaves of the whole plant growing in quartz sand by pressurizing the root system. The latter procedure failed in L. fusca due to the passage of air and soil solution into the leaves. This was caused by an extensive aerenchyma in root cortex. In Atriplex hortensis remarkably high pressures were required to induce a flow of sap. The mineral cation and anion and the amino acid composition of the xylem sap obtained by the different methods was measured and is examined in view of using it for determining the flows of minerals in the whole plant and in relation to the effects of salinity. The spacious aerenchyma in roots of L. fusca has been found to persist also after prolonged exposure to dry air.
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References
Armstrong M J and Kirkby E A 1979 Estimation of potassium recirculation in tomato plants by comparison of the rates of potassium and calcium accumulation in the tops with their fluxes in the xylem stream. Plant Physiol. 63, 1143–1148.
Armstrong W 1979 Aeration in higher plants. Adv. Bot. Res. 7, 225–332.
Balling Q and Zimmermann U 1990 Comparative measurements of the xylem pressure in Nicotiana plants by means of the pressure bomb and pressure probe. Planta 182, 325–338.
Bhatti A S, Steinert S, Sarwar G, Hilpert A and Jeschke W D 1993 Ion distribution in relation to leaf age in Leptochloa fusca (L.) Kunth (Kallar grass) I. K+, Na+ Ca++ and Mg++. New Phytol. 123, 539–545.
Bowling D J F, Watson B T and Ehwald R 1985 The effect of ringing on root growth and potassium uptake by Helianthus annuus. J. Exp. Bot. 36, 290–297.
Clipson N J W and Flowers T J 1987 Salt tolerance in the halophyte Suaeda maritima (L.) Dum. The effect of the concentration of sodium in the xylem. New Phytol. 105, 359–366.
Cruz R T, Wayne R J and Drew M C 1992 Structural changes and associated reduction of hydraulic conductance in roots of Sorghum bicolor L. following exposure to water deficit. Plant Physiol. 99, 203–212.
deBoer A H, Prins H B A and Zanstra P E 1983 Bi-phasic composition of trans-root electrical potential in roots of Plantago species: involvement of spatially separated electrogenic pumps. Planta 157, 259–266.
Duarte P J P and Larsson C M 1993 Translocation of nutrients in N-limited, non-nodulated pea plants. J. Plant Physiol. 141, 182–187.
Epel B L and Bandurski R S 1990 Apoplastic domains and subdomains in the shoots of etiolated corn seedlings. Physiol. Plant. 79, 599–603.
Jeschke W D 1984 Effects of transpiration on potassium and sodium fluxes in root cells and the regulation of ion distribution between roots and shoots of barley seedlings. J. Plant Physiol. 117, 267–285.
Jeschke W D, Atkins C A and Pate J S 1985 Ion circulation via phloem and xylem between root and shoot of nodulated white lupin. J. Plant Physiol. 117, 319–330.
Jeschke W D and Pate JS 1991 Cation and chloride partitioning through xylem and phloem within the whole plant of Ricinus communis L. under conditions of salt stress. J. Exp. Bot. 42, 1105–1116.
Jeschke W D, Wolf O and Pate J S 1991 Solute exchanges from xylem to phloem in the leaf and from phloem to xylem in the root. In Recent Advances in Phloem Translocation and Assimilate Compartmentation. Eds. J LBonnemain, SDelrot, W JLucas and JDainty. pp 96–107. Ouest Editions Press Academique, Nantes.
Kutschera L and Lichtenegger E 1982 Wurzelatlas mitteleuropäischer 094 Grünlandpflanzen. I. Monocotyledoneae. G Fischer, Stuttgart, New York.
Lopushinsky W 1964 Effects of water movement on ion movement into the xylem of tomato roots. Plant Physiol. 39, 494–501.
Lynch L and Läuchli A 1984 Potassium transport in salt-stressed roots. Planta 161, 295–301.
MacRobbie E A C 1971 Phloem translocation. Facts and mechanisms. A comparative survey. Biol. Rev. 46, 429–481.
Metcalfe C R 1960 Anatomy of the Monocotyledons. I. Gramineae. Clarendon Press, Oxford.
Miller D M 1985 Studies of the root function in Zea mays, III. Xylem sap compositon at maximum root pressure provides evidence of active transport into the xylem and a measurement of the reflection coefficient of the root. Plant Physiol. 77, 162–167.
Munns R and Passioura J B 1984 Hydraulic resistance of plants. III. Effects of NaCl in barley and lupin. Aust. J. Plant Physiol. 11, 351–359.
Passioura J B 1988 Water uptake in and to roots. Annu. Rev. Plant Physiol. Mol. Biol. 39, 245–265.
Pate J S, Layzell D B and Mc Neil D L 1979 Modelling the transport and utilzation of carbon and nitrogen in a nodulated legume. Plant Physiol. 63, 730–737.
Pitman M G 1971 Uptake and transport of ions in barley seedlings. I. Estimation of chloride fluxes in cells of excised roots. Aust. J. Biol. Sci. 24, 407–421.
Pitman M G 1977 Ion transport in the xylem. Annu. Rev. Plant Physiol. 28, 71–88.
Pitman M G 1981 Ion uptake. In The Physiology and Biochemistry of Drought Resistance in Plants. Eds. L GPaleg and DAspinall. pp 71–96. Academic Press, Sydney.
Salim M and Pitman M G 1984 Pressure induced water and solute flow through plant roots. J. Exp. Bot. 35, 869–881.
Sandhu G R, Aslam Z, Salim M, Sattar A, Qureshi R H, Ahmad N and Wyn Jones R G 1981 The effect of salinity on the yield and composition of Diplachne fusca (Kallar grass). Plant Cell Environ. 4, 177–181.
Tomos A D, Leigh R A, Hinde P, Richardson P and Williams J H H 1993 Measuring water and solute relations of single cells in situ. Curr. Top. Plant Biochem. Physiol. 11, 168–171.
Wolf O and Jeschke W D 1987 Modelling of sodium and potassium flows via phloem and xylem in the shoot of salt-stressed barley. J. Plant Physiol. 128, 371–386.
Wolf O, Munns R, Tonnet M L and Jeschke W D 1991 The role of the stem in partitioning of Na and K in salt-stressed barley. J. Exp. Bot. 42, 697–704.
Yeo A R and Flowers J T 1986 Ion transport in Suaeda maritima: its relation to growth and implications for the pathway of radial transport of ions across the root. J. Exp. Bot. 37, 143–159.
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Jeschke, W.D., Klagges, S. & Bhatti, A.S. Collection and composition of xylem sap and root structure in two halophytic species. Plant Soil 172, 97–106 (1995). https://doi.org/10.1007/BF00020863
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DOI: https://doi.org/10.1007/BF00020863