Abstract
A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding β-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were detected at high frequency using in situ assays for GUS activity. Previous studies in Drosophila using analogous vectors have shown that the new patterns of transcription in many cases reflect the patterns of expression of genes adjacent to the site of vector insertion. If this is also the case in plants, the vector described here will be useful in identifying the activity of genes in different cell types and will assist in determining their function.
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References
Allen ND, Cran DG, Barton SC, Hettle S, Reik W, Surani AM: Transgenes as probes for active chromosomal domains in mouse development. Nature 333: 852–855 (1988).
André D, Colau D, Schell J, VanMontagu M, Hernalsteens J-P: Gene tagging in plants by a T-DNA insertion mutagen that generates APH(3′)II-plant gene fusions. Mol Gen Genet 204: 512–518 (1986).
Benfey PN, Ren L, Chua N-H: The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J 8: 2195–2202 (1989).
Benfey PN, Ren L, Chua N-H: Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J 9: 1677–1684 (1990).
Bellen HJ, Wilson C, Gehring WJ: Dissecting the complexity of the nervous system by enhancer detection. BioEssays 12: 199–204 (1990).
Bier E, Vaessin H, Shepherd S, Lee K, McCall K, Barbel S, Ackerman L, Carretto R, Uemura T, Grell E, Jan LY, Nung JY: Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. Genes & Development 3: 1273–1287 (1989).
Bevan M, Barnes WM, Chilton M-D: Structure and transcription of the nopaline synthase gene region of T-DNA. Nucl Acids Res 11: 369–385 (1983).
Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
Chen Z-L, Pan N-S, Beachy RN: A DNA sequence element that confers seed-specific enhancement to a consitutive promoter. EMBO J 7: 297–302 (1988).
Clarkson DT, Robards AW: The development and structure of roots. In: Torrey JG, Clarkson DT (eds) The Third Cabot Symposium, p. 241. Academic Press, London (1975).
Feldmann KA, Marks MD, Christianson ML, Quatrano RS: A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science 243: 1351–1354 (1989).
Gossler A, Joyner AL, Rossant J, Skarnes WC: Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244: 463–465 (1989).
Hayward HE: The Structure of Economic Plants. Macmillan, New York (1938).
Horsch RB, Klee HJ: Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process. Proc Natl Acad Sci USA 83: 4428–4432 (1986).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–1907 (1987).
Jefferson RA, Goldsbrough A, Bevan MW: Transcriptional regulation of a patatin-1 gene in potato. Plant Mol Biol 14: 995–1006 (1990).
Katagiri F, Lam E, Chua N-H: Two tobacco DNA binding proteins with homology to the nuclear factor CREB. Nature 340: 727–730 (1989).
Koncz C, Martini N, Mayerhofer R, Koncz-Kalman Z, Körber, Redei GP, Schell J: High-frequency T-DNA-mediated gene tagging in plants. Proc Natl Acad Sci USA 86: 8467–8471 (1989).
Kothary R, Clapoff S, Brown A, Campbell R, Peterson A, Rossant J: A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube. Nature 335: 435–437 (1988).
Kozak M: Comparison of initiation of protein synthesis in procaryotes, encaryotes and organelles. Microbial Rev 47: 1–45 (1983).
Lam E, Benfey IE, Gilmartin PN, Fang PM, Chua N-H: Site specific mutations alter in vitro factor binding and charge promoter expression pattern in transgenic plants. Proc Natl Acad Sci USA 86: 7890–7894 (1989).
Lam E, Chua NH: GT-1 binding site confers light responsive expression in transgenic tobacco. Science 248: 471–474 (1990).
vonMelchner H, Reddy S, Ruley HE: Isolation of cellular promoters by using a retrovirus promoter trap. Proc Natl Acad Sci USA 87: 3733–3737 (1990).
O'Kane CJ, Gehring WJ: Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci USA 84: 9123–9127 (1987).
Sheerman S, Bevan MW: A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors. Plant Cell Rep 7: 13–16 (1988).
Teeri TH, Herrera-Estrella L, Depicker A, vanMontagu M, Palva ET: Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene. EMBO J 5: 1755–1760 (1986).
Wilson C, Pearson RK, Bellen HJ, O'Kane CJ, Grossniklaus U, Gehring WJ: P-element-mediated enhancer detections: an efficient method for isolating and characterising developmentally regulated genes in Drosophila. Genes & Development 3: 1301–1313 (1989).
Yadav NS, Vanderleyden J, Bennett DR, Barnes WM, Chilton M-D: Short direct repeats flank the T-DNA of a nopaline Ti plasmid. Proc Natl Acad Sci USA 79: 6322–6326 (1982).
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Goldsbrough, A., Bevan, M. New patterns of gene activity in plants detected using an Agrobacterium vector. Plant Mol Biol 16, 263–269 (1991). https://doi.org/10.1007/BF00020557
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DOI: https://doi.org/10.1007/BF00020557