Photosynthesis Research

, Volume 46, Issue 1–2, pp 301–308 | Cite as

Amino acid sequences of heterotrophic and photosynthetic ferredoxins from the tomato plant (Lycopersicon esculentum Mill.)

  • Kaeko Kamide
  • Harumi Sakai
  • Koh Aoki
  • Yukika Sanada
  • Keishiro Wada
  • Laura S. Green
  • Boihon C. Yee
  • Bob B. Buchanan
Regular Paper Oxygenic Photosynthesis


Several forms (isoproteins) of ferredoxin in roots, leaves, and green and red pericarps in tomato plants (Lycopersicon esculentum Mill.) were earlier identified on the basis of N-terminal amino acid sequence and chromatographic behavior (Green et al. 1991). In the present study, a large scale preparation made possible determination of the full length amino acid sequence of the two ferredoxins from leaves. The ferredoxins characteristic of fruit and root were sequenced from the amino terminus to the 30th residue or beyond. The leaf ferredoxins were confirmed to be expressed in pericarp of both green and red fruit. The ferredoxins characteristic of fruit and root appeared to be restricted to those tissue. The results extend earlier findings in demonstrating that ferredoxin occurs in the major organs of the tomato plant where it appears to function irrespective of photosynthetic competence.

Key words

ferredoxin chloroplast chromoplast amyloplast fruit pericarp root 



Coomassie brilliant blue R-250






ferredoxin-NADP+ oxidoreductase


Fast protein liquid chromatography


High performance liquid chromatography




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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Kaeko Kamide
    • 1
  • Harumi Sakai
    • 1
  • Koh Aoki
    • 1
  • Yukika Sanada
    • 1
  • Keishiro Wada
    • 1
  • Laura S. Green
    • 2
  • Boihon C. Yee
    • 2
  • Bob B. Buchanan
    • 2
  1. 1.Department of Biology, Faculty of ScienceKanazawa UniversityKanazawaJapan
  2. 2.Department of Plant BiologyUniversity of CaliforniaBerkeleyUSA

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