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Isolation and characterization of a Tritordeum cDNA encoding S-adenosylmethionine decarboxylase that is circadian-clock-regulated

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Abstract

Sequence analysis of the two cDNA clones 47/11 and 50A which were isolated by differential screening of an explant cDNA library obtained from the monocot Tritordeum (hexaploid hybrid of diploid wild barley and tetraploid wheat lines) reveals that both clones include the same open reading frame (ORF). The sequence of this ORF shows a high degree of similarity with dicot S-adenosylmethionine decarboxylase (SAMDC) gene sequences and contains regions highly conserved in all known SAMDC sequences. It is further shown that the sequence represented by the cDNA clones 47/11 and 50A is derived from the wild barley (Hordeum chilense) genome, where it is present as a single-copy gene. Northern analyses indicate the corresponding transcript to accumulate in response to wounding and the transcript level changes with a circadian rhythm, having a peak in the middle of the light period. The periodicity continues in constant light, but is changed in constant darkness.

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Authors and Affiliations

  1. Institut für Allgemeine Botanik, AMP II, Ohnhorststrasse 18, D-22609, Hamburg, Germany

    Thomas Dresselhaus, Christine Hagel & Horst Lörz

  2. Biochemistry and Physiology Department, IARC-Rothamsted, AL5 2JQ, Harpenden, Hertfordshire, UK

    Pilar Barcelo

  3. Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, D-50931, Köln

    Klaus Humbeck

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  1. Thomas Dresselhaus
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  2. Pilar Barcelo
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  3. Christine Hagel
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  4. Horst Lörz
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  5. Klaus Humbeck
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Dresselhaus, T., Barcelo, P., Hagel, C. et al. Isolation and characterization of a Tritordeum cDNA encoding S-adenosylmethionine decarboxylase that is circadian-clock-regulated. Plant Mol Biol 30, 1021–1033 (1996). https://doi.org/10.1007/BF00020812

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  • DOI: https://doi.org/10.1007/BF00020812

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