Skip to main content
Log in

5′-Regulatory region of Agrobacterium tumefaciens T-DNA gene 6b directs organ-specific, wound-inducible and auxin-inducible expression in transgenic tobacco

  • Short communication
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The regulatory activity of a 826 bp DNA fragment located upstream of the pTiBo542 TL-DNA gene 6b coding region was analysed in transgenic tobacco, using β-glucuronidase (gus) as a reporter gene. The region was shown to drive organ-specific, wound- and auxin-inducible expression of the reporter, the effect being dependent on the type and concentration of auxin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. An G, Costa MA, Ha S-B: Nopaline synthase promoter is wound inducible aux auxin inducible. Plant Cell 2: 225–233 (1990).

    Google Scholar 

  2. An G, Costa MA, Mitra A, Ha S-B, Marton L: Organ-specific and developmental regulation of the nopaline synthase promoter in transgenic tobacco plants. Plant Physiol 88: 547–552 (1988).

    Google Scholar 

  3. Bagyan IL, Revenkova EV, Kraev AS, Skryabin KG: Functional analysis of the 5′-flanking region of gene 6b from TL-DNA pTiBo542 in transgenic tobacco plants. Mol Biol 28: 487–492 (1994).

    Google Scholar 

  4. Bouchez D, Tokuhisa J, Llewellyn D, Dennis E, Ellis J: The ocs-element is component of the promoters of several T-DNA and plant viral genes. EMBO J 8: 4197–4204 (1989).

    Google Scholar 

  5. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).

    Google Scholar 

  6. Dymock D, Risiott R, de Pater S, Lancaster J, Tillson P, Ooms G: Regulation of Agrobacterium tumefaciens T-cyt gene expression in leaves of transgenic potato (Solanum tuberosum L. cv. Désirée) is strongly influenced by plant culture conditions. Plant Mol Biol 17: 711–725 (1991).

    Google Scholar 

  7. Fraley RT, Rogers SG, Horsch RB, Eichholtz DA, Flick JS, Fink CL, Hoffmann NL, Sanders PR: The SEV system: a new disarmed Ti plasmid vector system for plant transformation. Bio/technology 3: 629–635 (1985).

    Google Scholar 

  8. Fromm H, Katagiri F, Chua NH: An octopine synthase enhancer element directs tissue-specific expression and binds ASF-1, a factor from tobacco nuclear extracts. Plant Cell 1: 977–984 (1989).

    Google Scholar 

  9. Gelvin SB, Gordon MP, Nester EW, Aronson AI: Transcription of the Agrobacterium Ti plasmid in the bacterium and in crown gall tumors. Plasmid 6: 17–29 (1981).

    Google Scholar 

  10. Gelvin SB, Karcher SJ, Goldsbrough PB: Use of a TR-DNA promoter to express genes in plants and bacteria. Mol Gen Gene 199: 240–248 (1985).

    Google Scholar 

  11. Guevara-Garcia A, Mosqueda-Cano G, Arguello-Agtorga G, Simpson J, Herrera-Estrella L: Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements. Plant J 4: 495–505 (1993).

    Google Scholar 

  12. Hood EE, Jen G, Kayes L, Kramer J, Fraley RT, Chilton M-D: Restriction endonuclease map of pTiBo542, a potential Ti plasmid vector for genetic engineering of plants. Bio/technology 2: 702–709 (1984).

    Google Scholar 

  13. Hooykaas PJ, Schilperoort RA: Agrobacterium and plant genetic engineering. Plant Mol Biol 19: 15–38 (1992).

    Google Scholar 

  14. Inoguchi M, Kamada H, Harada H: β-Glucuronidase gene expression by the Ti-agropine synthase gene promoter is preferential to callus tissue. J Plant Physiol 136: 685–689 (1990).

    Google Scholar 

  15. Janssens A, Engler G, Zambryski P, Van Montagu M: The nopaline C58 T-DNA region is transcribed in Agrobacterium tumefaciens. Mol Gen Genet 195: 341–350 (1984).

    Google Scholar 

  16. Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).

    Google Scholar 

  17. Kim Y, Buckley K, Costa MA, An G: A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. Plant Mol Biol 24: 105–117 (1994).

    Google Scholar 

  18. Koncz C, Schell J: The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204: 383–396 (1986).

    Google Scholar 

  19. Kononowicz H, Wang YE, Habeck LL, Gelvin SB: Subdomains of the octopine synthase upstream activating element direct cell-specific expression in transgenic tobacco plants. Plant Cell 4: 17–27 (1992).

    Google Scholar 

  20. Korber H, Strizhov N, Staiger D, Feldwisch J, Olsson O, Sandberg G, Palme K, Schell J, Koncz C: T-DNA gene 5 of Agrobacterium modulates auxin response by autoregulated synthesis of a grown hormone antagonist in plants. EMBO J 10: 3983–3991 (1991).

    Google Scholar 

  21. Langridge WHR, Fitzgerald KJ, Koncz C, Schell J, Szalay AA. Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones. Proc Natl Acad Sci USA 86: 3219–3223 (1989).

    Google Scholar 

  22. Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473–497 (1962).

    Google Scholar 

  23. Neuteboom ST, Hulleman E, Schilperoort RA, Hoge JH: In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco. Plant Mol Biol 22: 923–929 (1993).

    Google Scholar 

  24. Revenkova EV, Bagyan IL, Kraev AS, Skryabin KG: Primary structure of pTiBo542 T-DNA. Mol Biol 27: 28–32 (1993).

    Google Scholar 

  25. Spanier K, Schell J, Schreier PH: A functional analysis of T-DNA gene 6b: The fine tuning of cytokinin effects on shoot development. Mol Gen Genet 219: 209–216 (1989).

    Google Scholar 

  26. Strabala TJ, Crowell DN, Amasino RM: Levels and location of expression of the Agrobacterium tumefaciens pTiA6 ipt gene promoter in transgenic tobacco. Plant Mol Biol 21: 1011–1021 (1993).

    Google Scholar 

  27. Teeri TH, Lehvaslaiho H, Franck M, Uotila J, Heino P, Palva ET, Van Montagu M, Herrera-Estrella L: Gene fusions to lacZ reveal new expression patterns of chimeric genes in transgenic plants. EMBO J. 8: 343–350 (1989).

    Google Scholar 

  28. Tinland B, Fournier P, Heckel T, Otten L: Expression of a chimaeric heat-shock-inducible Agrobacterium 6b oncogene in Nicotiana rustica. Plant Mol Biol 18: 921–930 (1992).

    Google Scholar 

  29. Tinland B, Huss B, Paulus F, Bonnard G, Otten L: Agrobacterium tumefaciens 6b genes are strain-specific and affect the activity of auxin as well as cytokinin genes. Mol Gen Genet 219: 217–224 (1989).

    Google Scholar 

  30. Tinland B, Rohfritsch O, Michler P, Otten L: Agrobacterium tumefaciens T-DNA gene 6b stimulates rol-induced root formation, permits growth at high auxin concentrations and increases root size. Mol Gen Genet 223: 1–10 (1990).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bagyan, I.L., Revenkova, E.V., Pozmogova, G.E. et al. 5′-Regulatory region of Agrobacterium tumefaciens T-DNA gene 6b directs organ-specific, wound-inducible and auxin-inducible expression in transgenic tobacco. Plant Mol Biol 29, 1299–1304 (1995). https://doi.org/10.1007/BF00020470

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00020470

Key words

Navigation