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Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus

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Abstract

Two putative protein kinase cDNA clones were isolated from Brassica napus by screening with a putative protein kinase cDNA clone of Arabidopsis thaliana. The deduced amino acid sequences show a distinct modular composition, consisting of a possible protein kinase catalytic region at the amino terminus and a highly acidic region encoded from diverged simple repeat sequences at the carboxy terminus. Comparison of the nucleotide sequences encoding this acidic region revealed a high rate of in-frame length variation, while preserving the acidic characteristics. Similar variation is also found in the non-coding regions of these clones.

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References

  1. Cretin C, Puigdomenech P: Glycine rich RNA binding proteins from Sorghum vulgare Plant Mol Biol 15: 783–785 (1990).

    Article  PubMed  Google Scholar 

  2. Hanks SK, Quinn AM, Hunter T: The protein kinase family: conserved features and deduced phylogeny of the catalytic domains Science 241: 42–52 (1988).

    PubMed  Google Scholar 

  3. Irwin SD, Keen JN, Findlay JBC, LOrd JM: The Ricinus communis 2S albumin precursor: a single preproprotein may be processed into two different heterodimeric storage proteins. Mol Gen Genet 222: 400–408 (1990).

    PubMed  Google Scholar 

  4. Konieczny A, Ausubel FM: A procedure for mapping Arabidopsis mutation using co-dominant ecotype-specific PCR-based markers. Plant J 4: 403–410 (1993).

    Article  PubMed  Google Scholar 

  5. La Spada AR, Wilson EM, Lubahn DB, Harding AE, Fischbeck KH: Androgen receptor gene mutations in X-linked spinal and bulbar muscular atrophy. Nature 352: 77–79 (1991).

    Article  PubMed  Google Scholar 

  6. Levinson G, Gutman GA: Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4: 203–21 (1987).

    PubMed  Google Scholar 

  7. Mahadevan M, Tsilfidis C, Sabourin L, Shutler G, Amemiya C, Jansen G, Neville G, Narang M, Barcelo J, O'Hoy K, Leblond S, Earle-Macdonald J, DeJong PJ, Wieringa B, Korneluk RG: Myotonic distrophy mutation: an unstable CTG repeat in the 3′ untranslated region of the gene. Science 255: 1253–1255 (1992).

    PubMed  Google Scholar 

  8. Moore GP: Slipped-mispairing and the evolution of introns. Trends Biochem Sci 8: 411–414 (1983).

    Article  Google Scholar 

  9. Morgante M, Olivieri AM: PCR-amplified microsatellites as markers in plant genetics. Plant J 3: 175–182 (1993).

    Article  PubMed  Google Scholar 

  10. Oliveira DED, Seurinck J, Inzé D, VanMontagu MV, Botterman J: Differential expression of five Arabidopsis genes encoding glycine-rich proteins Plant Cell 2: 427–436 (1990).

    Article  PubMed  Google Scholar 

  11. Pang PP, Pruitt RE, Meyerowitz EM: Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana Plant Mol Biol 11: 805–820 (1988).

    Google Scholar 

  12. Park YS, Hong SW, Oh SA, Kwak JM, Lee HH, Nam HG: Two putative protein kinase genes from Arabidopsis thaliana contain highly acidic domains. Plant Mol Biol 22: 615–624 (1993).

    PubMed  Google Scholar 

  13. Park YS, Kwak JM, Kwon OY, Kim YS, Lee DS, Cho MJ, Lee HH, Nam HG: Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing. Plant Physiol 103: 359–370 (1993).

    PubMed  Google Scholar 

  14. Tautz D: Hypervariability of sequences as a general source for polymorphic DNA markers. Nucl Acids Res 17: 6463–6471 (1989).

    PubMed  Google Scholar 

  15. Tautz D, Trick M, Dover GA: Cryptic simplicity in DNA in a major source of genetic variation. Nature 322: 652–656 (1986).

    PubMed  Google Scholar 

  16. The Huntington's disease Collaborative Research Group: A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. Cell 72: 971–983 (1993).

    Google Scholar 

  17. Treier M, Pfeifle C, Tautz D: Comparison of the gap segmentation gene hunchback between Drosophila melano-gaster and Drosophila virilis reveals novel modes of evolutionary change. EMBO J 8: 1517–1525 (1989).

    PubMed  Google Scholar 

  18. Vollbrecht E, Veit B, Sinha N, Hake S. The developmental gene Knotted-1 is a member of a maize homeobox gene family. Nature 350: 241–243 (1991).

    PubMed  Google Scholar 

  19. Zhou J, Rumeau D, Showwalter AM: Isolation and characterization of two wound-inducible tomato extensin genes. Plant Mol Biol 20: 5–17 (1992).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Life Sciences, Pohang University of Science and Technology, 790-784, Pohang, Kyungbuk, South Korea

    Yu Shin Park, Ok-kyu Song, Suk Whan Hong, June Myoung Kwak & Hong Gil Nam

  2. Plant Molecular Biology and Biotechnology Research Center, 660-701, Jinju, Kyungnam, South Korea

    Moo Je Cho & Hong Gil Nam

Authors
  1. Yu Shin Park
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  2. Ok-kyu Song
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  3. Suk Whan Hong
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  4. June Myoung Kwak
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  5. Moo Je Cho
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  6. Hong Gil Nam
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Park, Y.S., Song, Ok., Hong, S.W. et al. Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus . Plant Mol Biol 27, 829–833 (1995). https://doi.org/10.1007/BF00020237

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  • DOI: https://doi.org/10.1007/BF00020237

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