Advertisement

Photosynthesis Research

, Volume 1, Issue 3, pp 149–162 | Cite as

Plastoquinone as a common link between photosynthesis and respiration in a blue-green alga

  • Masahiko Hirano
  • Kazuhiko Satoh
  • Sakae Katoh
Article

Abstract

The role of plastoquinone in a thermophilic blue-green alga, Shynechococcus sp., was studied by measuring reduction kinetics of cytochrome 553 which was oxidized with red flash preferentially exciting photosystem I. Sensitivity of the cytochrome reduction to DBMIB1 indicates that cytochrome 553 accepts electrons from reduced plastoquinone. Plastoquinone is in turn reduced in cells without electrons from photosystem II, since DCMU2, which inhibited methyl viologen photoreduction more strongly than DBMIB, failed to affect the cytochrome reduction. Participation of cyclic electron transport around photosystem I in cytochrome reduction in the presence of DCMU was excluded, because methyl viologen and antimycin A had no effect on the cytochrome kinetics. On the other hand, electron donation from endogenous substrates to plastoquinone was suggested from decreases in rate of the cytochrome reduction by dark starvation of cells and also from restoration of fast reduction kinetics by the addition of exogenous substrates to or by reillumination of starved cells.

KCN, which completely suppressed respiratory O2-uptake, induced a marked acceleration of the cytochrome reduction in starved cells. The poison was less or not effective in stimulating the cytochrome reduction in more extensively starved or reilluminated cells.

Results indicate that plastoquinone is functioning not only in the photosynthetic but also in the respiratory electron transport chain, thereby forming a common link between the two energy conservation systems of the blue-green alga.

Footnotes
  1. 1.

    Abbreviations: DCMU = 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DBMIB = 2,5-dib romo-3-methyl-6-isopropyl-p-benzoquinone; HOQNO = 2-n-heptyl-4-hydroxyquinoline-N-oxide

  2. 2.

    Abbreviations: DCMU = 3-(3,4-dichlorophenyl)-1,1-dimethylurea; DBMIB = 2,5-dib romo-3-methyl-6-isopropyl-p-benzoquinone; HOQNO = 2-n-heptyl-4-hydroxyquinoline-N-oxide

