Abstract
A cDNA showing high sequence similarity (>70%) to plant protein phosphatase 1 catalytic subunit variants from other species has been isolated from a cDNA library derived from mRNAs expressed in elicitor-treated suspension-cultured cells. The clone appears to be a near full-length 1431 bp with a 172 bp 5′-untranslated region and a 317 bp 3′-untranslated region. The open reading frame, determined by sequence similarity, codes for a protein with predicted M r of 35552. Alternatively an ATG situated to the 5′ end of the putative start site would increase the protein size by 6 amino acids.
The mRNA for Pvpp1 was shown to be rapidly induced by elicitor treatment of suspension-cultured cells of French bean. The cloned cDNA represents one of the few examples of a gene product that is probably involved in dephosphorylation events arising after the initial responses to biotic stress.
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Abbreviations
- PAL:
-
phenylalanine ammonia-lyase
- PP1:
-
protein phosphatase 1
- Pvpp1:
-
Phaseolus vulgaris protein phosphatase 1
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Zimmerlin, A., Jupe, S.C. & Bolwell, G.P. Molecular cloning of the cDNA encoding a stress-inducible protein phosphatase 1 (PP1) catalytic subunit from French bean (Phaseolus vulgaris L.). Plant Mol Biol 28, 363–368 (1995). https://doi.org/10.1007/BF00020386
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DOI: https://doi.org/10.1007/BF00020386