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Promoter analysis of seed storage protein genes from Canavalia gladiata D.C.

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Abstract

A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5′-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5′-deletion mutants and examined the transient expression of β-glucuronidase reporter gene by particle bombardment and the stable expression by Agrobacterium-mediated transformation of tobacco plants. Positive regulatory elements were located in the −894/−602 and −602/−74 regions of the Con A gene, and in the −428/−376, −281/−155 and −155/−50 regions of the canavalin gene. In addition, the results suggested that the A/T-rich sequences in the 5′-upstream region of the Con A gene play a role in transcriptional activation, but that those of the canavalin gene have little effect on the gene expression. The CATGCAT/A sequence was not sufficient by itself for high levels of expression of both the Con A and canavalin genes. The canavalin polypeptide amounted to about 1% of the total extractable protein in the transgenic tobacco seeds, but the Con A polypeptide was not detected in the extractable protein.

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Authors and Affiliations

  1. Department of Biology, Tokyo Metropolitan University, Minami-ohsawa, 192-03, Hachioji, Tokyo, Japan

    Sumiko Yamamoto, Daisuke Yamauchi & Takao Minamikawa

  2. Department of Gene Sciences, Faculty of Science, Hiroshima University, 724, Higashi-Hiroshima, Japan

    Masahiro Nishihara & Hiromichi Morikawa

Authors
  1. Sumiko Yamamoto
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  2. Masahiro Nishihara
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  3. Hiromichi Morikawa
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  4. Daisuke Yamauchi
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  5. Takao Minamikawa
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Yamamoto, S., Nishihara, M., Morikawa, H. et al. Promoter analysis of seed storage protein genes from Canavalia gladiata D.C.. Plant Mol Biol 27, 729–741 (1995). https://doi.org/10.1007/BF00020226

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  • DOI: https://doi.org/10.1007/BF00020226

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