Key words

blue-green algae cytochrome 553 electron transport photosynthesis plastoquinone respiration 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Amesz J (1977) Plastoquinone. In Trebst A and Avron M, eds. Encyclopedia of Plant Physiology, New Series, Vol 5, pp 238–246. Berlin: Springer-Verlag.Google Scholar
  2. 2.
    Ben-Amotz A (1979) Hydrogen Metabolism. In Gibbs M and Latzko E, eds. pp 497–506. Berlin: Springer-Verlag.Google Scholar
  3. 3.
    Biggins J (1969) Respiration in blue-green algae. J Bacteriol 99: 570–575.Google Scholar
  4. 4.
    Bisalputra T, Brown DL and Weier TE (1969) Possible respiratory sites in a blue-green alga Nostoc sphaerium as demonstrated by potassium tellurite and tetranitro-blue tetrazolium reduction. J Ultrastruct Res 27: 182–197.Google Scholar
  5. 5.
    Brown AH and Webster GC (1953) The influence of light on the rate of respiration of the blue-green alga Anabaena. Am J Bot 40: 753–758.Google Scholar
  6. 6.
    Carr NG and Hallaway M (1966) Quinones of some blue-green algae. In Goodwin TW, ed. Biochemistry of chloroplasts, Vol 1, pp 159–163. New York: Academic Press.Google Scholar
  7. 7.
    Duysens LNM and Sweers HE (1963) Mechanism of the two photochemical reactions in algae as studied by means of fluorescence. In Microalgae and photosynthetic bacteria, pp 353–372. Tokyo: University of Tokyo Press.Google Scholar
  8. 8.
    Dyer DL and Gafford RD (1961) Some characteristics of a thermophilic blue-green alga. Science 134: 616–617.Google Scholar
  9. 9.
    Henninger MD, Bhagavan HN and Crane FL (1965) Comparative studies on plastoquinones. 1. Evidence for three quinones in the blue-green alga, Anacystis nidulans. Arch Biochem Biophys 110: 69–74.Google Scholar
  10. 10.
    Hoch G, Owens OH and Kok B (1963) Photosynthesis and respiration. Arch Biochem Biophys 101: 171–180.Google Scholar
  11. 11.
    Imafuku H and Katoh T (1976) Intracellular ATP level and light-induced inhibition of respiration in a blue-green alga, Anabaena variabilis. Plant Cell Physiol 17: 515–524.Google Scholar
  12. 12.
    Jones LW and Myers J (1963) A common link between photosynthesis and respiration in a blue-green alga. Nature 199: 670–672.Google Scholar
  13. 13.
    Katoh S (1959) Studies on algal cytochrome. 1. Enzymic activities pertaining to Porphyra tenera cytochrome 553 in cell-free extracts. Plant Cell Physiol 11: 29–38.Google Scholar
  14. 14.
    Katoh S (1960) Studies on algal cytochrome. II. Physico-chemical properties of crystalline Porphyra tenera cytochrome 553. Plant Cell Physiol 1: 91–98.Google Scholar
  15. 15.
    Katoh S (1961) Inhibitory effect of light on oxygen-uptake by cell-free extracts and particulate fraction of Rhodopseudomonas palustris. J Biochem 49: 126–132.Google Scholar
  16. 16.
    Koike H, Satoh K and Katoh S (1978) Effects of dibromothymoquinone and bathophenanthroline on flash-induced cytochrome f oxidation in spinach chloroplasts. Plant Cell Physiol 19: 1371–1380.Google Scholar
  17. 17.
    Koike H and Katoh S (1979) Heat-stabilities of cytochromes and ferredoxin isolated from a thermophilic blue-green alga. Plant Cell Physiol 20: 1157–1161.Google Scholar
  18. 18.
    Lightbody JJ and Korgmann DW (1966) The role of plastoquinone in the photosynthetic reactions of Anabaena variabilis. Biochim Biophys Acta 120: 57–64.Google Scholar
  19. 19.
    Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140: 315–322.Google Scholar
  20. 20.
    Margulis L (1970) Origin of eukaryotic cells. New Haven: Yale University Press.Google Scholar
  21. 21.
    Murai T and Katoh T (1975) Photosystem I-dependent oxidation of organic acids in blue-green alga, Anabaena variabilis. Plant Cell Physiol 16: 789–797.Google Scholar
  22. 22.
    Nakayama K, Yamaoka T and Katoh S (1979) Chromatographic separation of photosystem I and II from the thylakoid membrane isolated from a thermophilic blue-green alga. Plant Cell Physiol 20: 1565–1576.Google Scholar
  23. 23.
    Padan E (1979) Facultative anoxygenic photosynthetic in cyanobacteria. Ann Rev Plant Physiol 30: 27–40.Google Scholar
  24. 24.
    Peschek GA (1979) Anaerobic hydrogenase activity in Anacystis nidulans. H2-dependent photoreduction and related reactions. Biochim Biophys Acta 548: 187–202.Google Scholar
  25. 25.
    Siedow JN, Huber SC and Moreland DE (1979) Effects of dibromothymoquinone on mung bean mitochondrial electron transfer and membrane fluidity. Biochim Biophys Acta 547: 282–295.Google Scholar
  26. 26.
    Slovacek RE and Hind G (1980) Energetic factors affecting carbon dioxide fixation in isolated chloroplasts. Plant Physiol 65: 526–532.Google Scholar
  27. 27.
    Sörensen L and Halldal P (1977) Comparative analyses of action spectra of glycolate excretion (‘photorespiration’) and photosynthesis in the blue-green alga Anacystis nidulans. Photochem Photobiol 26: 511–518.Google Scholar
  28. 28.
    Trebst A and Harth E (1970) On a new inhibitor of photosynthetic electron-transport in isolated chloroplasts. Z Naturforsch 25b: 1157–1159.Google Scholar
  29. 29.
    Trebst A (1974) Energy conservation in photosynthetic electron transport of chloroplasts. Ann Rev Plant Physiol 25: 423–458.Google Scholar
  30. 30.
    Wood PM (1977) The role of c-type cytochromes in algal photosynthesis. Extraction from algae of a cytochrome similar to higher plant cytochrome f. Eur J Biochem 72: 605–612.Google Scholar
  31. 31.
    Yamaoka T, Satoh K and Katoh S (1978) Photosynthetic activites of a thermophilic blue-green alga. Plant Cell Physiol 19: 943–954.Google Scholar

Copyright information

© Martinus Nijhoff/Dr. W. Junk Publishers bv 1980

Authors and Affiliations

  • Masahiko Hirano
    • 1
  • Kazuhiko Satoh
    • 1
  • Sakae Katoh
    • 1
  1. 1.Department of Pure and Applied Sciences, College of General EducationUniversity of TokyoTokyoJapan

Personalised recommendations