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Cloning and characterization of a cDNA encoding a Rab1-like small GTP-binding protein from Petunia hybrida

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Abstract

The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to RB11_HUMAN and PhPCRGP3 to RAB1A_HUMAN. Using PhPCRGP3 as a probe, 8 identical clones were selected from a Petunia leaf cDNA library. They all encode the same 22.5 kDa polypeptide, PhRAB1, able to bind GTP in vitro and 72% identical to RAB1A_HUMAN. Hybridizable mRNAs encoding PhRAB1 accumulated preferentially in opened flowers.

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  1. Colette Jako

    Present address: , 7 rue de Péronne, 67 000, Strasbourg, France

Authors and Affiliations

  1. CNRS URA 573, Laboratoire de Biochimie et Physiologie Végétales, ENSAM-INRA, Place Viala, F-34060, Montpellier, France

    Colette Jako & Bernard Teyssendier de la Serve

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  1. Colette Jako
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  2. Bernard Teyssendier de la Serve
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Jako, C., de la Serve, B.T. Cloning and characterization of a cDNA encoding a Rab1-like small GTP-binding protein from Petunia hybrida . Plant Mol Biol 31, 923–926 (1996). https://doi.org/10.1007/BF00019480

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  • DOI: https://doi.org/10.1007/BF00019480

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