Abstract
Four new independent lines that exhibit co-suppression of an introduced cab140::tms2 gene and the native cab140 gene have been isolated in Arabidopsis thaliana. These lines are of particular interest because the homology shared between the introduced and native genes is 1.3 kb of promoter DNA that only contains 14 bp of transcribed region. Most other reported examples of co-suppression involve homologies between transcribed portions of genes. A similar line, lct, had been isolated previously from EMS-mutagenized seeds, and we concluded that this example of co-suppression was probably due to a mutation that mapped at or near the introduced cab140::tms2 gene [Brusslan JA, Karlin-Neumann GA, Huang L, Tobin EM: Plant Cell 5: 667–677 (1993)]. Our observations with these four new lines, however, suggest that an epigenetic event(s) rather than a mutation might be the cause of co-suppression in these and the lct line.
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References
Jorgensen R: Silencing of plant genes by homologous transgenes. Agbiotech News Info 4: 265N-273N (1991).
Matzke M, Matzke AJM: Genomic imprinting in plants: parental effects and trans-inactivation phenomena. Annu Rev Plant Physiol Plant Mol Biol 44: 53–76 (1993).
Napoli C, Lemieux C, Jorgensen R: Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2: 279–289 (1990).
van derKrol AR, Mur LA, Beld M, Mol JNM, Stuitje AR: Flavenoid genes in petunia: addition of a limited number of gene copies may lead to suppression of gene expression. Plant Cell 2: 291–299 (1990).
Brusslan JA, Karlin-Neumann GA, Huang L, Tobin EM: An Arabidopsis mutant with a reduced level of cab140 RNA is a result of cosuppression. Plant Cell 5: 667–677 (1993).
Karlin-Neumann GA, Brusslan JA, Tobin EM: Phytochrome control of the tms2 gene in transgenic Arabidopsis: a strategy for selecting mutants in the signal transduction pathway. Plant Cell 3: 573–582 (1991).
deCarvalho F, Gheysen G, Kushnir S, vanMontagu M, Inze D, Castresana C: Suppression of β-1,3-glucanase transgene expression in homozygous plants. EMBO J 11: 2595–2602 (1992).
Grueunbaum Y, Naveh-Many T, Cedar H, Razin A: Sequence specificity of methylation in higher plant DNA. Nature 292: 860–862 (1981).
Martinez-Zapater JM, Estelle MA, Somerville CR: A highly repeated DNA sequence in Arabidopsis thaliana. Mol Gen Genet 204: 417–423 (1986).
Simoens CR, Gielen J, vanMontagu M, Inze D: Characterization of highly repetitive sequences of Arabidopsis thaliana. Nucl Acids Res 16: 6753–6766 (1988).
Brusslan JA, Tobin EM: Light-independent developmental regulation of cab gene expression in Arabidopsis thaliana seedlings. Proc Natl Acad Sci USA 89: 7791–7795 (1992).
Inglebrecht I, Houdt HV, vanMontagu M, Depicker A: Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. Proc Natl Acad Sci USA 91: 10502–10506 (1994).
Silva AJ, Ward K, White R: Mosaic methylation in clonal tissue. Devel Biol 156: 391–398 (1993).
Feil R, Walter J, Allen ND, Reik W: Developmental control of allelic methylation in the imprinted mouse Igf2 and H19 genes. Development 120: 2933–2943 (1994).
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Brusslan, J.A., Tobin, E.M. Isolation of new promoter-mediated co-suppressed lines in Arabidopsis thaliana . Plant Mol Biol 27, 809–813 (1995). https://doi.org/10.1007/BF00020233
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DOI: https://doi.org/10.1007/BF00020233