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The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues

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Abstract

Dominant mutations in the Arabidopsis ETR1 gene block the ethylene signal transduction pathway. The ETR1 gene has been cloned and sequenced. Using the ETR1 cDNA as a probe, we identified a cDNA homologue (eTAE1) from tomato. eTAE1 contains an open reading frame encoding a polypeptide of 754 amino acid residues. The nucleic acid sequence for the coding sequence in eTAE1 is 74% identical to that for ETR1, and the deduced amino acid sequence is 81% identical and 90% similar. Genomic Southern blot analysis indicates that three or more ETR1 homologues exist in tomato. RNA blots show that eTAE1 mRNA is constitutively expressed in all the tissues examined, and its accumulation in leaf abscission zones was unaffected by ethylene, silver ions (an inhibitor of ethylene action) or auxin.

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References

  1. Bleecker AB, Estelle MA, Somerville C, Kende H: Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 241: 1086–1088 (1988).

    Google Scholar 

  2. Baird LM, Reid MS, Webster BD: Anatomical and physiological effects of silver thiosulfate on ethylene-induced abscission in Coleus. J Plant Growth Red 3: 217–225 (1984).

    Google Scholar 

  3. Chang C, Meyerowitz EM: The ethylene hormone response in Arabidopsis: A eukaryotic two-component signaling system. Proc Natl Acad Sci USA 92: 4129–4133 (1995).

    PubMed  Google Scholar 

  4. Chang C, Kwok SF, Bleecker AB, Meyerowitz EM: Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators. Science 262: 539–544 (1993).

    PubMed  Google Scholar 

  5. DellaPenna D, Alexander DC, Bennett AB: Molecular cloning of tomato fruit polygalacturonase: analysis of polygalacturonase mRNA levels during ripening. Proc Natl Acad Sci USA 83: 6420–6424 (1986).

    Google Scholar 

  6. Ecker JR: The ethylene signal transduction pathway in plants. Science 268: 667–675 (1995).

    PubMed  Google Scholar 

  7. Frohman MA, Dush MK, Martin GR: Rapid production of full-length cDNAs from rare transcript: amplification using a single gene-specific oligo nucleotide primer. Proc Natl Acad Sci USA 85: 8998–9002 (1988).

    PubMed  Google Scholar 

  8. Goren R, Mattoo AK, Anderson JD: Ethylene binding during leaf development and senescence and its inhibition by silver nitrate. J Plant Physiol 117: 243–248 (1984).

    Google Scholar 

  9. Guzman P, Ecker JR: Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell 2: 513–523 (1990).

    Article  PubMed  Google Scholar 

  10. Hua J, Chang C, Sun Q, Meyerowitz EM: Ethylene insensitivity conferred by Arabidopsis ERS gene. Science 269: 1712–1714.

  11. Jackson MB, Osborne DJ: Ethylene, the natural regulator of leaf abscission. Nature 225: 1019–1022 (1970).

    Google Scholar 

  12. Kalaitzis P, Koehler SM, Tucker ML: Cloning of a tomato polygalacturonase expressed in abscission. Plant Mol Biol 28: 647–656 (1995).

    PubMed  Google Scholar 

  13. Lanahan MB, Yen HC, Giovannoni JJ, Klee H: The never ripe mutation blocks ethylene perception in tomato. Plant Cell 6: 521–530 (1994).

    Article  PubMed  Google Scholar 

  14. Maeda T, Wurgler-Murphy SM, Saito H: A two component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369: 242–245 (1994).

    Article  PubMed  Google Scholar 

  15. Mattoo AK, Suttle JC: The Plant Hormone Ethylene. CRC Press, Boca Raton, FL (1991).

    Google Scholar 

  16. Ota IM, Varshavsky A: A yeast protein similar to bacterial two-component regulators. Science 262: 566–569 (1993).

    PubMed  Google Scholar 

  17. Roman G, Lubarsky B, Kieber JJ, Rothenberg M, Ecker JR: Genetic analysis of ethylene signal transduction in Arabidopsis thaliana: five novel mutant loci integrated into a stress response pathway. Genetics 139: 1393–1409 (1995).

    PubMed  Google Scholar 

  18. Ronson CW, Nixon BT, Ausubel FM: Conserved domains in bacterial regulatory proteins that respond to environmental stimuli. Cell 49: 579–581 (1987).

    PubMed  Google Scholar 

  19. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  20. Schaller GE, Bleecker AB: Ethylene binding sites generated in yeast expressing the Arabidopsis ETR1 gene. Science 270: 1809–1811.

  21. Sexton R, Roberts JA: Cell biology of abscission. Annu Rev Plant Physiol 33: 133–162 (1982).

    Article  Google Scholar 

  22. Tucker ML, Sexton R, Del Campillo, Lewis LN: Bean abscission cellulase: characterization of a cDNA clone and regulation of gene expression by ethylene and auxin. Plant Physiol 88: 1257–1262 (1988).

    Google Scholar 

  23. Yen HC, Lee S, Tanksley SD, Lanahan MB, Klee H, Giovannoni JJ: The tomato never-ripe locus regulates ethylene-inducible gene expression and is linked to a homolog of the Arabidopsis ETR1 gene. Plant Physiol 107: 1343–1353 (1995).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Plant Molecular Biology Laboratory, USDA/ARS, Building 006, BARC-West, 20705, Beltsville, MD, USA

    Dingbo Zhou, Panagiotis Kalaitzis, Autar K. Mattoo & Mark L. Tucker

  2. Department of Horticulture, University of Maryland, 20742, College Park, MD, USA

    Dingbo Zhou

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  1. Dingbo Zhou
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  2. Panagiotis Kalaitzis
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  3. Autar K. Mattoo
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  4. Mark L. Tucker
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Zhou, D., Kalaitzis, P., Mattoo, A.K. et al. The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues. Plant Mol Biol 30, 1331–1338 (1996). https://doi.org/10.1007/BF00019564

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  • DOI: https://doi.org/10.1007/BF00019564

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