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The GATA-binding protein CGF-1 is closely related to GT-1

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Abstract

Many light-regulated genes contain a conserved GATA motif in their 5′-upstream region. We have characterized in detail the GATA-binding factor, CGF-1, which binds within a 73 bp TATA-proximal light/circadian regulatory element in the Arabidopsis cab2 promoter and to two more sites farther upstream. CGF-1 was found to be distinct from other metal-dependent GATA-binding factors, but to have the same sequence requirements for binding and similar physical and chemical properties as GT-1, a factor required for light regulation of the tobacco rbcS-3A gene. CGF-1 was found to be constitutively present in extracts and was shown to be immunologically related to GT-1. The close similarity between CGF-1 and GT-1 suggests that a GT-1-like factor is involved in the phytochrome/circadian regulation of the cab2 gene. CGF-1 and GT-1 were also found to have similar sequence specificities to another constitutively-regulated GATA factor, IBF-2b, which binds the I box region of the tomato nitrate reductase gene. Of three complexes detected using an IBF-2b-specific probe, only one was identical to CGF-1/GT-1. The other two were similar to IBF-2b, demonstrating that CGF-1/GT-1, although very similar, are actually distinct from IBF-2b. These data indicate that more than one factor can bind to the same short sequence and may indicate how constitutively present factors like GT-1 can play a role in light regulation.

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  1. Graham R. Teakle

    Present address: Centre for Plant Biochemistry and Biotechnology, University of Leeds, LS2 9JT, Leeds, UK

Authors and Affiliations

  1. NSF Center for Biological Timing, Department of Biology, University of Virginia, 22903, Charlottesville, VA, USA

    Graham R. Teakle & Steve A. Kay

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Teakle, G.R., Kay, S.A. The GATA-binding protein CGF-1 is closely related to GT-1. Plant Mol Biol 29, 1253–1266 (1995). https://doi.org/10.1007/BF00020466

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  • DOI: https://doi.org/10.1007/BF00020466

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