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Sequence analysis of a cDNA containing the gag and prot regions of the soybean retrovirus-like element, SIRE-1

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Abstract

SIRE-1 is a family of several hundred dispersed copies of a very large DNA element from Glycine max that has features characteristic of retroviruses and retrotransposons. A 2.4 kb SIRE-1-specific fragment was recovered from a soybean cDNA library and sequenced. The sequence contains two ORFs. Theoretical translation of ORF1 produces a gag-prot-like polyprotein containing highly conserved motifs found in retroelement nucleocapsids (CX2CX4HX4C) and aspartic proteases (LDSG). The second ORF is foreshortened. The cDNA also contains nearly 200 bp of a putative 5′ LTR just upstream of a tRNA primer-binding site.

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  1. Department of Biology, Loyola University Chicago, 6525 N. Sheridan Road, 60626, Chicago, IL, USA

    Yi-An Bi & Howard M. Laten

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  1. Yi-An Bi
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  2. Howard M. Laten
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Bi, YA., Laten, H.M. Sequence analysis of a cDNA containing the gag and prot regions of the soybean retrovirus-like element, SIRE-1 . Plant Mol Biol 30, 1315–1319 (1996). https://doi.org/10.1007/BF00019562

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  • DOI: https://doi.org/10.1007/BF00019562

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